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The map label for this gene is sppA [H]

Identifier: 226224186

GI number: 226224186

Start: 1610317

End: 1611330

Strand: Reverse

Name: sppA [H]

Synonym: Lm4b_01596

Alternate gene names: 226224186

Gene position: 1611330-1610317 (Counterclockwise)

Preceding gene: 226224188

Following gene: 226224185

Centisome position: 55.32

GC content: 39.15

Gene sequence:

>1014_bases
ATGAACGCTAAAAGATGGATTGCACTAGGGATTGTTTTTGCACTTTTGATAGTCAGTGCGTTAGCAAAGTTTACATCAAG
TCAAATCGCTTCAAAGGAAGATAGTAGTCCGACATTTGTCGAAAGCCTATTCGCAGATACCGGCGAACTAACCGAAACAG
TAATTGAAGAAGGCGGGAATGATACAATTGCTGTATTATCTGTAGATGGCACTATTCAAGATACGGGAGATTCAGGTTCC
TTATTTAGTGAGGCTGGCTACAATCATTCTTTTTTTATGCAACAATTAGAACAAGTGAGAAATGATGACTATATCCAAGG
CGTACTGCTGTATGTTAATTCACCAGGTGGCGGTGTCATGGAATCCGCGCAAATTCGTGATAAAATTTTACAAATTCAAA
AAGAACGCAATATTCCATTCTATGTTTCCATGGGGAGTATGGCAGCATCAGGTGGATATTATATTTCCGCGCCAGCCGAC
AAAATTTTTGCAAGTAAAGAAACATTGACCGGCTCGCTTGGCGTCATTATGCAAGGGTATGACTATAGTGAACTCATGAA
AAAATTAGGTGTTTCAGATAATACCATTAAAAGTGGCGAATATAAAGACATTATGAGCGGGACAAGACCAATGACGGAAG
ACGAGAAGAAGATTATGCAGTCAATGATTGATGATTCTTATAATGAGTTCGTTAAAGTAGTTGCACAGGGCCGTGGAATG
ACTGTAGAACAAGTGCGTAAAATTGCAGATGGTCGCATATACGACGGCCGCCAAGCAAAAGAGAACGGATTGATTGATGA
ATTTGGTTATCAAGAAGATGCCCTAGAAGCTTTGAAAAAAGAACAAGGGTTAGCTGATGCGACAGTCATTCAATATGATA
CACCAGAAAACTTTAGCTCGCTATTCTCTGTAGCTGCACAAAAAATCTCTGGTCAAAATGCAGATATTTCCCAACTAATC
AAATTAACTGGCACACTTAAAGCGCCAAGAATGATGTATTTATATGGAGAATAA

Upstream 100 bases:

>100_bases
AGTAAAAAAGTGAAATGGGACACAATTTAAGAGAAAGATTGTACAAAATAAAAGAGGCTGGTATTATTACATTATCTAAT
CAGAAAAAGGGGAGAAATAA

Downstream 100 bases:

>100_bases
TGTGAGAGGAGAGGCGCACATGGCTAATGAAAATGTTACTTTTGAAAGTAAACGCCAAGTCTATCATTATGAACCACCAA
TTCAGAAAGAAGAAGCCATT

Product: protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNAKRWIALGIVFALLIVSALAKFTSSQIASKEDSSPTFVESLFADTGELTETVIEEGGNDTIAVLSVDGTIQDTGDSGS
LFSEAGYNHSFFMQQLEQVRNDDYIQGVLLYVNSPGGGVMESAQIRDKILQIQKERNIPFYVSMGSMAASGGYYISAPAD
KIFASKETLTGSLGVIMQGYDYSELMKKLGVSDNTIKSGEYKDIMSGTRPMTEDEKKIMQSMIDDSYNEFVKVVAQGRGM
TVEQVRKIADGRIYDGRQAKENGLIDEFGYQEDALEALKKEQGLADATVIQYDTPENFSSLFSVAAQKISGQNADISQLI
KLTGTLKAPRMMYLYGE

