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The map label for this gene is ispG

Identifier: 226224042

GI number: 226224042

Start: 1458494

End: 1459600

Strand: Direct

Name: ispG

Synonym: Lm4b_01451

Alternate gene names: 226224042

Gene position: 1458494-1459600 (Clockwise)

Preceding gene: 226224041

Following gene: 226224043

Centisome position: 50.07

GC content: 40.02

Gene sequence:

>1107_bases
TTGAATGAAAGAATCTTTCGCGAAAACACTCGGCCAGTCCAAGTAGGTAATTTAACTATTGGTGGTAGTGAGGAATTAAC
TATCCAAAGCATGACTACTACAAAGACACATGATGTCGAAGCAACAGTAGCGGAAATTCACCGATTAGAAGAGGTGGGTT
GTCAGATTGTGCGAGTTGCTTGTCCTGATGAGCGTGCAGCCAATGCTCTTAGTGCCATCAAGAAAAGGATTCATATTCCT
CTTGTGGCAGATATTCATTTTGATTACCGACTAGCACTTAAAGCTATTGATGCAGGTGTTGACAAAATCCGGATTAATCC
TGGTAACATCGGTCGCCGTGATCGGGTGGAAAAAGTGGTTAATGCTGCTAAAGCAAAAAATATTCCAATCCGTATTGGGG
TTAATGCTGGTAGTTTAGAAAAGAAAATTATTCAAAAATACGGTTACCCTACTGCTGAAGGAATGGTAGAAAGTGCACTT
GATCATATTAAAATTCTCGAAGATTTAGATTTTTATGATATCATTGTTTCTTTGAAAGCTTCTGATGTGAATTTAGCAAT
TGAAGCTTATGATAAAGCTAGTCGCGCATTTAATTATCCTCTACATCTTGGAATTACAGAATCTGGTACGCAGTTTGCTG
GAGGAATAAAAAGTGCTGCTGGTTTAGGAGCGATACTCAGTTTGGGCATTGGAAATACATTACGGGTATCTTTGAGTGCT
GATCCTGTGGAAGAGATAAAAGTGGCCCGGGAGGTTTTAAAATCGTTTGGCCTTTCCTCGAATGCCGCCATGCTTATCTC
CTGCCCTACTTGCGGTCGAATAGAGATTGATTTGATTCGTATCGCTAATGAAGTGGAAAATTACATAGCAACGATTAAAG
CTCCGATTAAAGTAGCCGTGCTTGGCTGTGCGGTCAACGGTCCCGGAGAAGCTCGCGAAGCAGATATCGGAATTGCTGGT
TCAAATGGAGAAGGCCTTCTTTTTAGACATGGTAAAATCATCCGAAAAGTACCAGAAGCCATTATGGTAGAAGAACTTAA
GAAAGAAATTGATATTTTGGCAGAAGAATATTTTGAAAAGAAAACAGATTTGGAAAGCCTTAGATAA

Upstream 100 bases:

>100_bases
TTGCTCACGCAGGTGGCTTTTTTGTGGCATCATTCACGTGATTTTGTTTCATGAAAATGGTATGATAAAAAATAAAAGCT
AGTCTAAAGGAGCGGTCTCT

Downstream 100 bases:

>100_bases
TCGTATCGGGGTTTTTGTTTCAAGGGGGATTTTTGTTGGAAAAAGAAAATGAGCTTAGTTTTTATGATGGTGTTAGAGCC
TGCCTTCCTACCGTCCTTGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNERIFRENTRPVQVGNLTIGGSEELTIQSMTTTKTHDVEATVAEIHRLEEVGCQIVRVACPDERAANALSAIKKRIHIP
LVADIHFDYRLALKAIDAGVDKIRINPGNIGRRDRVEKVVNAAKAKNIPIRIGVNAGSLEKKIIQKYGYPTAEGMVESAL
DHIKILEDLDFYDIIVSLKASDVNLAIEAYDKASRAFNYPLHLGITESGTQFAGGIKSAAGLGAILSLGIGNTLRVSLSA
DPVEEIKVAREVLKSFGLSSNAAMLISCPTCGRIEIDLIRIANEVENYIATIKAPIKVAVLGCAVNGPGEAREADIGIAG
SNGEGLLFRHGKIIRKVPEAIMVEELKKEIDILAEEYFEKKTDLESLR

