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The map label for this gene is nrgB [H]

Identifier: 226224118

GI number: 226224118

Start: 1533618

End: 1533983

Strand: Direct

Name: nrgB [H]

Synonym: Lm4b_01527

Alternate gene names: 226224118

Gene position: 1533618-1533983 (Clockwise)

Preceding gene: 226224117

Following gene: 226224119

Centisome position: 52.65

GC content: 40.98

Gene sequence:

>366_bases
ATGTCTGGATTAACAAAAATCGAAATCATTACTCGCCCAAACCGCTTTCACTTGTTTCAAAAAGAACTTGCGAAAATCGG
CGTAAGCGGCCTAACTGTCACAAAAGCACTCGGTACTGGGTTAGAAAAAGGATTTATCGAACTTTATCGTGGTACAAAAA
AAGAAAGCAATATTCACGAGCGAATGAAAATTGAAATCGTTGTTTCCACTGTCCCGGTTGAGGATGTACTTCGTGTCGTT
AAAGAAACGCTACGTACTGGCGAACCCGGCGACGGAAAAGTATTTATTTACTCACTTGCAGAAGTCGTTAAAATCAGCAC
TGGCGAAACCGGAATCGACGCATTACAAGATAAACCATCAAAATGA

Upstream 100 bases:

>100_bases
CTATTTTTACCAATCCGTGTAAACGAAGAACAAGAATATAAAGGACTTGATTTGACGCTGCACGGCGAAAAAGCATATCA
AGAATAAAGGAGGCAACAAT

Downstream 100 bases:

>100_bases
ATAGTTAAAATGGTGAATTAATTAAAAATGGGGCCTCTATTCTAAAAAATCAATGAGAAATGGCGTTTACCCCTTCCGCT
TCTTAGCTTTTACACTTATA

Product: nitrogen regulatory PII protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 120

Protein sequence:

>121_residues
MSGLTKIEIITRPNRFHLFQKELAKIGVSGLTVTKALGTGLEKGFIELYRGTKKESNIHERMKIEIVVSTVPVEDVLRVV
KETLRTGEPGDGKVFIYSLAEVVKISTGETGIDALQDKPSK

Sequences:

>Translated_121_residues
MSGLTKIEIITRPNRFHLFQKELAKIGVSGLTVTKALGTGLEKGFIELYRGTKKESNIHERMKIEIVVSTVPVEDVLRVV
KETLRTGEPGDGKVFIYSLAEVVKISTGETGIDALQDKPSK
>Mature_120_residues
SGLTKIEIITRPNRFHLFQKELAKIGVSGLTVTKALGTGLEKGFIELYRGTKKESNIHERMKIEIVVSTVPVEDVLRVVK
ETLRTGEPGDGKVFIYSLAEVVKISTGETGIDALQDKPSK

Specific function: Required for full induction of the nrgAB operon under conditions of ammonium limitation [H]

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=106, Percent_Identity=38.6792452830189, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786655, Length=109, Percent_Identity=35.7798165137615, Blast_Score=78, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 13326; Mature: 13195

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLTKIEIITRPNRFHLFQKELAKIGVSGLTVTKALGTGLEKGFIELYRGTKKESNIHE
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHH
RMKIEIVVSTVPVEDVLRVVKETLRTGEPGDGKVFIYSLAEVVKISTGETGIDALQDKPS
EEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEHEEHHHHHHCCCCCCCCHHHCCCCC
K
C
>Mature Secondary Structure 
SGLTKIEIITRPNRFHLFQKELAKIGVSGLTVTKALGTGLEKGFIELYRGTKKESNIHE
CCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHH
RMKIEIVVSTVPVEDVLRVVKETLRTGEPGDGKVFIYSLAEVVKISTGETGIDALQDKPS
EEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEHEEHHHHHHCCCCCCCCHHHCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]