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The map label for this gene is ymfH [H]

Identifier: 226223994

GI number: 226223994

Start: 1403779

End: 1405065

Strand: Direct

Name: ymfH [H]

Synonym: Lm4b_01402

Alternate gene names: 226223994

Gene position: 1403779-1405065 (Clockwise)

Preceding gene: 226223993

Following gene: 226223995

Centisome position: 48.2

GC content: 36.44

Gene sequence:

>1287_bases
ATGGAAAAAATCACATTTGAGCAAGTAAAAGAAGCAGTCTTTCATGAAAAAATGGCAAATGGCTTACAAGTGTATCTTCT
TCCAAAACAAGGTTTTAGTAAGACTTATGCTGTATTCACAACAAATTACGGTGCAATTGATAATAACTTCGTCCCAATTG
GTGAAACAGAATTCACGAAAGTACCAGATGGTATTGCCCATTTCTTAGAACATAAAATGTTTGAAAAAGAAGACGGCGAT
GTATTCTTCAAATTTGGCGAAAAAGGCGCGTTTACGAATGCATTTACCTCTTTCACAAAAACAGCTTATCTTTTCTCGAG
TACTTCTCGAGTGGAAGAAAACTTGGAAACGCTGATTGACTTTGTACAAGAGCCGTATTTCACAGAAGAAACTGTTGAAA
AAGAGAAAGGTATTATCGGCCAAGAAATCAGAATGTATGATGATGATCCTGATTTTCGCGCTTATTTTGGCGTAATCGAA
AATATGTATCATAATCATCCAGTAAAAATCGATATTGCTGGAACCGTTGAATCGATTGCCGAAATTAACAAAGATTTACT
GTATCTTTGCTATAACACATTCTATCACCCTAGCAACATGGTCCTTTTTGTTGTAGGGAATTTAGAACCAGAACAAATGA
TGGACCTGATTCGCGCCAATCAAGCGAAAAAAGAATTCGCTGAAGCTGCACCGATCAAACGTCATTTCCCTGAAGAACCA
AAAACAGTTGCAGTGAAAGAGCGTAAAATTCATTTCCCTGTTCAAATTGCTAAGAACCTTGTAGGGATTAAAGAAGATAT
TGGCTCTTTAGAAGGCCAAGCTGCTTTAAAACAAGAAATTATTGGAGATGTAGCCTTGGAAATGCTCTTTGGCACAACCT
CTGACACTTACTTGAAGTTATACAACGAAGGAATCATTGACGACACATTTGGCTATGATTACACCTTACAAGATAGCTTC
TCGTTCGTATTAGTTGGTGGAGATGCCAAAGATCCAGATAAACAAACCGAAAAAATCAAAGAAGCAATGAAAGAAGCTGC
CAAAAACGGCTTAAATGAATCTGATTTAGCATTAGTAAAACGCAAACGAATTGGTCAATTTTTGCGTTCGCTGAATTCTC
CGGAGTTTATTGCGAACCAATTTAGCCAATACGTTATGAAATCAGCTTCTTTATTCGATATTTTACCTTTAATGGAGGAA
GTTACCCTAGAAGAAGTAAATGCATTTGTTAAAAATTTGGATCAAGAAGAGCGAACAACAAGTTTCCAATTACTTCCAGA
ACAATAA

Upstream 100 bases:

>100_bases
GCGAAAATCAAACAAGCAACCAAAGAAGAAGTAGTTGCTGCTATCAATAAAATTAAACCAGATACCATTTATTTCTTAAG
TAAGGGAGGAGAAGAACTTC

Downstream 100 bases:

>100_bases
ACAAATTATGTCTTTTTTTAGTAGAAAAAATAGCATAAATTGCTGAGTCATGCTATCATAAAAGAAATAGACTGGAAAGG
GTTGCCTTTTTTTGGATAAA

Product: protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEKITFEQVKEAVFHEKMANGLQVYLLPKQGFSKTYAVFTTNYGAIDNNFVPIGETEFTKVPDGIAHFLEHKMFEKEDGD
VFFKFGEKGAFTNAFTSFTKTAYLFSSTSRVEENLETLIDFVQEPYFTEETVEKEKGIIGQEIRMYDDDPDFRAYFGVIE
NMYHNHPVKIDIAGTVESIAEINKDLLYLCYNTFYHPSNMVLFVVGNLEPEQMMDLIRANQAKKEFAEAAPIKRHFPEEP
KTVAVKERKIHFPVQIAKNLVGIKEDIGSLEGQAALKQEIIGDVALEMLFGTTSDTYLKLYNEGIIDDTFGYDYTLQDSF
SFVLVGGDAKDPDKQTEKIKEAMKEAAKNGLNESDLALVKRKRIGQFLRSLNSPEFIANQFSQYVMKSASLFDILPLMEE
VTLEEVNAFVKNLDQEERTTSFQLLPEQ

