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The map label for this gene is dgkA [H]

Identifier: 226224065

GI number: 226224065

Start: 1480913

End: 1481308

Strand: Reverse

Name: dgkA [H]

Synonym: Lm4b_01474

Alternate gene names: 226224065

Gene position: 1481308-1480913 (Counterclockwise)

Preceding gene: 226224066

Following gene: 226224064

Centisome position: 50.86

GC content: 36.36

Gene sequence:

>396_bases
ATGCTTATGGACTTGAAAGATAGAAAATACAAACGTAGTAAAAATTATGCAGAATCTTTTCATCATGCTTTTACTGGACT
AAAAACTGCTTTTTTAGAAGAACGTAATATGAGATTTCATACTTTCGCAGCGCTTGCTGTTGTCATCTGTGGCTTTTTCT
TTCATGTAACTAAATCTGAGTGGATTTTATTAATCCTTTCTATTTTTGGTGTGTTAACGCTAGAGATGGTGAACACAGCG
ATTGAACGAGCGGTAGATGTGGCGACAGAACAATACATTGATGAGGCGAAAAAAGCGAAGGATGTAGCTGCCGGAGCGGT
ACTGTTAGCAGCAATTGTAGCAAGTTTTATTGGACTGATTATATTCATACCATACTTTTGCCAGATGTTTTTATAA

Upstream 100 bases:

>100_bases
GGTTTCTTAGCAGTTCATGGATTGCTGCACTTGCTTGGGTATGATCATATGGAGCCTGACGAAGAGAAAATCATGTTTGG
CTTACAAAAAGAAGTGTTGG

Downstream 100 bases:

>100_bases
AAAGAAGGGATTGTGACTTGGTAAATGAAAGAAAATAATTTTATCTCACTCGCTAAACAAGCAAGAGAATTTGCGTATGT
TCCTTACTCGAAATTTCCAG

Product: diacylglycerol kinase

Products: NA

Alternate protein names: UdpK [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MLMDLKDRKYKRSKNYAESFHHAFTGLKTAFLEERNMRFHTFAALAVVICGFFFHVTKSEWILLILSIFGVLTLEMVNTA
IERAVDVATEQYIDEAKKAKDVAAGAVLLAAIVASFIGLIIFIPYFCQMFL

Sequences:

>Translated_131_residues
MLMDLKDRKYKRSKNYAESFHHAFTGLKTAFLEERNMRFHTFAALAVVICGFFFHVTKSEWILLILSIFGVLTLEMVNTA
IERAVDVATEQYIDEAKKAKDVAAGAVLLAAIVASFIGLIIFIPYFCQMFL
>Mature_131_residues
MLMDLKDRKYKRSKNYAESFHHAFTGLKTAFLEERNMRFHTFAALAVVICGFFFHVTKSEWILLILSIFGVLTLEMVNTA
IERAVDVATEQYIDEAKKAKDVAAGAVLLAAIVASFIGLIIFIPYFCQMFL

Specific function: Catalyzes the phosphorylation of undecaprenol, which is probably the primary physiological substrate. Is also able to phosphorylate diacylglycerol, albeit with very low efficiency. May play a role in adaptability to environmental stress conditions such as

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.66 [H]

Molecular weight: Translated: 14877; Mature: 14877

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01069 DAGK_PROKAR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMDLKDRKYKRSKNYAESFHHAFTGLKTAFLEERNMRFHTFAALAVVICGFFFHVTKSE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
WILLILSIFGVLTLEMVNTAIERAVDVATEQYIDEAKKAKDVAAGAVLLAAIVASFIGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFIPYFCQMFL
HHHHHHHHHHC
>Mature Secondary Structure
MLMDLKDRKYKRSKNYAESFHHAFTGLKTAFLEERNMRFHTFAALAVVICGFFFHVTKSE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
WILLILSIFGVLTLEMVNTAIERAVDVATEQYIDEAKKAKDVAAGAVLLAAIVASFIGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFIPYFCQMFL
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8407794; 9422608; 12397186 [H]