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The map label for this gene is folC

Identifier: 226224152

GI number: 226224152

Start: 1568548

End: 1569837

Strand: Reverse

Name: folC

Synonym: Lm4b_01562

Alternate gene names: 226224152

Gene position: 1569837-1568548 (Counterclockwise)

Preceding gene: 226224153

Following gene: 226224151

Centisome position: 53.9

GC content: 34.81

Gene sequence:

>1290_bases
ATGACATTAAATTCGTATGAAGAAGCATTAGATTGGATTCACGGAACGCTTCGTCTAGGAATAAAGCCTGGACTAGCTCG
TATGGAGTATATGATGGAAAAGCTAAATCATCCGGAAAAAGCGAATAAGTGGGTACATATTGCTGGAACAAATGGAAAAG
GCTCAACACTTACTTTTGTTAGAAATGCTCTAGAAGAAGCTGGATATAGAACGGGAACCTTCACTTCACCATACATTGAA
GTTTTTAATGAACGAATTAGTATTAATGGTGAACCGATTAGCGATGAAATGATCGTTTCATTAGCTAACCGAATTAAACC
TATTGCGGAAGAGCTAGAAAAAACTATTTATGGACCACCTTCAGAATTTGAAATTATTACAGCTATGATGTTTTTATGCT
TTGCGGAATATGTTTCCATTGATATTGGTATTATCGAAGTAGGACTAGGCGGAAGACTGGACTCTACGAATATTCTAGTG
CCACTAATTTCTGTAATTACCACGATTGGGATGGATCATATGGAATTCCTTGGGGATACAATCGAGCAAATTGCTAGTGA
AAAAGCTGGCATAATTAAGCCAGGAATACCAGTTGTTTCAGGTGCTATACAAAAAGAAGCTCAAGAAGTTATAGCTAAGA
TAGCAAAAAAGAATAAATCCAATGTTGCTCAATTAAATGAGACTTTTTTCATGCATAATGAAAATGGAAATATAACTTAT
AAAACTATATATGGAGAAGAAATAAGCAATCTTTCCATTGGGCTGCTTGGTCTTCATCAATTGAATAATGCGGCGGTAGC
TATTAAAGTATTACAGTACTTAAATACATTTTCTACCTTTGAAATTGGTTTGGCGGCAATTAAAAAAGGCTTGGAAAAAG
CATTTTGGCCAGGAAGAATGGAGAAAATCATTTCAAATCCATTTATTATGCTGGATGGAGCGCATAATCCAGAAGGTATT
AAAACATTCGCTCATTCCATCCAAAAGTATTCTGGGCCTAAGAAAATAATCGTGAGTATATTAGCTGATAAAAACTATCA
AGAAATGTTAGGAATGTTAAAGTCCATTCCCAATACAGAAATAATACTAACTACTTTTGATTATCCTAGAGCTATGATGG
CGGAAGAAGTAATACAAATAGGAAAAAATGAAGGTATTTCTTTGAACCCAGATTGGCAGCAAGTATTAGGTAATCTTTAC
GAAACGGAGACTAATACGAAAATCTTTATTACAGGATCGCTTTATTTCATTTCAGAAGTGCGGAAATATTTGTTGGCCTC
AAAACGTTAA

Upstream 100 bases:

>100_bases
TCTGTTCTTGAAAGAATTGAAACGTTAAAAGAAGTATAAAAAACGAACTGACCTTATCATGAAATCAACTGGTAGGGTCA
GTTTTAAGGTGGTTTAAATA

Downstream 100 bases:

>100_bases
TCTTTTGAATAAATCGAGTATACTGTGAGAAATAAAAAAGAACAAATAGCTAAAAAAGGATTTTCAGAAGATAAAATATA
TAAAAACACCTGTTTTCCAC

Product: Folyl-polyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTLNSYEEALDWIHGTLRLGIKPGLARMEYMMEKLNHPEKANKWVHIAGTNGKGSTLTFVRNALEEAGYRTGTFTSPYIE
VFNERISINGEPISDEMIVSLANRIKPIAEELEKTIYGPPSEFEIITAMMFLCFAEYVSIDIGIIEVGLGGRLDSTNILV
PLISVITTIGMDHMEFLGDTIEQIASEKAGIIKPGIPVVSGAIQKEAQEVIAKIAKKNKSNVAQLNETFFMHNENGNITY
KTIYGEEISNLSIGLLGLHQLNNAAVAIKVLQYLNTFSTFEIGLAAIKKGLEKAFWPGRMEKIISNPFIMLDGAHNPEGI
KTFAHSIQKYSGPKKIIVSILADKNYQEMLGMLKSIPNTEIILTTFDYPRAMMAEEVIQIGKNEGISLNPDWQQVLGNLY
ETETNTKIFITGSLYFISEVRKYLLASKR

Sequences:

>Translated_429_residues
MTLNSYEEALDWIHGTLRLGIKPGLARMEYMMEKLNHPEKANKWVHIAGTNGKGSTLTFVRNALEEAGYRTGTFTSPYIE
VFNERISINGEPISDEMIVSLANRIKPIAEELEKTIYGPPSEFEIITAMMFLCFAEYVSIDIGIIEVGLGGRLDSTNILV
PLISVITTIGMDHMEFLGDTIEQIASEKAGIIKPGIPVVSGAIQKEAQEVIAKIAKKNKSNVAQLNETFFMHNENGNITY
KTIYGEEISNLSIGLLGLHQLNNAAVAIKVLQYLNTFSTFEIGLAAIKKGLEKAFWPGRMEKIISNPFIMLDGAHNPEGI
KTFAHSIQKYSGPKKIIVSILADKNYQEMLGMLKSIPNTEIILTTFDYPRAMMAEEVIQIGKNEGISLNPDWQQVLGNLY
ETETNTKIFITGSLYFISEVRKYLLASKR
>Mature_428_residues
TLNSYEEALDWIHGTLRLGIKPGLARMEYMMEKLNHPEKANKWVHIAGTNGKGSTLTFVRNALEEAGYRTGTFTSPYIEV
FNERISINGEPISDEMIVSLANRIKPIAEELEKTIYGPPSEFEIITAMMFLCFAEYVSIDIGIIEVGLGGRLDSTNILVP
LISVITTIGMDHMEFLGDTIEQIASEKAGIIKPGIPVVSGAIQKEAQEVIAKIAKKNKSNVAQLNETFFMHNENGNITYK
TIYGEEISNLSIGLLGLHQLNNAAVAIKVLQYLNTFSTFEIGLAAIKKGLEKAFWPGRMEKIISNPFIMLDGAHNPEGIK
TFAHSIQKYSGPKKIIVSILADKNYQEMLGMLKSIPNTEIILTTFDYPRAMMAEEVIQIGKNEGISLNPDWQQVLGNLYE
TETNTKIFITGSLYFISEVRKYLLASKR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=359, Percent_Identity=28.6908077994429, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI66932990, Length=359, Percent_Identity=28.6908077994429, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI1788654, Length=410, Percent_Identity=25.3658536585366, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=343, Percent_Identity=27.6967930029155, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=343, Percent_Identity=27.6967930029155, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=343, Percent_Identity=27.6967930029155, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=433, Percent_Identity=30.4849884526559, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324815, Length=349, Percent_Identity=29.512893982808, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322718, Length=437, Percent_Identity=23.3409610983982, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24641571, Length=335, Percent_Identity=29.8507462686567, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24581568, Length=174, Percent_Identity=35.0574712643678, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47780; Mature: 47649

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNSYEEALDWIHGTLRLGIKPGLARMEYMMEKLNHPEKANKWVHIAGTNGKGSTLTFV
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHH
RNALEEAGYRTGTFTSPYIEVFNERISINGEPISDEMIVSLANRIKPIAEELEKTIYGPP
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
SEFEIITAMMFLCFAEYVSIDIGIIEVGLGGRLDSTNILVPLISVITTIGMDHMEFLGDT
CHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHH
IEQIASEKAGIIKPGIPVVSGAIQKEAQEVIAKIAKKNKSNVAQLNETFFMHNENGNITY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEEECCCCCEEE
KTIYGEEISNLSIGLLGLHQLNNAAVAIKVLQYLNTFSTFEIGLAAIKKGLEKAFWPGRM
EEEECCCCCCCEEHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EKIISNPFIMLDGAHNPEGIKTFAHSIQKYSGPKKIIVSILADKNYQEMLGMLKSIPNTE
HHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCE
IILTTFDYPRAMMAEEVIQIGKNEGISLNPDWQQVLGNLYETETNTKIFITGSLYFISEV
EEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
RKYLLASKR
HHHHHHCCC
>Mature Secondary Structure 
TLNSYEEALDWIHGTLRLGIKPGLARMEYMMEKLNHPEKANKWVHIAGTNGKGSTLTFV
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHH
RNALEEAGYRTGTFTSPYIEVFNERISINGEPISDEMIVSLANRIKPIAEELEKTIYGPP
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
SEFEIITAMMFLCFAEYVSIDIGIIEVGLGGRLDSTNILVPLISVITTIGMDHMEFLGDT
CHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHH
IEQIASEKAGIIKPGIPVVSGAIQKEAQEVIAKIAKKNKSNVAQLNETFFMHNENGNITY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEEECCCCCEEE
KTIYGEEISNLSIGLLGLHQLNNAAVAIKVLQYLNTFSTFEIGLAAIKKGLEKAFWPGRM
EEEECCCCCCCEEHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EKIISNPFIMLDGAHNPEGIKTFAHSIQKYSGPKKIIVSILADKNYQEMLGMLKSIPNTE
HHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCE
IILTTFDYPRAMMAEEVIQIGKNEGISLNPDWQQVLGNLYETETNTKIFITGSLYFISEV
EEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
RKYLLASKR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]