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The map label for this gene is pykA

Identifier: 226224171

GI number: 226224171

Start: 1591865

End: 1593622

Strand: Reverse

Name: pykA

Synonym: Lm4b_01581

Alternate gene names: 226224171

Gene position: 1593622-1591865 (Counterclockwise)

Preceding gene: 226224172

Following gene: 226224170

Centisome position: 54.71

GC content: 40.33

Gene sequence:

>1758_bases
ATGAAAAAAACGAAAATTGTTTGTACAATTGGTCCTGCAAGTGAGTCAGTTGACACATTAGTTCAGTTGATCGAAGCAGG
AATGAACGTTGCACGTCTTAATTTCTCTCACGGAGATTTTGAAGAGCACGGTGCGCGTATTGTAAATATCCGCGAAGCAT
CGAAAAAAACTGGTAAACAAGTGGCTATCTTACTTGATACAAAAGGTCCAGAAATCCGTACAAACGACATGGTCGGCGGA
AAATTAGAATTCCAAACAGGTGATGTTGTACGTGTTTCTATGACTCCTGTTGAAGGAACAAAAGAAAAATTCTCCGTGAC
TTACGGCGAACTTTACGATGATGTAGAAATTGGTTCTTCCATTTTACTTGATGACGGCTTAATCGGTCTTGAAGTAATCG
AAAAAGACGAAGCTAATCGCGAACTAGTAACAAAAGTACTTAACCCAGGTGTACTTAAAAATAAAAAAGGTGTTAACGTA
CCAAACGTTTCTATCAACCTACCAGGAATCACAGAAAAAGATGCTGCTGATATCCGCTTTGGTTTAGAACAAGGTATTGA
TTTTATCGCTGCATCTTTCGTACGTCGTGCTACAGATGTTCTTGAAATCACTAAAATTCTAGAAGAGCACAATGCAACTC
ACGTACAAATCATTCCTAAAATCGAAAACCAAGAAGGCGTTGATAATATCGACGAAATCTTACAAGTATCTCAAGGCCTA
ATGGTTGCTCGTGGGGACCTTGGTGTTGAAATTCCAGCTGAAGAAGTTCCGATTGTACAAAAAGAACTTATCAGAAAATG
TAATAAACTTGGTAAACCTGTTATTACTGCAACACAAATGCTTGATTCCATGCAACGTAACCCACGTCCAACTCGCGCTG
AAGCAAGTGACGTAGCGAATGCGATTTTTGATGGAACAGATGCAATTATGTTGTCCGGTGAAACAGCTGCTGGGGATTAT
CCAGTAGAAGCAGTTAAAATGATGGCGAAAATCGCTGTTCGTACAGAAGAAGTACTAGTTGCTCAAGATAAATTTGCACT
TAAACTTCACGAAAATACGGATATGACAGAAGCTATCGGTCAAGCAGTTGGCCATACTGCGAAAAACCTAAATGTACAAA
CTATCGTTGCTGCGACTCAAAGTGGTCACACAGCGCGTATGATCTCTAAATACCGTCCTAAATCTCATATCGTTGCTGTA
ACATTCAACGAGCATGTATACCGCGGTTTAGCACTTTCATGGGGTGTTTATCCTCGTCTAGCTACTCCAGTTAGCAACAC
AGATGAAATGTTCGACCTTGCAGTGAAAGAATCTCTTGCTTCTGGCGTTGCAAAACAAGGCGACTTAATTATCATCACTG
CTGGTGTTCCAGTTACTGAAAGTGGAACAACAAACGTAATGAAAATCCAATTAATTGGTGAAAAAGTTGTTAAAGGCCAA
GGTATTGGCAGCAAATCTGTTATCGGTAAAGCAATCGTTGCTAAATCCAATGCAGAAGCACTTGAAAAAGCAGAAGAAGG
CGGAATCTTAATCGTTAAAACAACGGATAAAGAAATGCTACCTGCATTCGAGAAAAGTGCAGCAGTTGTTGTGGAAGAAG
GCGGCTTGACTAGCCACGCAGCAGTTGTAGGAATCAACCTAGGCATTCCTGTCATCGTTGGCGCTAAAGATGCAACATCT
CTTGTAAAAGACGGTGAAATCATTACTGTTGATTCTCGTCAAGGTGTTGTATATAACGGCAAAACAGCAACACATTAA

Upstream 100 bases:

>100_bases
CACAAACTTACTTGTTTTACAAGGTTGTTACTGGTAAAATGTGGAAGATATGTTTATAATAAATACGAATGAATAGCTAA
GTTCTAGGAGGAGTAATAAC

Downstream 100 bases:

>100_bases
TAGTTAACTTTCTAAAAGCGTCCCGCATGCGGGACGCTTTTTTTATGCGCACGCCTGTATTGTTTTTCGATTACGCCAAA
ATAGGTTATAATAGAGAGTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MKKTKIVCTIGPASESVDTLVQLIEAGMNVARLNFSHGDFEEHGARIVNIREASKKTGKQVAILLDTKGPEIRTNDMVGG
KLEFQTGDVVRVSMTPVEGTKEKFSVTYGELYDDVEIGSSILLDDGLIGLEVIEKDEANRELVTKVLNPGVLKNKKGVNV
PNVSINLPGITEKDAADIRFGLEQGIDFIAASFVRRATDVLEITKILEEHNATHVQIIPKIENQEGVDNIDEILQVSQGL
MVARGDLGVEIPAEEVPIVQKELIRKCNKLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDY
PVEAVKMMAKIAVRTEEVLVAQDKFALKLHENTDMTEAIGQAVGHTAKNLNVQTIVAATQSGHTARMISKYRPKSHIVAV
TFNEHVYRGLALSWGVYPRLATPVSNTDEMFDLAVKESLASGVAKQGDLIIITAGVPVTESGTTNVMKIQLIGEKVVKGQ
GIGSKSVIGKAIVAKSNAEALEKAEEGGILIVKTTDKEMLPAFEKSAAVVVEEGGLTSHAAVVGINLGIPVIVGAKDATS
LVKDGEIITVDSRQGVVYNGKTATH

Sequences:

>Translated_585_residues
MKKTKIVCTIGPASESVDTLVQLIEAGMNVARLNFSHGDFEEHGARIVNIREASKKTGKQVAILLDTKGPEIRTNDMVGG
KLEFQTGDVVRVSMTPVEGTKEKFSVTYGELYDDVEIGSSILLDDGLIGLEVIEKDEANRELVTKVLNPGVLKNKKGVNV
PNVSINLPGITEKDAADIRFGLEQGIDFIAASFVRRATDVLEITKILEEHNATHVQIIPKIENQEGVDNIDEILQVSQGL
MVARGDLGVEIPAEEVPIVQKELIRKCNKLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDY
PVEAVKMMAKIAVRTEEVLVAQDKFALKLHENTDMTEAIGQAVGHTAKNLNVQTIVAATQSGHTARMISKYRPKSHIVAV
TFNEHVYRGLALSWGVYPRLATPVSNTDEMFDLAVKESLASGVAKQGDLIIITAGVPVTESGTTNVMKIQLIGEKVVKGQ
GIGSKSVIGKAIVAKSNAEALEKAEEGGILIVKTTDKEMLPAFEKSAAVVVEEGGLTSHAAVVGINLGIPVIVGAKDATS
LVKDGEIITVDSRQGVVYNGKTATH
>Mature_585_residues
MKKTKIVCTIGPASESVDTLVQLIEAGMNVARLNFSHGDFEEHGARIVNIREASKKTGKQVAILLDTKGPEIRTNDMVGG
KLEFQTGDVVRVSMTPVEGTKEKFSVTYGELYDDVEIGSSILLDDGLIGLEVIEKDEANRELVTKVLNPGVLKNKKGVNV
PNVSINLPGITEKDAADIRFGLEQGIDFIAASFVRRATDVLEITKILEEHNATHVQIIPKIENQEGVDNIDEILQVSQGL
MVARGDLGVEIPAEEVPIVQKELIRKCNKLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDY
PVEAVKMMAKIAVRTEEVLVAQDKFALKLHENTDMTEAIGQAVGHTAKNLNVQTIVAATQSGHTARMISKYRPKSHIVAV
TFNEHVYRGLALSWGVYPRLATPVSNTDEMFDLAVKESLASGVAKQGDLIIITAGVPVTESGTTNVMKIQLIGEKVVKGQ
GIGSKSVIGKAIVAKSNAEALEKAEEGGILIVKTTDKEMLPAFEKSAAVVVEEGGLTSHAAVVGINLGIPVIVGAKDATS
LVKDGEIITVDSRQGVVYNGKTATH

