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The map label for this gene is pfk

Identifier: 226224172

GI number: 226224172

Start: 1593905

End: 1594864

Strand: Reverse

Name: pfk

Synonym: Lm4b_01582

Alternate gene names: 226224172

Gene position: 1594864-1593905 (Counterclockwise)

Preceding gene: 226224173

Following gene: 226224171

Centisome position: 54.76

GC content: 39.9

Gene sequence:

>960_bases
ATGAAACGAATTGCAATTTTAACAAGTGGAGGAGACGCTCCAGGAATGAATGCAGCCACTCGTGCTGTTGTACGGAAAGC
AATCTATGAAGGACTTGAAGTTTACGGTATTAACTATGGCTTTTTAGGGCTAGTCAATGGTGATATTCGTAAACTAGAAT
TAGGTTCTGTTGGTGATTTACTTCACCGCGGAGGTACATTCCTTTATTCCGCAAGATATCCTGAATTCGCTACAGAAGAA
GGACAACTTAAAGGAATTGAACAATTGAAAAAACATCAAATCGATGGCCTTGTGGTTATTGGTGGAGATGGTTCTTATCA
CGGAGCGGAAGCACTTACAAAACGTGGATTCCCAACTATTGGTATCCCAGGAACTATTGATAATGATATTTCTGGAACTG
ATTTTACTATTGGTTTTGATACAGCGTTAAACACTGTTCTTGATGCACTTGATAAAATCCGCGATACAGCTACAAGTCAT
GAACGTACTTTCATTATTGAAGTTATGGGTCGTGACGCTGGTGATATCGCTCTTTGGTCTGGACTTGCTGGTGGTGCTGA
AGCTATCATCGTTCCAGAAGAAAGTTTTAATATGGATGATGTCGTGGATCGTTTGAACAAAGGTCGCGAACGTGGTAAAA
AACATAGTATTATCGTCGTTGCTGAGGGCGTAATGTCCGGTAATGAATTCGCTAAACAATTAGCTGAATATGGCGATTAT
CATGCACGTGTAACTGTTCTTGGACACGTTCAACGTGGTGGTAGCCCAACTGCATTTGACCGTGTACTTGCAAGCCGTCT
TGGTGCACGTTCAGTAGAATTATTGTTAGAAAACCGCGGAGGATTAGCTGTTGGAATCCGTGAAAATAGAATTGTCGAAA
ATGACATTAGTGAAATTTTGAAAGAAAAACACACACTTGATCAAAAATTATTTGATCTAGCATCTATTTTGTCGATTTAA

Upstream 100 bases:

>100_bases
TTAAAAAAGTGCTCTGTTGAAGCTTTTCATTTGACCTTTTTTCATGTAGAATAAACACATGTGATGAAAAACAATATCAT
TGTCTGAGGTGGTAAGTAAA

Downstream 100 bases:

>100_bases
AACGGCTGAAGAGCTGGCTGCCATGACAAAGCATGGTAGCCTTTCTTTGTGAAAAATTTTAGTAACGTATTAAAAATAAA
CTTTGAGGGTAAAGAAATGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIAILTSGGDAPGMNAATRAVVRKAIYEGLEVYGINYGFLGLVNGDIRKLELGSVGDLLHRGGTFLYSARYPEFATEE
GQLKGIEQLKKHQIDGLVVIGGDGSYHGAEALTKRGFPTIGIPGTIDNDISGTDFTIGFDTALNTVLDALDKIRDTATSH
ERTFIIEVMGRDAGDIALWSGLAGGAEAIIVPEESFNMDDVVDRLNKGRERGKKHSIIVVAEGVMSGNEFAKQLAEYGDY
HARVTVLGHVQRGGSPTAFDRVLASRLGARSVELLLENRGGLAVGIRENRIVENDISEILKEKHTLDQKLFDLASILSI

Sequences:

>Translated_319_residues
MKRIAILTSGGDAPGMNAATRAVVRKAIYEGLEVYGINYGFLGLVNGDIRKLELGSVGDLLHRGGTFLYSARYPEFATEE
GQLKGIEQLKKHQIDGLVVIGGDGSYHGAEALTKRGFPTIGIPGTIDNDISGTDFTIGFDTALNTVLDALDKIRDTATSH
ERTFIIEVMGRDAGDIALWSGLAGGAEAIIVPEESFNMDDVVDRLNKGRERGKKHSIIVVAEGVMSGNEFAKQLAEYGDY
HARVTVLGHVQRGGSPTAFDRVLASRLGARSVELLLENRGGLAVGIRENRIVENDISEILKEKHTLDQKLFDLASILSI
>Mature_319_residues
MKRIAILTSGGDAPGMNAATRAVVRKAIYEGLEVYGINYGFLGLVNGDIRKLELGSVGDLLHRGGTFLYSARYPEFATEE
GQLKGIEQLKKHQIDGLVVIGGDGSYHGAEALTKRGFPTIGIPGTIDNDISGTDFTIGFDTALNTVLDALDKIRDTATSH
ERTFIIEVMGRDAGDIALWSGLAGGAEAIIVPEESFNMDDVVDRLNKGRERGKKHSIIVVAEGVMSGNEFAKQLAEYGDY
HARVTVLGHVQRGGSPTAFDRVLASRLGARSVELLLENRGGLAVGIRENRIVENDISEILKEKHTLDQKLFDLASILSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=355, Percent_Identity=40.5633802816901, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=44.8717948717949, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI266453768, Length=355, Percent_Identity=40.5633802816901, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI266453748, Length=355, Percent_Identity=40.5633802816901, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI4505749, Length=355, Percent_Identity=40.5633802816901, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI48762920, Length=358, Percent_Identity=39.9441340782123, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=51.5723270440252, Blast_Score=323, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=41.1575562700965, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17558788, Length=363, Percent_Identity=31.9559228650138, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI72003601, Length=318, Percent_Identity=35.5345911949685, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=37.8299120234604, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321679, Length=357, Percent_Identity=37.2549019607843, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI28573326, Length=311, Percent_Identity=42.443729903537, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=43.4083601286174, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=43.4083601286174, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34421; Mature: 34421

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAILTSGGDAPGMNAATRAVVRKAIYEGLEVYGINYGFLGLVNGDIRKLELGSVGDL
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCEEEECCCHHHH
LHRGGTFLYSARYPEFATEEGQLKGIEQLKKHQIDGLVVIGGDGSYHGAEALTKRGFPTI
HHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCC
GIPGTIDNDISGTDFTIGFDTALNTVLDALDKIRDTATSHERTFIIEVMGRDAGDIALWS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEC
GLAGGAEAIIVPEESFNMDDVVDRLNKGRERGKKHSIIVVAEGVMSGNEFAKQLAEYGDY
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
HARVTVLGHVQRGGSPTAFDRVLASRLGARSVELLLENRGGLAVGIRENRIVENDISEIL
EEEEEEEEEECCCCCCHHHHHHHHHHHCCHHEEHHEECCCCEEEEEECCCCHHHHHHHHH
KEKHTLDQKLFDLASILSI
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIAILTSGGDAPGMNAATRAVVRKAIYEGLEVYGINYGFLGLVNGDIRKLELGSVGDL
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCEEEECCCHHHH
LHRGGTFLYSARYPEFATEEGQLKGIEQLKKHQIDGLVVIGGDGSYHGAEALTKRGFPTI
HHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCC
GIPGTIDNDISGTDFTIGFDTALNTVLDALDKIRDTATSHERTFIIEVMGRDAGDIALWS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEC
GLAGGAEAIIVPEESFNMDDVVDRLNKGRERGKKHSIIVVAEGVMSGNEFAKQLAEYGDY
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
HARVTVLGHVQRGGSPTAFDRVLASRLGARSVELLLENRGGLAVGIRENRIVENDISEIL
EEEEEEEEEECCCCCCHHHHHHHHHHHCCHHEEHHEECCCCEEEEEECCCCHHHHHHHHH
KEKHTLDQKLFDLASILSI
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]