Sequences:

>Translated_337_residues
MNAKRWIALGIVFALLIVSALAKFTSSQIASKEDSSPTFVESLFADTGELTETVIEEGGNDTIAVLSVDGTIQDTGDSGS
LFSEAGYNHSFFMQQLEQVRNDDYIQGVLLYVNSPGGGVMESAQIRDKILQIQKERNIPFYVSMGSMAASGGYYISAPAD
KIFASKETLTGSLGVIMQGYDYSELMKKLGVSDNTIKSGEYKDIMSGTRPMTEDEKKIMQSMIDDSYNEFVKVVAQGRGM
TVEQVRKIADGRIYDGRQAKENGLIDEFGYQEDALEALKKEQGLADATVIQYDTPENFSSLFSVAAQKISGQNADISQLI
KLTGTLKAPRMMYLYGE
>Mature_337_residues
MNAKRWIALGIVFALLIVSALAKFTSSQIASKEDSSPTFVESLFADTGELTETVIEEGGNDTIAVLSVDGTIQDTGDSGS
LFSEAGYNHSFFMQQLEQVRNDDYIQGVLLYVNSPGGGVMESAQIRDKILQIQKERNIPFYVSMGSMAASGGYYISAPAD
KIFASKETLTGSLGVIMQGYDYSELMKKLGVSDNTIKSGEYKDIMSGTRPMTEDEKKIMQSMIDDSYNEFVKVVAQGRGM
TVEQVRKIADGRIYDGRQAKENGLIDEFGYQEDALEALKKEQGLADATVIQYDTPENFSSLFSVAAQKISGQNADISQLI
KLTGTLKAPRMMYLYGE

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=219, Percent_Identity=35.1598173515982, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787527, Length=176, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 36926; Mature: 36926

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKRWIALGIVFALLIVSALAKFTSSQIASKEDSSPTFVESLFADTGELTETVIEEGGN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCC
DTIAVLSVDGTIQDTGDSGSLFSEAGYNHSFFMQQLEQVRNDDYIQGVLLYVNSPGGGVM
CEEEEEEECCEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCH
ESAQIRDKILQIQKERNIPFYVSMGSMAASGGYYISAPADKIFASKETLTGSLGVIMQGY
HHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEEECCHHHHHHCHHHHCCCHHHEEECC
DYSELMKKLGVSDNTIKSGEYKDIMSGTRPMTEDEKKIMQSMIDDSYNEFVKVVAQGRGM
CHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCC
TVEQVRKIADGRIYDGRQAKENGLIDEFGYQEDALEALKKEQGLADATVIQYDTPENFSS
CHHHHHHHHCCCCCCCCCHHHCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHH
LFSVAAQKISGQNADISQLIKLTGTLKAPRMMYLYGE
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure
MNAKRWIALGIVFALLIVSALAKFTSSQIASKEDSSPTFVESLFADTGELTETVIEEGGN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCC
DTIAVLSVDGTIQDTGDSGSLFSEAGYNHSFFMQQLEQVRNDDYIQGVLLYVNSPGGGVM
CEEEEEEECCEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCH
ESAQIRDKILQIQKERNIPFYVSMGSMAASGGYYISAPADKIFASKETLTGSLGVIMQGY
HHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEEECCHHHHHHCHHHHCCCHHHEEECC
DYSELMKKLGVSDNTIKSGEYKDIMSGTRPMTEDEKKIMQSMIDDSYNEFVKVVAQGRGM
CHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCC
TVEQVRKIADGRIYDGRQAKENGLIDEFGYQEDALEALKKEQGLADATVIQYDTPENFSS
CHHHHHHHHCCCCCCCCCHHHCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHH
LFSVAAQKISGQNADISQLIKLTGTLKAPRMMYLYGE
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]