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The map label for this gene is nusA

Identifier: 226223923

GI number: 226223923

Start: 1335646

End: 1336764

Strand: Direct

Name: nusA

Synonym: Lm4b_01330

Alternate gene names: 226223923

Gene position: 1335646-1336764 (Clockwise)

Preceding gene: 226223922

Following gene: 226223924

Centisome position: 45.86

GC content: 39.86

Gene sequence:

>1119_bases
ATGAGCACAGAATTATTAGATGCTCTTCATGTGTTAGAACATGATAAAGGTATTTCAAGAGAGGTTTTAGTAGAAGCAAT
TGAAGCTGCTCTTACATCCGCGTATAAAAGAAACTTCAAAGATGCACAAAACGTACGTGTAGATTTAAATATGGAAAATG
GTTCTATCCGTGTTTTAGCTAGAAAAGAAGCAGTAGAACAAGTATTTGATTCTCGCCTTGAAATCTCTATGGAAGAAGCA
CACAAAGTGAATCCAGTATACAAACCAGGTGACGTCGTGGAGCTTGAAGTAACACCAAAAGATTTTGGCCGTATCGCAGC
TCAAACAGCGAAACAAGTTGTAACCCAACGTGTTCGTGAAGCGGAACGTGGCATCATTTACGATGAATTTATCGATCGCG
AAGATGATATTATGACTGGTATTGTTGAACGTCAAGATTCTCGTTTTATCTATGTAAACCTTGGTAAAATCGAAGCAATT
TTGTCCCAAAACGAGCAAATGCCAAACGAAACTTATCATGCACATGACCGCATCAAAGTATATTTAACAAAAGTAGAAAA
GACGACAAAAGGGCCGCAAATTTTTGTATCCCGTACACACCCTGGCTTACTGAAACGTCTTTTTGAAATGGAAGTACCTG
AAATCTATGACGGCGTTGTAGAAATCAAGTCTGTTGCACGCGAAGCAGGAGATCGTTCTAAAATCTCTGTTTACACTGCA
AATGAAGAAGTAGATCCAGTTGGCGCATGTGTAGGACCAAAAGGCGCACGTGTCCAAACGATCGTCAATGAACTTAAAGG
CGAAAAAATCGATATCGTTGAATGGTCAGAAGATCCTTTCACATTCGTAGCCAATGCACTTAGCCCTTCCAAAGTGTTAG
ATGTTATTGTGAACGAAGCAGACCAAGCAACAACGGTAATCGTACCAGACTATCAGTTATCACTAGCAATTGGTAAACGT
GGTCAAAATGCCCGTTTAGCAGCAAAACTAACTGGTTGGAAAATTGATATTAAAAGTGAAACAGTTGCAACTGAACTAGG
CATCTATCCTCGTAATGACGTAGAAGCGCCAGAAGTGGAAGATGCAGAGAGCGAAACTTATACAGAAGATGAAGAGTAA

Upstream 100 bases:

>100_bases
AAACACGCAAAATCACTTGTGAAATTCCTAAAGACAAAGTAGCCAAAGCAAGACTCGCAATTCAATTTTAAAAAGCAAAG
AATAAGGAGCTGAATAAAAA

Downstream 100 bases:

>100_bases
AAAGGAGAATTATCATGCGTAATAAAAAAATCCCCCTTCGAAAATGTATTATTACCGGTGAACGCTTGCCAAAAGGCGAA
CTTCTTCGTATTGCGTATTC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSTELLDALHVLEHDKGISREVLVEAIEAALTSAYKRNFKDAQNVRVDLNMENGSIRVLARKEAVEQVFDSRLEISMEEA
HKVNPVYKPGDVVELEVTPKDFGRIAAQTAKQVVTQRVREAERGIIYDEFIDREDDIMTGIVERQDSRFIYVNLGKIEAI
LSQNEQMPNETYHAHDRIKVYLTKVEKTTKGPQIFVSRTHPGLLKRLFEMEVPEIYDGVVEIKSVAREAGDRSKISVYTA
NEEVDPVGACVGPKGARVQTIVNELKGEKIDIVEWSEDPFTFVANALSPSKVLDVIVNEADQATTVIVPDYQLSLAIGKR
GQNARLAAKLTGWKIDIKSETVATELGIYPRNDVEAPEVEDAESETYTEDEE

