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The map label for this gene is bfmBAA [H]

Identifier: 226223973

GI number: 226223973

Start: 1379759

End: 1380754

Strand: Direct

Name: bfmBAA [H]

Synonym: Lm4b_01381

Alternate gene names: 226223973

Gene position: 1379759-1380754 (Clockwise)

Preceding gene: 226223972

Following gene: 226223974

Centisome position: 47.37

GC content: 41.27

Gene sequence:

>996_bases
ATGACTTTAAAAGAAGCAGGTTTAACAGAAGATAAATTAATTAAAATGTATAAAACGATGCTAATGGCAAGAAGACTGGA
TGAACGTATGTGGTTGCTGAACCGTTCTGGGAAAATTCCTTTTACCATTTCTGGACAAGGACAAGAAACGGCACAAATTG
GCGCTGCGTTTGCCTTTGATTTAGATAAAGATTATGCATTACCATATTACCGTGATTTAGCAGTGGTGTTAGCATTCGGG
ATGACAGCGAAAGATATTATGTTATCCGCGTTCGCTAAAGCGGAAGATCCAAATTCTGGCGGACGTCAAATGCCAGCTCA
TTTTGGCCAAAAATCAAACCGAATCGTTACACAAAGTTCACCCGTAACAACCCAGTTCCCGCACGCAGCAGGAATTGGTC
TTGCAGCAAAAATGGCTGGTGACGAAATTGCGATTTATGCATCAACTGGGGAAGGATCTTCTAACCAAGGCGATTTCCAC
GAAGGAATCAACTTTGCATCTGTACATAAGTTGCCAGTTGTTTTCGTGATTCATAATAACCAATATGCTATTTCCGTTCC
AGCATCGAAACAATATGCCGCAGAAAAACTATCTGACCGAGCAATTGGTTACGGCATCCCTGGTGAACGTGTGGATGGAA
CGAATATGGGAGAAGTATATGCGGCATTTAAACGTGCAGCAGATCGTGCAAGAAACGGCGAGGGACCTACTTTAATTGAG
ACAGTCTCTTATCGCTTCACACCACACTCCTCCGATGATGACGATAGTAGTTATCGTTCCAGAGAAGAAGTAGATGAAGC
AAAAGGAAAAGATCCACTGAAAATTTTCCAAGCAGAATTACTCGAAGAAGGTTACTTAACAGAAGAAAAAATCGCTGAAA
TCGAAAAAAGTATTGCGAAAGAAGTTAACGAAGCAACTGATTACGCGGAAAGTGCAGCATACGCTGAACCAGAATCATCT
TTACTATATGTATATGATGAAGAAGCGAATAGCTGA

Upstream 100 bases:

>100_bases
TCACCCGCACCCAACTTTATCAGAAAGTTTTAGAGAAGCTGCCCTTGCTGTGGATGGCAATGCAATTCACGGTTAATACA
GCTTTAAAGGAGGAACAAAA

Downstream 100 bases:

>100_bases
TTAGGAGGGAATTTGAATGCCAGTCATTTCATATATTGATGCAATAACCATGGCGCTTAAAGAAGAAATGGAGCGCGATG
ATAAAGTATTTATTTTAGGA

Product: branched-chain alpha-keto acid dehydrogenase E1 subunit (2-oxoisovalerate dehydrogenase alpha subunit)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTLKEAGLTEDKLIKMYKTMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFDLDKDYALPYYRDLAVVLAFG
MTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSSPVTTQFPHAAGIGLAAKMAGDEIAIYASTGEGSSNQGDFH
EGINFASVHKLPVVFVIHNNQYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMGEVYAAFKRAADRARNGEGPTLIE
TVSYRFTPHSSDDDDSSYRSREEVDEAKGKDPLKIFQAELLEEGYLTEEKIAEIEKSIAKEVNEATDYAESAAYAEPESS
LLYVYDEEANS

Sequences:

>Translated_331_residues
MTLKEAGLTEDKLIKMYKTMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFDLDKDYALPYYRDLAVVLAFG
MTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSSPVTTQFPHAAGIGLAAKMAGDEIAIYASTGEGSSNQGDFH
EGINFASVHKLPVVFVIHNNQYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMGEVYAAFKRAADRARNGEGPTLIE
TVSYRFTPHSSDDDDSSYRSREEVDEAKGKDPLKIFQAELLEEGYLTEEKIAEIEKSIAKEVNEATDYAESAAYAEPESS
LLYVYDEEANS
>Mature_330_residues
TLKEAGLTEDKLIKMYKTMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFDLDKDYALPYYRDLAVVLAFGM
TAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSSPVTTQFPHAAGIGLAAKMAGDEIAIYASTGEGSSNQGDFHE
GINFASVHKLPVVFVIHNNQYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMGEVYAAFKRAADRARNGEGPTLIET
VSYRFTPHSSDDDDSSYRSREEVDEAKGKDPLKIFQAELLEEGYLTEEKIAEIEKSIAKEVNEATDYAESAAYAEPESSL
LYVYDEEANS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=323, Percent_Identity=36.5325077399381, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI258645172, Length=323, Percent_Identity=36.5325077399381, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4885543, Length=316, Percent_Identity=31.3291139240506, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI291084742, Length=316, Percent_Identity=29.746835443038, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4505685, Length=316, Percent_Identity=29.746835443038, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI291084744, Length=323, Percent_Identity=29.1021671826625, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291084757, Length=167, Percent_Identity=34.7305389221557, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI86563357, Length=327, Percent_Identity=36.697247706422, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI86563355, Length=327, Percent_Identity=36.697247706422, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17536047, Length=320, Percent_Identity=29.6875, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32564172, Length=320, Percent_Identity=29.6875, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6321026, Length=318, Percent_Identity=30.5031446540881, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355903, Length=295, Percent_Identity=30.5084745762712, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24639740, Length=316, Percent_Identity=32.2784810126582, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639744, Length=316, Percent_Identity=32.2784810126582, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28571106, Length=316, Percent_Identity=32.2784810126582, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639746, Length=268, Percent_Identity=31.7164179104478, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24639748, Length=306, Percent_Identity=29.4117647058824, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36320; Mature: 36189

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKEAGLTEDKLIKMYKTMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHEEEEEE
LDKDYALPYYRDLAVVLAFGMTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSS
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEECCC
PVTTQFPHAAGIGLAAKMAGDEIAIYASTGEGSSNQGDFHEGINFASVHKLPVVFVIHNN
CCCCCCCCHHCCCHHEEECCCEEEEEEECCCCCCCCCCHHHCCCEEEEECCEEEEEEECC
QYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMGEVYAAFKRAADRARNGEGPTLIE
EEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TVSYRFTPHSSDDDDSSYRSREEVDEAKGKDPLKIFQAELLEEGYLTEEKIAEIEKSIAK
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVNEATDYAESAAYAEPESSLLYVYDEEANS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TLKEAGLTEDKLIKMYKTMLMARRLDERMWLLNRSGKIPFTISGQGQETAQIGAAFAFD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHEEEEEE
LDKDYALPYYRDLAVVLAFGMTAKDIMLSAFAKAEDPNSGGRQMPAHFGQKSNRIVTQSS
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEECCC
PVTTQFPHAAGIGLAAKMAGDEIAIYASTGEGSSNQGDFHEGINFASVHKLPVVFVIHNN
CCCCCCCCHHCCCHHEEECCCEEEEEEECCCCCCCCCCHHHCCCEEEEECCEEEEEEECC
QYAISVPASKQYAAEKLSDRAIGYGIPGERVDGTNMGEVYAAFKRAADRARNGEGPTLIE
EEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TVSYRFTPHSSDDDDSSYRSREEVDEAKGKDPLKIFQAELLEEGYLTEEKIAEIEKSIAK
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVNEATDYAESAAYAEPESSLLYVYDEEANS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]