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The map label for this gene is dnaB

Identifier: 226224162

GI number: 226224162

Start: 1582131

End: 1583507

Strand: Reverse

Name: dnaB

Synonym: Lm4b_01572

Alternate gene names: 226224162

Gene position: 1583507-1582131 (Counterclockwise)

Preceding gene: 226224163

Following gene: 226224161

Centisome position: 54.37

GC content: 39.36

Gene sequence:

>1377_bases
TTGACGGAATTTTGGATGGAGCTTCAAGCGGTAGACAGCTATCAAGTAAAAGCAAGTGGAATCTTGACCGGCGCCGACAG
AAAAATTATTACGATGTTATATCAACCGCTAATGGGGGCGGAATGTCTTGCTTTATATCAAACACTGTTCACAGAAGTCG
AAGAAAACAGACTTTGGTCAGAAGCGCATTCGCACGTCCAACTTTTAAATATGCTTGATATTAGTTTGAAAGCGTTATTC
GAGGCAAGGCTAAAATTAGAAGGCTTAGGTCTTTTAAAGAGTTATGTGAAGACCGAAAACGATCAACGCTTTTATATTTA
CGAAATCTTGCCACCGTTATCACCAGAAAAGTTTTTTTCGGATGGTCTATTAAATATTTATTTATATAGCAAAATTGGCA
ATGCGCAGTTTCAACGTTTGCGTCGTTTTTTCGCAGACGAGGCATTTGACACAGCTACATATAGCGAAATCACTCGTTCG
TTTCAAGATGTTTTCGAACCGTTTAAAGGTGGTAGTAGTGCAATTACGACTGAAACTAGTCAAAAGTTACTAGACAAAGT
GACGCCAAAAGAAATTGAATTTGATGACGCGAGTTTTGACTTTAATTTGTTTTTGAGCTTGCTTTCACCGGGAATGATTG
CTCGCGAAAAGATAACAAAAGAAGTACGCCAAACGATATTAAAACTTCACGGGATTTATCAAGTTTCTGAAAAAGATATG
CCGAGTTATTTGTATCGTGCGCTTGATGCAAATGGTGAAATCGACATCGTTTATTTGCGTAAAATCGTTCGTGAAGGCTA
CACCATCGAACACGGTGCGCCGCCAAAACTGCAAATGAAGCAGGAAACGCCTGTCGATAAAGAACCAGAAGAATCGTTAA
ATGATGAAGAAGCGCTGCAAGTATATTTAGAAAGCATCACGCCTTTTCAACTTTTGGTAGACATTTCAGACGGGGCAGTT
CCCGCTGAAACCGATTTGCGCGTTGTAGAAGAAGTCATGAACCAGCAAAATTTACCAGTTCCTGTGATGAATGTGCTGAT
TGAGTACGTGTTACTCCGGCTTGACCGGAAGCTTTCTAAAAATTACATGATGACGATCGCTGCTCACTGGAAACGAAAAA
ATGTAAAAACAGCAAAAGAAGCAATGGATCTAGCGTGGCAAGAGCACGAGAAATACAAACGACTTCAAGAAGAACCAGCC
ACACCAAAAACGAATAATTACAACCGAAATTATCAAAAAACAACGCGAAAAGAAATTTTGCCGGATTGGTTTGATAAGGA
GCAAGTTGCTCCAGCCGAAAATAAAATGACAGATAAAGAAAAACAAACACTCGAAGAACAAGTTCGTGAAATTAAAGAAC
GACTAAATAAAAGGTAG

Upstream 100 bases:

>100_bases
ACGTGCGCTTCGCTTCGGTGTATCGCCAATTTAAAGATATTAGTGTGTTTGTCGAAGAGTTAAAAGATTTAATGGAAAAA
AATAAAGATAGGTGAGTAAA

Downstream 100 bases:

>100_bases
GTGAAGAAGATGGATAACATCGAAAGAACATTAGGACAGCTTTTTGAAGGCCGCGACTTTGAAAAAGAATACCAAGGTTT
AAAACAACAAGTTCTCCATT

Product: chromosome replication initiation / membrane attachment protein DnaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTEFWMELQAVDSYQVKASGILTGADRKIITMLYQPLMGAECLALYQTLFTEVEENRLWSEAHSHVQLLNMLDISLKALF
EARLKLEGLGLLKSYVKTENDQRFYIYEILPPLSPEKFFSDGLLNIYLYSKIGNAQFQRLRRFFADEAFDTATYSEITRS
FQDVFEPFKGGSSAITTETSQKLLDKVTPKEIEFDDASFDFNLFLSLLSPGMIAREKITKEVRQTILKLHGIYQVSEKDM
PSYLYRALDANGEIDIVYLRKIVREGYTIEHGAPPKLQMKQETPVDKEPEESLNDEEALQVYLESITPFQLLVDISDGAV
PAETDLRVVEEVMNQQNLPVPVMNVLIEYVLLRLDRKLSKNYMMTIAAHWKRKNVKTAKEAMDLAWQEHEKYKRLQEEPA
TPKTNNYNRNYQKTTRKEILPDWFDKEQVAPAENKMTDKEKQTLEEQVREIKERLNKR