Sequences:

>Translated_428_residues
MEKITFEQVKEAVFHEKMANGLQVYLLPKQGFSKTYAVFTTNYGAIDNNFVPIGETEFTKVPDGIAHFLEHKMFEKEDGD
VFFKFGEKGAFTNAFTSFTKTAYLFSSTSRVEENLETLIDFVQEPYFTEETVEKEKGIIGQEIRMYDDDPDFRAYFGVIE
NMYHNHPVKIDIAGTVESIAEINKDLLYLCYNTFYHPSNMVLFVVGNLEPEQMMDLIRANQAKKEFAEAAPIKRHFPEEP
KTVAVKERKIHFPVQIAKNLVGIKEDIGSLEGQAALKQEIIGDVALEMLFGTTSDTYLKLYNEGIIDDTFGYDYTLQDSF
SFVLVGGDAKDPDKQTEKIKEAMKEAAKNGLNESDLALVKRKRIGQFLRSLNSPEFIANQFSQYVMKSASLFDILPLMEE
VTLEEVNAFVKNLDQEERTTSFQLLPEQ
>Mature_428_residues
MEKITFEQVKEAVFHEKMANGLQVYLLPKQGFSKTYAVFTTNYGAIDNNFVPIGETEFTKVPDGIAHFLEHKMFEKEDGD
VFFKFGEKGAFTNAFTSFTKTAYLFSSTSRVEENLETLIDFVQEPYFTEETVEKEKGIIGQEIRMYDDDPDFRAYFGVIE
NMYHNHPVKIDIAGTVESIAEINKDLLYLCYNTFYHPSNMVLFVVGNLEPEQMMDLIRANQAKKEFAEAAPIKRHFPEEP
KTVAVKERKIHFPVQIAKNLVGIKEDIGSLEGQAALKQEIIGDVALEMLFGTTSDTYLKLYNEGIIDDTFGYDYTLQDSF
SFVLVGGDAKDPDKQTEKIKEAMKEAAKNGLNESDLALVKRKRIGQFLRSLNSPEFIANQFSQYVMKSASLFDILPLMEE
VTLEEVNAFVKNLDQEERTTSFQLLPEQ

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Escherichia coli, GI1787770, Length=191, Percent_Identity=25.6544502617801, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 48834; Mature: 48834

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKITFEQVKEAVFHEKMANGLQVYLLPKQGFSKTYAVFTTNYGAIDNNFVPIGETEFTK
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCEECCCCCCHHC
VPDGIAHFLEHKMFEKEDGDVFFKFGEKGAFTNAFTSFTKTAYLFSSTSRVEENLETLID
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVQEPYFTEETVEKEKGIIGQEIRMYDDDPDFRAYFGVIENMYHNHPVKIDIAGTVESIA
HHHCCCCCHHHHHHHCCCCCCHHEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
EINKDLLYLCYNTFYHPSNMVLFVVGNLEPEQMMDLIRANQAKKEFAEAAPIKRHFPEEP
HHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
KTVAVKERKIHFPVQIAKNLVGIKEDIGSLEGQAALKQEIIGDVALEMLFGTTSDTYLKL
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YNEGIIDDTFGYDYTLQDSFSFVLVGGDAKDPDKQTEKIKEAMKEAAKNGLNESDLALVK
HCCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RKRIGQFLRSLNSPEFIANQFSQYVMKSASLFDILPLMEEVTLEEVNAFVKNLDQEERTT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
SFQLLPEQ
CEEECCCC
>Mature Secondary Structure
MEKITFEQVKEAVFHEKMANGLQVYLLPKQGFSKTYAVFTTNYGAIDNNFVPIGETEFTK
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCEECCCCCCHHC
VPDGIAHFLEHKMFEKEDGDVFFKFGEKGAFTNAFTSFTKTAYLFSSTSRVEENLETLID
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVQEPYFTEETVEKEKGIIGQEIRMYDDDPDFRAYFGVIENMYHNHPVKIDIAGTVESIA
HHHCCCCCHHHHHHHCCCCCCHHEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
EINKDLLYLCYNTFYHPSNMVLFVVGNLEPEQMMDLIRANQAKKEFAEAAPIKRHFPEEP
HHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
KTVAVKERKIHFPVQIAKNLVGIKEDIGSLEGQAALKQEIIGDVALEMLFGTTSDTYLKL
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YNEGIIDDTFGYDYTLQDSFSFVLVGGDAKDPDKQTEKIKEAMKEAAKNGLNESDLALVK
HCCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RKRIGQFLRSLNSPEFIANQFSQYVMKSASLFDILPLMEEVTLEEVNAFVKNLDQEERTT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
SFQLLPEQ
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]