BacMap

Definition	Listeria monocytogenes Clip81459, complete genome.
Accession	NC_012488
Length	2,912,690

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The map label for this gene is ymfI [H]

Identifier: 226223995

GI number: 226223995

Start: 1405157

End: 1405888

Strand: Direct

Name: ymfI [H]

Synonym: Lm4b_01403

Alternate gene names: 226223995

Gene position: 1405157-1405888 (Clockwise)

Preceding gene: 226223994

Following gene: 226223996

Centisome position: 48.24

GC content: 37.16

Gene sequence:

>732_bases
TTGGATAAAGAGATAAAATATGCTTTTGTAACAGGGGCTAGCGGGGAAATAGGTCAAGCAATTTGTTTGTCACTTGCGAA
AGCCGGTTGGAACTTGTATCTTCACTATTATCAAAATAAACAAGCAGTGGAAAAATTACTGCCACAATTACTAGCAGAAG
ATGTAGATGTTATACTGATTCAAGCTGATTTTGATGATTTAACAAGTTTAACAGAGATGGAGAAGCAAGTTTTTCAGTTA
GATGCCTTTATTCATGCAGCAGGGCACGCCCATTATGGTTTATTCCAGGATACTACAGATATAAATATTACTGAATTATG
GAATGTGCATATGTATATGCCAATGCGATTAATTCGCACTTTTATGCCCAAATTAATGAAAAGTAGCCAAGGAAGAATTA
TTTTCATTAGTTCTATATGGGGAGAAGTTGGTGCATCAATGGAAGTAGCGTATTCAACTGTAAAAGGTGCGCAAATAGCC
TTTTGCCGTGCGCTAAGTCAAGAGGTGGGACTTTCTGGAATAACAGTTAATGCAGTTACACCAGGGGTTGTAGAAACAAA
AATGATGGATCAGTTTTCGGACGAGGAGAAAGAAATTCTTCGTCAAGAAATCCCTTTAAATCGTTTTGCCCAACCAGAAG
AAATTGCAGAAATTGTAGCATTTTTGACAAGTAAAAAAGCAAGCTATATCACTGGAGAAGTTTTGCGCTTAAATGGCGGT
TGGCTTATGTAA

Upstream 100 bases:

>100_bases
GAACAATAAACAAATTATGTCTTTTTTTAGTAGAAAAAATAGCATAAATTGCTGAGTCATGCTATCATAAAAGAAATAGA
CTGGAAAGGGTTGCCTTTTT

Downstream 100 bases:

>100_bases
TTTTAGTAAAAAAATGGAAATTTGAAATTAGTAATGGTAGGTGTTTAATTTTGACAGAACTCGGTGATAAACTGAAACAA
GCTAGACGTGAAAAAGGACT

Product: 3-ketoacyl-acyl carrier protein reductase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MDKEIKYAFVTGASGEIGQAICLSLAKAGWNLYLHYYQNKQAVEKLLPQLLAEDVDVILIQADFDDLTSLTEMEKQVFQL
DAFIHAAGHAHYGLFQDTTDINITELWNVHMYMPMRLIRTFMPKLMKSSQGRIIFISSIWGEVGASMEVAYSTVKGAQIA
FCRALSQEVGLSGITVNAVTPGVVETKMMDQFSDEEKEILRQEIPLNRFAQPEEIAEIVAFLTSKKASYITGEVLRLNGG
WLM

Sequences:

>Translated_243_residues
MDKEIKYAFVTGASGEIGQAICLSLAKAGWNLYLHYYQNKQAVEKLLPQLLAEDVDVILIQADFDDLTSLTEMEKQVFQL
DAFIHAAGHAHYGLFQDTTDINITELWNVHMYMPMRLIRTFMPKLMKSSQGRIIFISSIWGEVGASMEVAYSTVKGAQIA
FCRALSQEVGLSGITVNAVTPGVVETKMMDQFSDEEKEILRQEIPLNRFAQPEEIAEIVAFLTSKKASYITGEVLRLNGG
WLM
>Mature_243_residues
MDKEIKYAFVTGASGEIGQAICLSLAKAGWNLYLHYYQNKQAVEKLLPQLLAEDVDVILIQADFDDLTSLTEMEKQVFQL
DAFIHAAGHAHYGLFQDTTDINITELWNVHMYMPMRLIRTFMPKLMKSSQGRIIFISSIWGEVGASMEVAYSTVKGAQIA
FCRALSQEVGLSGITVNAVTPGVVETKMMDQFSDEEKEILRQEIPLNRFAQPEEIAEIVAFLTSKKASYITGEVLRLNGG
WLM