Sequences:

>Translated_368_residues
MNERIFRENTRPVQVGNLTIGGSEELTIQSMTTTKTHDVEATVAEIHRLEEVGCQIVRVACPDERAANALSAIKKRIHIP
LVADIHFDYRLALKAIDAGVDKIRINPGNIGRRDRVEKVVNAAKAKNIPIRIGVNAGSLEKKIIQKYGYPTAEGMVESAL
DHIKILEDLDFYDIIVSLKASDVNLAIEAYDKASRAFNYPLHLGITESGTQFAGGIKSAAGLGAILSLGIGNTLRVSLSA
DPVEEIKVAREVLKSFGLSSNAAMLISCPTCGRIEIDLIRIANEVENYIATIKAPIKVAVLGCAVNGPGEAREADIGIAG
SNGEGLLFRHGKIIRKVPEAIMVEELKKEIDILAEEYFEKKTDLESLR
>Mature_368_residues
MNERIFRENTRPVQVGNLTIGGSEELTIQSMTTTKTHDVEATVAEIHRLEEVGCQIVRVACPDERAANALSAIKKRIHIP
LVADIHFDYRLALKAIDAGVDKIRINPGNIGRRDRVEKVVNAAKAKNIPIRIGVNAGSLEKKIIQKYGYPTAEGMVESAL
DHIKILEDLDFYDIIVSLKASDVNLAIEAYDKASRAFNYPLHLGITESGTQFAGGIKSAAGLGAILSLGIGNTLRVSLSA
DPVEEIKVAREVLKSFGLSSNAAMLISCPTCGRIEIDLIRIANEVENYIATIKAPIKVAVLGCAVNGPGEAREADIGIAG
SNGEGLLFRHGKIIRKVPEAIMVEELKKEIDILAEEYFEKKTDLESLR

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=362, Percent_Identity=48.8950276243094, Blast_Score=341, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_LISMC (C1L2Z7)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758149.1
- GeneID:   7704465
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01451
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.20.20.70
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 39972; Mature: 39972

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERIFRENTRPVQVGNLTIGGSEELTIQSMTTTKTHDVEATVAEIHRLEEVGCQIVRVA
CCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPDERAANALSAIKKRIHIPLVADIHFDYRLALKAIDAGVDKIRINPGNIGRRDRVEKVV
CCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
NAAKAKNIPIRIGVNAGSLEKKIIQKYGYPTAEGMVESALDHIKILEDLDFYDIIVSLKA
HHHHCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYDKASRAFNYPLHLGITESGTQFAGGIKSAAGLGAILSLGIGNTLRVSLSA
CCCEEEEEEHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEECC
DPVEEIKVAREVLKSFGLSSNAAMLISCPTCGRIEIDLIRIANEVENYIATIKAPIKVAV
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEE
LGCAVNGPGEAREADIGIAGSNGEGLLFRHGKIIRKVPEAIMVEELKKEIDILAEEYFEK
EEEEECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTDLESLR
HCHHHHCC
>Mature Secondary Structure
MNERIFRENTRPVQVGNLTIGGSEELTIQSMTTTKTHDVEATVAEIHRLEEVGCQIVRVA
CCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPDERAANALSAIKKRIHIPLVADIHFDYRLALKAIDAGVDKIRINPGNIGRRDRVEKVV
CCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
NAAKAKNIPIRIGVNAGSLEKKIIQKYGYPTAEGMVESALDHIKILEDLDFYDIIVSLKA
HHHHCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYDKASRAFNYPLHLGITESGTQFAGGIKSAAGLGAILSLGIGNTLRVSLSA
CCCEEEEEEHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEECC
DPVEEIKVAREVLKSFGLSSNAAMLISCPTCGRIEIDLIRIANEVENYIATIKAPIKVAV
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEE
LGCAVNGPGEAREADIGIAGSNGEGLLFRHGKIIRKVPEAIMVEELKKEIDILAEEYFEK
EEEEECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTDLESLR
HCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA