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The map label for this gene is bfmBC [H]

Identifier: 226223972

GI number: 226223972

Start: 1378307

End: 1379734

Strand: Direct

Name: bfmBC [H]

Synonym: Lm4b_01380

Alternate gene names: 226223972

Gene position: 1378307-1379734 (Clockwise)

Preceding gene: 226223971

Following gene: 226223973

Centisome position: 47.32

GC content: 39.92

Gene sequence:

>1428_bases
GTGGCAACAGAATATGATGTCGTTATTCTTGGCGGAGGAACTGGCGGTTACGTCGCAGCAATTCAAGCAGCTAAGAATGG
CCAGAAAGTAGCCGTCGTTGAAAAAGGGAAAGTTGGTGGAACGTGTCTTCATCGTGGATGTATTCCAACGAAAGCGTTAT
TACGTTCAGCGGAAGTTCTGCAAACAGTAAAAAAAGCAAGTGAATTTGGTATTTCTGTAGAAGGAACTGCCGGAATCAAT
TTTTTACAAGCACAAGAAAGAAAACAAGCAATCGTAGATCAATTAGAAAAAGGTATTCACCAATTATTTAAACAAGGGAA
AATTGACTTGTTTGTTGGAACGGGAACTATTTTAGGACCATCTATTTTTTCACCAACAGCTGGAACAGTTTCAGTCGAAT
TTGAAGATGGCTCTGAAAATGAAATGCTCATTCCTAAAAATTTAATTATCGCGACAGGCTCCAAACCGCGCACATTAAAT
GGTTTAAGCATTGATGAAGAAAATGTTTTATCATCTGACGGTGCGCTAAACCTAGAAACTTTACCAAAATCAATTATTAT
CGTTGGCGGTGGAGTTATCGGGATGGAATGGGCTTCGATGATGCATGATTTTGGCGTAGAAGTTACCGTGCTAGAATATG
CAGATCGAATTTTGCCAACAGAAGATAAAGAAGTAGCTAAAGAATTAGCAAGACTTTATAAAAAGAAAAAATTAATCATG
CATACATCTGCTGAAGTTCAAGCAGCTAGTTATAAAAAAACGGATACTGGTGTGGAAATTAAAGCAATTATTAAAGGCGA
AGAGCAAACTTTCGCAGCAGACAAAATTCTCGTTTCTGTCGGTCGTTCAGCTAATACAGAAAATATCGGTTTGCAAAACA
CGGATATCGCGACCGAAAACGGCTTTATCCAAGTAAATGATTTTTATCAAACAAAAGAAAGCCATATCTACGCGATTGGT
GATTGCATTCCAACAATCCAACTTGCGCACGTTGCGATGGAAGAAGGAACGATTGCAGCCAACCATATTGCCGGAAAAGT
GGCTGAAAAACTTGACTACGACTTAGTTCCTCGCTGTATTTATACTTCTACAGAAATCGCAAGTGTCGGTATCACAGAAG
AACAAGCAAAAGAACGTGGTTATGAAGTGAAAAAAGGCAAATTTTTCTTCCGTGGTATCGGGAAAGCACTCGTTTACGGA
GAGTCAGATGGCTTCATTAAAATTATTGCAGATAAAAAAACAGACGATATCCTAGGCGTTAGCATGATTGGACCGCACGT
AACGGACATGATTAGCGAAGCCGCTTTAGCACAAGTTTTAAATGCAACGCCGTGGGAAGTGGGTAACACCATTCACCCGC
ACCCAACTTTATCAGAAAGTTTTAGAGAAGCTGCCCTTGCTGTGGATGGCAATGCAATTCACGGTTAA

Upstream 100 bases:

>100_bases
TAGAACCCGGAGAAGACGAATTAGAAGCATTAAATAGCGGTGTGCAACGTGTGCTCGCTGGTCTTGAGAAAGAAAAAGAG
TACTAAAAGGAGGAGATAAT

Downstream 100 bases:

>100_bases
TACAGCTTTAAAGGAGGAACAAAAATGACTTTAAAAGAAGCAGGTTTAACAGAAGATAAATTAATTAAAATGTATAAAAC
GATGCTAATGGCAAGAAGAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MATEYDVVILGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVLQTVKKASEFGISVEGTAGIN
FLQAQERKQAIVDQLEKGIHQLFKQGKIDLFVGTGTILGPSIFSPTAGTVSVEFEDGSENEMLIPKNLIIATGSKPRTLN
GLSIDEENVLSSDGALNLETLPKSIIIVGGGVIGMEWASMMHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKKLIM
HTSAEVQAASYKKTDTGVEIKAIIKGEEQTFAADKILVSVGRSANTENIGLQNTDIATENGFIQVNDFYQTKESHIYAIG
DCIPTIQLAHVAMEEGTIAANHIAGKVAEKLDYDLVPRCIYTSTEIASVGITEEQAKERGYEVKKGKFFFRGIGKALVYG
ESDGFIKIIADKKTDDILGVSMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLSESFREAALAVDGNAIHG

Sequences:

>Translated_475_residues
MATEYDVVILGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVLQTVKKASEFGISVEGTAGIN
FLQAQERKQAIVDQLEKGIHQLFKQGKIDLFVGTGTILGPSIFSPTAGTVSVEFEDGSENEMLIPKNLIIATGSKPRTLN
GLSIDEENVLSSDGALNLETLPKSIIIVGGGVIGMEWASMMHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKKLIM
HTSAEVQAASYKKTDTGVEIKAIIKGEEQTFAADKILVSVGRSANTENIGLQNTDIATENGFIQVNDFYQTKESHIYAIG
DCIPTIQLAHVAMEEGTIAANHIAGKVAEKLDYDLVPRCIYTSTEIASVGITEEQAKERGYEVKKGKFFFRGIGKALVYG
ESDGFIKIIADKKTDDILGVSMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLSESFREAALAVDGNAIHG
>Mature_474_residues
ATEYDVVILGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVLQTVKKASEFGISVEGTAGINF
LQAQERKQAIVDQLEKGIHQLFKQGKIDLFVGTGTILGPSIFSPTAGTVSVEFEDGSENEMLIPKNLIIATGSKPRTLNG
LSIDEENVLSSDGALNLETLPKSIIIVGGGVIGMEWASMMHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKKLIMH
TSAEVQAASYKKTDTGVEIKAIIKGEEQTFAADKILVSVGRSANTENIGLQNTDIATENGFIQVNDFYQTKESHIYAIGD
CIPTIQLAHVAMEEGTIAANHIAGKVAEKLDYDLVPRCIYTSTEIASVGITEEQAKERGYEVKKGKFFFRGIGKALVYGE
SDGFIKIIADKKTDDILGVSMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLSESFREAALAVDGNAIHG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=39.0191897654584, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=28.1449893390192, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=465, Percent_Identity=25.1612903225806, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519428, Length=465, Percent_Identity=25.1612903225806, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519426, Length=465, Percent_Identity=25.1612903225806, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277065, Length=465, Percent_Identity=25.1612903225806, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI148277071, Length=465, Percent_Identity=25.1612903225806, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI291045266, Length=485, Percent_Identity=27.0103092783505, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=24.1596638655462, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI291045268, Length=479, Percent_Identity=24.8434237995825, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1786307, Length=477, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=30.0420168067227, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=28.936170212766, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=25.6179775280899, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=41.6666666666667, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=24.8979591836735, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=465, Percent_Identity=26.6666666666667, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=465, Percent_Identity=26.6666666666667, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=498, Percent_Identity=24.4979919678715, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=39.375, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=28.099173553719, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=26.2605042016807, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=40.5117270788913, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=25.6147540983607, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24640551, Length=488, Percent_Identity=25.6147540983607, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=25.6147540983607, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=26.0780287474333, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50920; Mature: 50789

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEYDVVILGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVL
CCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
QTVKKASEFGISVEGTAGINFLQAQERKQAIVDQLEKGIHQLFKQGKIDLFVGTGTILGP
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECC
SIFSPTAGTVSVEFEDGSENEMLIPKNLIIATGSKPRTLNGLSIDEENVLSSDGALNLET
CCCCCCCCEEEEEECCCCCCCEECCCCEEEECCCCCCEECCCCCCHHHHCCCCCCEEHCC
LPKSIIIVGGGVIGMEWASMMHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKKLIM
CCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHEEE
HTSAEVQAASYKKTDTGVEIKAIIKGEEQTFAADKILVSVGRSANTENIGLQNTDIATEN
ECCCCEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCC
GFIQVNDFYQTKESHIYAIGDCIPTIQLAHVAMEEGTIAANHIAGKVAEKLDYDLVPRCI
CEEEEECCCCCCCCCEEEECCHHCHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHE
YTSTEIASVGITEEQAKERGYEVKKGKFFFRGIGKALVYGESDGFIKIIADKKTDDILGV
ECCCHHEECCCCHHHHHHCCCEECCCCEEEEECCCEEEEECCCCEEEEEECCCCCCEEEE
SMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLSESFREAALAVDGNAIHG
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
ATEYDVVILGGGTGGYVAAIQAAKNGQKVAVVEKGKVGGTCLHRGCIPTKALLRSAEVL
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
QTVKKASEFGISVEGTAGINFLQAQERKQAIVDQLEKGIHQLFKQGKIDLFVGTGTILGP
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECC
SIFSPTAGTVSVEFEDGSENEMLIPKNLIIATGSKPRTLNGLSIDEENVLSSDGALNLET
CCCCCCCCEEEEEECCCCCCCEECCCCEEEECCCCCCEECCCCCCHHHHCCCCCCEEHCC
LPKSIIIVGGGVIGMEWASMMHDFGVEVTVLEYADRILPTEDKEVAKELARLYKKKKLIM
CCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHEEE
HTSAEVQAASYKKTDTGVEIKAIIKGEEQTFAADKILVSVGRSANTENIGLQNTDIATEN
ECCCCEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCC
GFIQVNDFYQTKESHIYAIGDCIPTIQLAHVAMEEGTIAANHIAGKVAEKLDYDLVPRCI
CEEEEECCCCCCCCCEEEECCHHCHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHE
YTSTEIASVGITEEQAKERGYEVKKGKFFFRGIGKALVYGESDGFIKIIADKKTDDILGV
ECCCHHEECCCCHHHHHHCCCEECCCCEEEEECCCEEEEECCCCEEEEEECCCCCCEEEE
SMIGPHVTDMISEAALAQVLNATPWEVGNTIHPHPTLSESFREAALAVDGNAIHG
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]