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The map label for this gene is nrgA [H]

Identifier: 226224117

GI number: 226224117

Start: 1532399

End: 1533604

Strand: Direct

Name: nrgA [H]

Synonym: Lm4b_01526

Alternate gene names: 226224117

Gene position: 1532399-1533604 (Clockwise)

Preceding gene: 226224116

Following gene: 226224118

Centisome position: 52.61

GC content: 40.71

Gene sequence:

>1206_bases
ATGGAATCAGTATTTATGTTCTTTTGTACTTTATTGGTTTGGTTAATGACACCAGGTATTGCATTATTTTACGGTGGGAT
GGTTCGACGCAAAAATGTGTTGAGTACGGCCATGTATAGTTTTAGTTCGATGGCAATTATATCTATCCTTTGGGTGATAG
TTGGGTATTCATTAGCTTTCGCCCCTGGAAACGGCTTTATAGGAAGTTTTGACTGGACCTTCCTCCATAATGTTGGCTTC
GCAGCAAACGATACTTACTCAGATGCTATTCCACATATTCTATTCATGATGTTTCAAATGACTTTCGCAATTTTGACTGT
AGCTATCATTTCTGGGGCATTTGCAGAAAGAATGAATTTCTCCGCTTATTTGATTTTCATTATCTTATGGTCGTTACTTG
TTTATTCCCCTGTTGCTCACTGGGTTTGGGGTGACGGTGGTTGGTTACGTAATCTCGGTGCACTTGATTTCGCCGGTGGG
AATGTTGTTCATATTAGTTCAGGTGTAACAGGGTTAGTTCTTGCAATTATGATTGGACGTAGAAAAGAAGCCGATTCTGC
TTCACCTCATAATTTACCTTTAGCGCTTATTGGTGGTATTCTAGTTTGGTTTGGTTGGTATGGTTTTAATGTTGGTAGCG
CCCTTACAATTGATAATGTGGCTATGATAGCTTTTGTTAATACGAATACAGCTGCTGCTGCAGGTATTATCGGTTGGGGT
TTGGTTGAATGGTTGACGAATAAAAAGCCAACTATGCTTGGAACCATTTCCGGAGCAATTGCTGGTTTAGTATCCATTAC
CCCTGCCGCTGGATTTGTTACCGTTCCAAGTTCACTTATCATTGGTTTCCTCGGCGGAGCACTATGCTTCTGGGCTGTGT
TCTGGTTGAAAGGAAAAGTAAAATATGATGATGCACTTGATGCATTCGGACTTCATGGAATCGGAGGTATTTGGGGTGGT
ATCGCAACCGGCCTTTTCGCCACAACAAAAGTAAATGAAGCTGGAGCAGACGGATTATTCTACGGCAATGCTTCTCTCGT
AGTAAAACAATTAATCGCCATTGGTTCAACGGTAGCATATGTGGCTATAGCGACAGCAATAATCGTCGTAATTATCAAAC
TATTTTTACCAATCCGTGTAAACGAAGAACAAGAATATAAAGGACTTGATTTGACGCTGCACGGCGAAAAAGCATATCAA
GAATAA

Upstream 100 bases:

>100_bases
AATTGATAAAATATTCTTGCCTTTATTAAAATATAGTGTATTATAAACGCATAAATAATATATTACATGTTAGTTTATCT
AACAAAAAGGAGAGTTGAGT

Downstream 100 bases:

>100_bases
AGGAGGCAACAATATGTCTGGATTAACAAAAATCGAAATCATTACTCGCCCAAACCGCTTTCACTTGTTTCAAAAAGAAC
TTGCGAAAATCGGCGTAAGC

Product: ammonium transporter NrgA

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MESVFMFFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAFAPGNGFIGSFDWTFLHNVGF
AANDTYSDAIPHILFMMFQMTFAILTVAIISGAFAERMNFSAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGG
NVVHISSGVTGLVLAIMIGRRKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMIAFVNTNTAAAAGIIGWG
LVEWLTNKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKVKYDDALDAFGLHGIGGIWGG
IATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAYVAIATAIIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQ
E

Sequences:

>Translated_401_residues
MESVFMFFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAFAPGNGFIGSFDWTFLHNVGF
AANDTYSDAIPHILFMMFQMTFAILTVAIISGAFAERMNFSAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGG
NVVHISSGVTGLVLAIMIGRRKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMIAFVNTNTAAAAGIIGWG
LVEWLTNKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKVKYDDALDAFGLHGIGGIWGG
IATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAYVAIATAIIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQ
E
>Mature_401_residues
MESVFMFFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAFAPGNGFIGSFDWTFLHNVGF
AANDTYSDAIPHILFMMFQMTFAILTVAIISGAFAERMNFSAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGG
NVVHISSGVTGLVLAIMIGRRKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMIAFVNTNTAAAAGIIGWG
LVEWLTNKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKVKYDDALDAFGLHGIGGIWGG
IATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAYVAIATAIIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQ
E

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=47.1604938271605, Blast_Score=330, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=29.6551724137931, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=420, Percent_Identity=27.8571428571429, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=436, Percent_Identity=28.2110091743119, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=317, Percent_Identity=30.9148264984227, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=408, Percent_Identity=37.9901960784314, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6325396, Length=409, Percent_Identity=35.9413202933985, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321559, Length=411, Percent_Identity=35.7664233576642, Blast_Score=229, Evalue=6e-61,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=30.2439024390244, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43005; Mature: 43005

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVFMFFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAF
CCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
APGNGFIGSFDWTFLHNVGFAANDTYSDAIPHILFMMFQMTFAILTVAIISGAFAERMNF
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGGNVVHISSGVTGLVLAIMIGR
HHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCCEEECCCCEEEECCCHHHHHHHHHHCC
RKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMIAFVNTNTAAAAGIIGWG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCCHHHHHHHHHH
LVEWLTNKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKV
HHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
KYDDALDAFGLHGIGGIWGGIATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAY
CCCHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEECCHHHHHHHHHHHCCHHHH
VAIATAIIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHCCC
>Mature Secondary Structure
MESVFMFFCTLLVWLMTPGIALFYGGMVRRKNVLSTAMYSFSSMAIISILWVIVGYSLAF
CCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
APGNGFIGSFDWTFLHNVGFAANDTYSDAIPHILFMMFQMTFAILTVAIISGAFAERMNF
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAYLIFIILWSLLVYSPVAHWVWGDGGWLRNLGALDFAGGNVVHISSGVTGLVLAIMIGR
HHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCCEEECCCCEEEECCCHHHHHHHHHHCC
RKEADSASPHNLPLALIGGILVWFGWYGFNVGSALTIDNVAMIAFVNTNTAAAAGIIGWG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCCHHHHHHHHHH
LVEWLTNKKPTMLGTISGAIAGLVSITPAAGFVTVPSSLIIGFLGGALCFWAVFWLKGKV
HHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
KYDDALDAFGLHGIGGIWGGIATGLFATTKVNEAGADGLFYGNASLVVKQLIAIGSTVAY
CCCHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEECCHHHHHHHHHHHCCHHHH
VAIATAIIVVIIKLFLPIRVNEEQEYKGLDLTLHGEKAYQE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]