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=239, Percent_Identity=31.3807531380753, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI66933014, Length=196, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=26.0162601626016, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI15277342, Length=250, Percent_Identity=27.2, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI59889578, Length=184, Percent_Identity=29.3478260869565, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI5031737, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI126723750, Length=245, Percent_Identity=23.6734693877551, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI20149619, Length=239, Percent_Identity=24.6861924686192, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4503301, Length=122, Percent_Identity=32.7868852459016, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI7706318, Length=191, Percent_Identity=24.0837696335079, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=33.6065573770492, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=32.03125, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI2367175, Length=247, Percent_Identity=31.9838056680162, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788459, Length=243, Percent_Identity=25.1028806584362, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367365, Length=240, Percent_Identity=25.8333333333333, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082160, Length=242, Percent_Identity=25.2066115702479, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789378, Length=246, Percent_Identity=26.0162601626016, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=24.4897959183673, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=25.4901960784314, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790717, Length=243, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786812, Length=251, Percent_Identity=26.2948207171315, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=23.4375, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=244, Percent_Identity=31.1475409836066, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=246, Percent_Identity=31.3008130081301, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71994600, Length=247, Percent_Identity=29.5546558704453, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=247, Percent_Identity=29.9595141700405, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI115534694, Length=243, Percent_Identity=30.8641975308642, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17555706, Length=253, Percent_Identity=28.4584980237154, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=246, Percent_Identity=25.609756097561, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508651, Length=246, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=257, Percent_Identity=26.0700389105058, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=265, Percent_Identity=28.3018867924528, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=28.7878787878788, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=29.3893129770992, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=259, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=28.1954887218045, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=270, Percent_Identity=23.7037037037037, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=27.5471698113208, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=257, Percent_Identity=26.0700389105058, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=257, Percent_Identity=26.0700389105058, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=260, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=192, Percent_Identity=25, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=246, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24639444, Length=245, Percent_Identity=29.7959183673469, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24643142, Length=239, Percent_Identity=31.7991631799163, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21355319, Length=246, Percent_Identity=28.4552845528455, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI23397609, Length=243, Percent_Identity=30.8641975308642, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358495, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571526, Length=249, Percent_Identity=28.9156626506024, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=28.4584980237154, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357041, Length=246, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24644337, Length=173, Percent_Identity=31.7919075144509, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 27252; Mature: 27252

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKEIKYAFVTGASGEIGQAICLSLAKAGWNLYLHYYQNKQAVEKLLPQLLAEDVDVILI
CCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEE
QADFDDLTSLTEMEKQVFQLDAFIHAAGHAHYGLFQDTTDINITELWNVHMYMPMRLIRT
EECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FMPKLMKSSQGRIIFISSIWGEVGASMEVAYSTVKGAQIAFCRALSQEVGLSGITVNAVT
HHHHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCC
PGVVETKMMDQFSDEEKEILRQEIPLNRFAQPEEIAEIVAFLTSKKASYITGEVLRLNGG
CCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCC
WLM
CCC
>Mature Secondary Structure
MDKEIKYAFVTGASGEIGQAICLSLAKAGWNLYLHYYQNKQAVEKLLPQLLAEDVDVILI
CCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEE
QADFDDLTSLTEMEKQVFQLDAFIHAAGHAHYGLFQDTTDINITELWNVHMYMPMRLIRT
EECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FMPKLMKSSQGRIIFISSIWGEVGASMEVAYSTVKGAQIAFCRALSQEVGLSGITVNAVT
HHHHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCC
PGVVETKMMDQFSDEEKEILRQEIPLNRFAQPEEIAEIVAFLTSKKASYITGEVLRLNGG
CCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCC
WLM
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]