Sequences:

>Translated_458_residues
MTEFWMELQAVDSYQVKASGILTGADRKIITMLYQPLMGAECLALYQTLFTEVEENRLWSEAHSHVQLLNMLDISLKALF
EARLKLEGLGLLKSYVKTENDQRFYIYEILPPLSPEKFFSDGLLNIYLYSKIGNAQFQRLRRFFADEAFDTATYSEITRS
FQDVFEPFKGGSSAITTETSQKLLDKVTPKEIEFDDASFDFNLFLSLLSPGMIAREKITKEVRQTILKLHGIYQVSEKDM
PSYLYRALDANGEIDIVYLRKIVREGYTIEHGAPPKLQMKQETPVDKEPEESLNDEEALQVYLESITPFQLLVDISDGAV
PAETDLRVVEEVMNQQNLPVPVMNVLIEYVLLRLDRKLSKNYMMTIAAHWKRKNVKTAKEAMDLAWQEHEKYKRLQEEPA
TPKTNNYNRNYQKTTRKEILPDWFDKEQVAPAENKMTDKEKQTLEEQVREIKERLNKR
>Mature_457_residues
TEFWMELQAVDSYQVKASGILTGADRKIITMLYQPLMGAECLALYQTLFTEVEENRLWSEAHSHVQLLNMLDISLKALFE
ARLKLEGLGLLKSYVKTENDQRFYIYEILPPLSPEKFFSDGLLNIYLYSKIGNAQFQRLRRFFADEAFDTATYSEITRSF
QDVFEPFKGGSSAITTETSQKLLDKVTPKEIEFDDASFDFNLFLSLLSPGMIAREKITKEVRQTILKLHGIYQVSEKDMP
SYLYRALDANGEIDIVYLRKIVREGYTIEHGAPPKLQMKQETPVDKEPEESLNDEEALQVYLESITPFQLLVDISDGAVP
AETDLRVVEEVMNQQNLPVPVMNVLIEYVLLRLDRKLSKNYMMTIAAHWKRKNVKTAKEAMDLAWQEHEKYKRLQEEPAT
PKTNNYNRNYQKTTRKEILPDWFDKEQVAPAENKMTDKEKQTLEEQVREIKERLNKR

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 53322; Mature: 53190

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFWMELQAVDSYQVKASGILTGADRKIITMLYQPLMGAECLALYQTLFTEVEENRLWS
CCHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EAHSHVQLLNMLDISLKALFEARLKLEGLGLLKSYVKTENDQRFYIYEILPPLSPEKFFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
DGLLNIYLYSKIGNAQFQRLRRFFADEAFDTATYSEITRSFQDVFEPFKGGSSAITTETS
CCCEEEEEEHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHH
QKLLDKVTPKEIEFDDASFDFNLFLSLLSPGMIAREKITKEVRQTILKLHGIYQVSEKDM
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
PSYLYRALDANGEIDIVYLRKIVREGYTIEHGAPPKLQMKQETPVDKEPEESLNDEEALQ
HHHHHHHHCCCCCEEHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHCCCHHHHHH
VYLESITPFQLLVDISDGAVPAETDLRVVEEVMNQQNLPVPVMNVLIEYVLLRLDRKLSK
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
NYMMTIAAHWKRKNVKTAKEAMDLAWQEHEKYKRLQEEPATPKTNNYNRNYQKTTRKEIL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PDWFDKEQVAPAENKMTDKEKQTLEEQVREIKERLNKR
CCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEFWMELQAVDSYQVKASGILTGADRKIITMLYQPLMGAECLALYQTLFTEVEENRLWS
CHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EAHSHVQLLNMLDISLKALFEARLKLEGLGLLKSYVKTENDQRFYIYEILPPLSPEKFFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
DGLLNIYLYSKIGNAQFQRLRRFFADEAFDTATYSEITRSFQDVFEPFKGGSSAITTETS
CCCEEEEEEHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHH
QKLLDKVTPKEIEFDDASFDFNLFLSLLSPGMIAREKITKEVRQTILKLHGIYQVSEKDM
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
PSYLYRALDANGEIDIVYLRKIVREGYTIEHGAPPKLQMKQETPVDKEPEESLNDEEALQ
HHHHHHHHCCCCCEEHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHCCCHHHHHH
VYLESITPFQLLVDISDGAVPAETDLRVVEEVMNQQNLPVPVMNVLIEYVLLRLDRKLSK
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
NYMMTIAAHWKRKNVKTAKEAMDLAWQEHEKYKRLQEEPATPKTNNYNRNYQKTTRKEIL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PDWFDKEQVAPAENKMTDKEKQTLEEQVREIKERLNKR
CCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]