Sequences:

>Translated_372_residues
MSTELLDALHVLEHDKGISREVLVEAIEAALTSAYKRNFKDAQNVRVDLNMENGSIRVLARKEAVEQVFDSRLEISMEEA
HKVNPVYKPGDVVELEVTPKDFGRIAAQTAKQVVTQRVREAERGIIYDEFIDREDDIMTGIVERQDSRFIYVNLGKIEAI
LSQNEQMPNETYHAHDRIKVYLTKVEKTTKGPQIFVSRTHPGLLKRLFEMEVPEIYDGVVEIKSVAREAGDRSKISVYTA
NEEVDPVGACVGPKGARVQTIVNELKGEKIDIVEWSEDPFTFVANALSPSKVLDVIVNEADQATTVIVPDYQLSLAIGKR
GQNARLAAKLTGWKIDIKSETVATELGIYPRNDVEAPEVEDAESETYTEDEE
>Mature_371_residues
STELLDALHVLEHDKGISREVLVEAIEAALTSAYKRNFKDAQNVRVDLNMENGSIRVLARKEAVEQVFDSRLEISMEEAH
KVNPVYKPGDVVELEVTPKDFGRIAAQTAKQVVTQRVREAERGIIYDEFIDREDDIMTGIVERQDSRFIYVNLGKIEAIL
SQNEQMPNETYHAHDRIKVYLTKVEKTTKGPQIFVSRTHPGLLKRLFEMEVPEIYDGVVEIKSVAREAGDRSKISVYTAN
EEVDPVGACVGPKGARVQTIVNELKGEKIDIVEWSEDPFTFVANALSPSKVLDVIVNEADQATTVIVPDYQLSLAIGKRG
QNARLAAKLTGWKIDIKSETVATELGIYPRNDVEAPEVEDAESETYTEDEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=340, Percent_Identity=39.1176470588235, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 41731; Mature: 41599

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELLDALHVLEHDKGISREVLVEAIEAALTSAYKRNFKDAQNVRVDLNMENGSIRVLA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEE
RKEAVEQVFDSRLEISMEEAHKVNPVYKPGDVVELEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHHHHHHCEEHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGIIYDEFIDREDDIMTGIVERQDSRFIYVNLGKIEAILSQNEQMPNETYHAHDRIKV
HHCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCHHCCCCEEEE
YLTKVEKTTKGPQIFVSRTHPGLLKRLFEMEVPEIYDGVVEIKSVAREAGDRSKISVYTA
EEEEHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
NEEVDPVGACVGPKGARVQTIVNELKGEKIDIVEWSEDPFTFVANALSPSKVLDVIVNEA
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHCCC
DQATTVIVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSETVATELGIYPRNDVEAPEVE
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCC
DAESETYTEDEE
CCCCCCCCCCCC
>Mature Secondary Structure 
STELLDALHVLEHDKGISREVLVEAIEAALTSAYKRNFKDAQNVRVDLNMENGSIRVLA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEE
RKEAVEQVFDSRLEISMEEAHKVNPVYKPGDVVELEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHHHHHHCEEHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGIIYDEFIDREDDIMTGIVERQDSRFIYVNLGKIEAILSQNEQMPNETYHAHDRIKV
HHCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCHHCCCCEEEE
YLTKVEKTTKGPQIFVSRTHPGLLKRLFEMEVPEIYDGVVEIKSVAREAGDRSKISVYTA
EEEEHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
NEEVDPVGACVGPKGARVQTIVNELKGEKIDIVEWSEDPFTFVANALSPSKVLDVIVNEA
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHCCC
DQATTVIVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSETVATELGIYPRNDVEAPEVE
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCC
DAESETYTEDEE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]