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The map label for this gene is argF

Identifier: 226224188

GI number: 226224188

Start: 1612344

End: 1613294

Strand: Reverse

Name: argF

Synonym: Lm4b_01598

Alternate gene names: 226224188

Gene position: 1613294-1612344 (Counterclockwise)

Preceding gene: 226224189

Following gene: 226224186

Centisome position: 55.39

GC content: 39.54

Gene sequence:

>951_bases
ATGACAATGTATGCGAAAAATAATACAGCTGGGAAAGATATGCTCAGTTTGCTTGAATGGAATAAAGAAGAATTGACAGA
TATTATTAAATTAGCTGTTGCAATGAAAACGAATCCAGCTCATTATAGTCATATTTTAAGCGGGAAAATTTTAGGAATGA
TCTTTGACAAGCCTTCTACTAGAACACGGGTTTCTTTTGAAGCCGGGATTCTGCAATTAGGTGGACAAGCGATAGTGATG
AGTTCAAAGGAATTGCAAATCGGGCGTGGTGAACCGATTAAAGATACGGCGCATGTGATGTCAGAATATATTGATGCAAT
TATGATTCGGACATTTAGTCATGAAAAAGTAGAAGAATTGGCGTATCATGCAGAAATTCCAATTATTAATGGCTTAACCG
ACCTGCATCATCCATGTCAGGCGTTAGCGGATTTAATGACTATTTATGAATGGAAAGATCAGTTGGAAGGTGTCAAACTA
GCTTATATTGGTGATGGTAACAATGTATGTCATTCATTGCTTTTAGCCGGCGCTATGGTCGGGATAGATATTTGCCTTGC
GATGCCAAAAGGGTATGAGGTCGACGAAACAATTCTTGCAAAGGCAGAAAATCTGGCGAAGCAAAGTGGTGGCAAGATTT
TCGTTACAGAAGATCCAAAACTTGCAGTGACTGATGCGGACTTTATTTATACGGATGTTTGGACAAGTATGGGGCAAGAA
GATGAAAATGCGAAACGTTTGGCGGATTTTGGCGAAAAATATCAAGTAAATGCAGAATTAGTTAGTGGTGCCAAGCCTGA
TTACCACTTCTTACATTGCTTACCAGCACATCGGGAAGAAGAAGTGACAGCTGAAATCATTGATGGCAATCACTCGGTCA
TTTATCAACAAGCGGGAAATCGGCTTCATGCACAAAAAGCATTACTAGCAGCCATCTTGGAAGCAAAATAA

Upstream 100 bases:

>100_bases
GAATCTTACCACCTTTAACCGTCAGTTATGCAGAAATCGATCAAGCCATATATTTATTGAAAAGTGTTTTGGAAAACCAA
TTGATTGGGAGTGAAGAGGG

Downstream 100 bases:

>100_bases
AAAAGTCTGGCGTAAGTGCCAGACTTTTTTATTATAAGAAATTACGTTTCACTTTATCCCAGAAAGAATTTTTAGGCAGT
TTGATAATATTGATAAAACG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTMYAKNNTAGKDMLSLLEWNKEELTDIIKLAVAMKTNPAHYSHILSGKILGMIFDKPSTRTRVSFEAGILQLGGQAIVM
SSKELQIGRGEPIKDTAHVMSEYIDAIMIRTFSHEKVEELAYHAEIPIINGLTDLHHPCQALADLMTIYEWKDQLEGVKL
AYIGDGNNVCHSLLLAGAMVGIDICLAMPKGYEVDETILAKAENLAKQSGGKIFVTEDPKLAVTDADFIYTDVWTSMGQE
DENAKRLADFGEKYQVNAELVSGAKPDYHFLHCLPAHREEEVTAEIIDGNHSVIYQQAGNRLHAQKALLAAILEAK

Sequences:

>Translated_316_residues
MTMYAKNNTAGKDMLSLLEWNKEELTDIIKLAVAMKTNPAHYSHILSGKILGMIFDKPSTRTRVSFEAGILQLGGQAIVM
SSKELQIGRGEPIKDTAHVMSEYIDAIMIRTFSHEKVEELAYHAEIPIINGLTDLHHPCQALADLMTIYEWKDQLEGVKL
AYIGDGNNVCHSLLLAGAMVGIDICLAMPKGYEVDETILAKAENLAKQSGGKIFVTEDPKLAVTDADFIYTDVWTSMGQE
DENAKRLADFGEKYQVNAELVSGAKPDYHFLHCLPAHREEEVTAEIIDGNHSVIYQQAGNRLHAQKALLAAILEAK
>Mature_315_residues
TMYAKNNTAGKDMLSLLEWNKEELTDIIKLAVAMKTNPAHYSHILSGKILGMIFDKPSTRTRVSFEAGILQLGGQAIVMS
SKELQIGRGEPIKDTAHVMSEYIDAIMIRTFSHEKVEELAYHAEIPIINGLTDLHHPCQALADLMTIYEWKDQLEGVKLA
YIGDGNNVCHSLLLAGAMVGIDICLAMPKGYEVDETILAKAENLAKQSGGKIFVTEDPKLAVTDADFIYTDVWTSMGQED
ENAKRLADFGEKYQVNAELVSGAKPDYHFLHCLPAHREEEVTAEIIDGNHSVIYQQAGNRLHAQKALLAAILEAK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=306, Percent_Identity=40.1960784313725, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=27.0096463022508, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=39.0909090909091, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1790703, Length=330, Percent_Identity=38.1818181818182, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=29.2604501607717, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994908, Length=344, Percent_Identity=27.3255813953488, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=325, Percent_Identity=27.0769230769231, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=321, Percent_Identity=34.5794392523364, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=322, Percent_Identity=27.9503105590062, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=28.4345047923323, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34939; Mature: 34808

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMYAKNNTAGKDMLSLLEWNKEELTDIIKLAVAMKTNPAHYSHILSGKILGMIFDKPST
CEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCC
RTRVSFEAGILQLGGQAIVMSSKELQIGRGEPIKDTAHVMSEYIDAIMIRTFSHEKVEEL
CEEEEHHHHHHHCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
AYHAEIPIINGLTDLHHPCQALADLMTIYEWKDQLEGVKLAYIGDGNNVCHSLLLAGAMV
HHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHH
GIDICLAMPKGYEVDETILAKAENLAKQSGGKIFVTEDPKLAVTDADFIYTDVWTSMGQE
HHHHEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHCCCC
DENAKRLADFGEKYQVNAELVSGAKPDYHFLHCLPAHREEEVTAEIIDGNHSVIYQQAGN
CHHHHHHHHCCCCEEECHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCC
RLHAQKALLAAILEAK
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMYAKNNTAGKDMLSLLEWNKEELTDIIKLAVAMKTNPAHYSHILSGKILGMIFDKPST
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCC
RTRVSFEAGILQLGGQAIVMSSKELQIGRGEPIKDTAHVMSEYIDAIMIRTFSHEKVEEL
CEEEEHHHHHHHCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
AYHAEIPIINGLTDLHHPCQALADLMTIYEWKDQLEGVKLAYIGDGNNVCHSLLLAGAMV
HHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHH
GIDICLAMPKGYEVDETILAKAENLAKQSGGKIFVTEDPKLAVTDADFIYTDVWTSMGQE
HHHHEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHCCCC
DENAKRLADFGEKYQVNAELVSGAKPDYHFLHCLPAHREEEVTAEIIDGNHSVIYQQAGN
CHHHHHHHHCCCCEEECHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCC
RLHAQKALLAAILEAK
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]