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=494, Percent_Identity=44.5344129554656, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI33286420, Length=494, Percent_Identity=44.5344129554656, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI33286418, Length=496, Percent_Identity=44.9596774193548, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=44.6247464503043, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=44.6247464503043, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=47.9310344827586, Blast_Score=253, Evalue=5e-67,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=47.9310344827586, Blast_Score=253, Evalue=5e-67,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=44.9579831932773, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=44.9579831932773, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=43.3179723502304, Blast_Score=162, Evalue=8e-40,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=51.4767932489451, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=38.9121338912134, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1787994, Length=132, Percent_Identity=34.8484848484849, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=45.1020408163265, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=42.798353909465, Blast_Score=350, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=42.798353909465, Blast_Score=350, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=42.5925925925926, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=42.5925925925926, Blast_Score=350, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6319279, Length=495, Percent_Identity=40.6060606060606, Blast_Score=333, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6324923, Length=499, Percent_Identity=39.4789579158317, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24648964, Length=485, Percent_Identity=43.7113402061856, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI28571814, Length=485, Percent_Identity=43.7113402061856, Blast_Score=360, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24648966, Length=447, Percent_Identity=43.6241610738255, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24581235, Length=479, Percent_Identity=36.7432150313152, Blast_Score=286, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24646914, Length=268, Percent_Identity=44.4029850746269, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62617; Mature: 62617

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPASESVDTLVQLIEAGMNVARLNFSHGDFEEHGARIVNIREASKKTGKQ
CCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHCCCEEEEEEHHHCCCCCE
VAILLDTKGPEIRTNDMVGGKLEFQTGDVVRVSMTPVEGTKEKFSVTYGELYDDVEIGSS
EEEEEECCCCCEEECCCCCCEEEEECCCEEEEEECCCCCCCHHEEEEHHHHHCHHHCCCE
ILLDDGLIGLEVIEKDEANRELVTKVLNPGVLKNKKGVNVPNVSINLPGITEKDAADIRF
EEECCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHH
GLEQGIDFIAASFVRRATDVLEITKILEEHNATHVQIIPKIENQEGVDNIDEILQVSQGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
MVARGDLGVEIPAEEVPIVQKELIRKCNKLGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGDYPVEAVKMMAKIAVRTEEVLVAQDKFALKLHENTDMTEAIG
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCHHHEEEECCCEEEEEECCCCHHHHHH
QAVGHTAKNLNVQTIVAATQSGHTARMISKYRPKSHIVAVTFNEHVYRGLALSWGVYPRL
HHHCCHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCHHHHCEEEECCCCCCC
ATPVSNTDEMFDLAVKESLASGVAKQGDLIIITAGVPVTESGTTNVMKIQLIGEKVVKGQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECHHHHCCC
GIGSKSVIGKAIVAKSNAEALEKAEEGGILIVKTTDKEMLPAFEKSAAVVVEEGGLTSHA
CCCCHHHHHHHEEECCCHHHHHHHHCCCEEEEEECCHHHCCHHCCCCEEEEECCCCCCCE
AVVGINLGIPVIVGAKDATSLVKDGEIITVDSRQGVVYNGKTATH
EEEEEECCCEEEEECCCHHHHHCCCCEEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MKKTKIVCTIGPASESVDTLVQLIEAGMNVARLNFSHGDFEEHGARIVNIREASKKTGKQ
CCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHCCCEEEEEEHHHCCCCCE
VAILLDTKGPEIRTNDMVGGKLEFQTGDVVRVSMTPVEGTKEKFSVTYGELYDDVEIGSS
EEEEEECCCCCEEECCCCCCEEEEECCCEEEEEECCCCCCCHHEEEEHHHHHCHHHCCCE
ILLDDGLIGLEVIEKDEANRELVTKVLNPGVLKNKKGVNVPNVSINLPGITEKDAADIRF
EEECCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHH
GLEQGIDFIAASFVRRATDVLEITKILEEHNATHVQIIPKIENQEGVDNIDEILQVSQGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
MVARGDLGVEIPAEEVPIVQKELIRKCNKLGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGDYPVEAVKMMAKIAVRTEEVLVAQDKFALKLHENTDMTEAIG
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCHHHEEEECCCEEEEEECCCCHHHHHH
QAVGHTAKNLNVQTIVAATQSGHTARMISKYRPKSHIVAVTFNEHVYRGLALSWGVYPRL
HHHCCHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCHHHHCEEEECCCCCCC
ATPVSNTDEMFDLAVKESLASGVAKQGDLIIITAGVPVTESGTTNVMKIQLIGEKVVKGQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECHHHHCCC
GIGSKSVIGKAIVAKSNAEALEKAEEGGILIVKTTDKEMLPAFEKSAAVVVEEGGLTSHA
CCCCHHHHHHHEEECCCHHHHHHHHCCCEEEEEECCHHHCCHHCCCCEEEEECCCCCCCE
AVVGINLGIPVIVGAKDATSLVKDGEIITVDSRQGVVYNGKTATH
EEEEEECCCEEEEECCCHHHHHCCCCEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]