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The map label for this gene is nfo [H]

Identifier: 226224050

GI number: 226224050

Start: 1464881

End: 1465774

Strand: Reverse

Name: nfo [H]

Synonym: Lm4b_01459

Alternate gene names: 226224050

Gene position: 1465774-1464881 (Counterclockwise)

Preceding gene: 226224051

Following gene: 226224049

Centisome position: 50.32

GC content: 39.04

Gene sequence:

>894_bases
ATGCTAAGATTAGGTTCTCATGTATCAATGAGTGGTAAGAAAATGCTTCTTGGCGCAAGTGAAGAGGCAGCTTCTTATGG
CTCAAATACGTTTATGATTTATACTGGCGCTCCGCAAAACACGCGTAGAAAGCCAATTGAAGAATTAAATATTGAAGCCG
GCTTAGAACACATGAAAGCACATGATATGGCTGACATCGTCGTTCACGCGCCGTATATCATCAATATTGGCAATTCAGTG
AAGCCAGAAACCTTTGAACTAGGTGTTAATTTCTTGCAATCTGAAATTGAACGTACGCGTGCGCTTGGTGCCAAGCAAAT
CGTACTTCATCCTGGCGCTCATGTTGGCGAAGGGGCAGACAAAGGGATTAAACAAATTATCCAAGGTCTTAACGAAGCAT
TAATACATGATCAAGATGTACAAATTGCTTTAGAAACAATGGCTGGAAAAGGTTCTGAATGCGGACGTACTTTTGAAGAA
ATTGCTCAAATTATTGATGGCGTAACGCATAATGAATTATTATCCGTTACTTTCGATACTTGTCACACACATGATGCTGG
TTACGATATTGTAAATGATTTTGACGGGGTGTTAAATGAATTCGATAAAATTATCGGTGTTGATCGCTTAAAAGTGTTAC
ATATCAATGATAGCAAGAACGAATGTGGTGCACATAAAGACCGACACGCTAACATTGGTTTCGGTCACATTGGCTTTGAC
GCACTTCATTATATTGTGCATCATCCGCAATTAGCGGACGTCCCGAAAATCCTTGAAACACCTTATGTTGGTGAAGATAA
GGCATCGAAAAAAGCACCATATAAATGGGAAATCGCGATGTTAAGAAATGGCGAATTTGACCCAGATTTATTAAATAAAA
TCCAAAATAGTTAA

Upstream 100 bases:

>100_bases
ACAAAAAGGTAAACCAAACTATAAAAAGAAAATTAACTACAAAATGAACGAAATCAAACGTCGTGAAAGACGGAAAAAAC
GATAAGTGAGGAATGAATTT

Downstream 100 bases:

>100_bases
AAAGGAGACGAAAATAATGACAAAAACACTTGTATTCGGTCATAAAAATCCAGATACAGATACGATTTGTTCTGCCATTA
GTTACGCAGAATTAAAAAAA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLRLGSHVSMSGKKMLLGASEEAASYGSNTFMIYTGAPQNTRRKPIEELNIEAGLEHMKAHDMADIVVHAPYIINIGNSV
KPETFELGVNFLQSEIERTRALGAKQIVLHPGAHVGEGADKGIKQIIQGLNEALIHDQDVQIALETMAGKGSECGRTFEE
IAQIIDGVTHNELLSVTFDTCHTHDAGYDIVNDFDGVLNEFDKIIGVDRLKVLHINDSKNECGAHKDRHANIGFGHIGFD
ALHYIVHHPQLADVPKILETPYVGEDKASKKAPYKWEIAMLRNGEFDPDLLNKIQNS

Sequences:

>Translated_297_residues
MLRLGSHVSMSGKKMLLGASEEAASYGSNTFMIYTGAPQNTRRKPIEELNIEAGLEHMKAHDMADIVVHAPYIINIGNSV
KPETFELGVNFLQSEIERTRALGAKQIVLHPGAHVGEGADKGIKQIIQGLNEALIHDQDVQIALETMAGKGSECGRTFEE
IAQIIDGVTHNELLSVTFDTCHTHDAGYDIVNDFDGVLNEFDKIIGVDRLKVLHINDSKNECGAHKDRHANIGFGHIGFD
ALHYIVHHPQLADVPKILETPYVGEDKASKKAPYKWEIAMLRNGEFDPDLLNKIQNS
>Mature_297_residues
MLRLGSHVSMSGKKMLLGASEEAASYGSNTFMIYTGAPQNTRRKPIEELNIEAGLEHMKAHDMADIVVHAPYIINIGNSV
KPETFELGVNFLQSEIERTRALGAKQIVLHPGAHVGEGADKGIKQIIQGLNEALIHDQDVQIALETMAGKGSECGRTFEE
IAQIIDGVTHNELLSVTFDTCHTHDAGYDIVNDFDGVLNEFDKIIGVDRLKVLHINDSKNECGAHKDRHANIGFGHIGFD
ALHYIVHHPQLADVPKILETPYVGEDKASKKAPYKWEIAMLRNGEFDPDLLNKIQNS

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=266, Percent_Identity=32.7067669172932, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=33.0798479087452, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322735, Length=265, Percent_Identity=30.188679245283, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 32748; Mature: 32748

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLGSHVSMSGKKMLLGASEEAASYGSNTFMIYTGAPQNTRRKPIEELNIEAGLEHMKA
CCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCHHHHCHHHHHHHHHH
HDMADIVVHAPYIINIGNSVKPETFELGVNFLQSEIERTRALGAKQIVLHPGAHVGEGAD
HHHHHHEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
KGIKQIIQGLNEALIHDQDVQIALETMAGKGSECGRTFEEIAQIIDGVTHNELLSVTFDT
HHHHHHHHHHHHHHCCCCCHHHHEEHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHH
CHTHDAGYDIVNDFDGVLNEFDKIIGVDRLKVLHINDSKNECGAHKDRHANIGFGHIGFD
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
ALHYIVHHPQLADVPKILETPYVGEDKASKKAPYKWEIAMLRNGEFDPDLLNKIQNS
HHHHHHCCCCHHCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MLRLGSHVSMSGKKMLLGASEEAASYGSNTFMIYTGAPQNTRRKPIEELNIEAGLEHMKA
CCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCHHHHCHHHHHHHHHH
HDMADIVVHAPYIINIGNSVKPETFELGVNFLQSEIERTRALGAKQIVLHPGAHVGEGAD
HHHHHHEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
KGIKQIIQGLNEALIHDQDVQIALETMAGKGSECGRTFEEIAQIIDGVTHNELLSVTFDT
HHHHHHHHHHHHHHCCCCCHHHHEEHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHH
CHTHDAGYDIVNDFDGVLNEFDKIIGVDRLKVLHINDSKNECGAHKDRHANIGFGHIGFD
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
ALHYIVHHPQLADVPKILETPYVGEDKASKKAPYKWEIAMLRNGEFDPDLLNKIQNS
HHHHHHCCCCHHCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]