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The map label for this gene is parE

Identifier: 226223889

GI number: 226223889

Start: 1294672

End: 1296639

Strand: Direct

Name: parE

Synonym: Lm4b_01296

Alternate gene names: 226223889

Gene position: 1294672-1296639 (Clockwise)

Preceding gene: 226223888

Following gene: 226223890

Centisome position: 44.45

GC content: 42.33

Gene sequence:

>1968_bases
ATGAATCGGAATGAGTATAATGATGATTCTATTCAGGTGCTTGAAGGACTGGAAGCGGTCCGGAAACGCCCAGCGATGTA
TATCGGTTCAACAGATGTACGCGGGCTGCACCATTTAGTATATGAAATAGTGGACAACTCGGTCGATGAGACCCTTGCAG
GTTTTGGTAAGAAAATTGTTGTGACACTTCACGTGGATGGCAGTGTTAGTGTTAGCGATGAAGGTCGTGGGATGCCTGTT
GGGATGCACAAAACAGGTAAGTCTACGGTAGAAGTTATTTTAACTGTTCTTCACGCCGGCGGTAAGTTCGGCCAAGAAGG
CGGATATAAAACAAGTGGTGGGCTTCACGGTGTTGGTTCATCCGTTGTAAATGCGCTCTCTGAATGGTTAGTAGTTACAA
TTAATCGTGACGGCGCAACCTACCAACAAAAATTTAAAGACGGCGGAAAGCCAGATGGAACACTGAAAAAAATCGGAAAA
TCAAAAGCTACTGGGACAACGATTCGCTTTAAGCCAGATCCAGCAATTTTTCCAACGACTTCATTTAACTATGAAACCTT
ATCAGAACGTCTAAGAGAATCAGCGTTCCTTTTAAAAGGGATGTTAATTGAATTAATTGATGAACGAGTTGGCATGGCAG
AGGCTTTCCACTTTGAAGAAGGTGTGAAAAACTTTGTTGAATATATTAATGAAGGTAAAGATGTGCTTCATCCTGTAGCT
AGCTTTGAAGGGGAAAATGCGACCATTGAAGTAGAAATGGCGTTCCAATTCAATGATGGTTATTCTGAAAATATCTTGAG
TTTCGTTAATAACGTTCGAACTCGCGGAGCAGGCTCGCATGAATCTGGAATGAAAGCTGCCATGACGCGGATTTTCAACG
ACTATGCTCGTCGAGTGAATCTTTTGAAAGAAAAAGATAAAAATCTCGAAGGTAGCGATATTCGTGAAGGTCTTTCAGCT
GTACTTTCTATTCGTGTGCCAGAAAAAATCCTTCAATTCGAAGGTCAAACGAAAGAAAAACTAGGGACGCAGGAAGCTCG
TCAGGCGGTTGATGCAGTTGTAGCAGAACATTTAGCTTACTTCCTTGCCGAAAACCCAGAAACAAGCTCTCTTCTAGTTA
AAAAGGCTGTTAAAGCCCGCGAAGCAAGAGAAGCTGCCAGAAAAGCGCGCGAAGAAACACGTAACGGCAAGAAAAAGAAA
CGTTCCGAAACACTTCTTTCTGGAAAGTTAACGCCGGCACAATCGCGAAATCCTAATAAAAATGAACTTTACCTAGTCGA
AGGTGACTCGGCGGGGGGATCAGCTAAACAAGGTCGTGACCGTCGTTTCCAAGCAATTTTGCCACTTCGAGGAAAAGTAA
TCAATACAGAAAAAGCCAAACTACAAGATATCTTAAAAAATGAAGAAATTAGCACGATTATCCATACAGTAGGTGCAGGC
GTTGGTACCGAATTTGATGTCGAGGATTGTAACTATGACAAAGTAGTTATCATGACCGATGCCGATACCGATGGCGCGCA
CATTCAAGTATTACTATTAACCTTTTTCTATCGTTATATGCGCCCACTAGTGGAAGCAGGTAAAGTATTTATTGCTTTAC
CCCCACTTTACAAAGTAAGCCGTGGTTCTGGTAAAAAAGAAGTAATCGAATATGCTTGGACAGATGAAGAATTAGACTCT
GCCATCCAAAAAATTGGTAAAGGCTACATGATTCAACGATACAAAGGTCTTGGTGAGATGAATGCTGACCAACTTTGGGA
AACAACGATGAATCCTGATACGCGCACGTTAATTCGTGTACGGGTAGATGATTCTGCACGCGCTGAACGCCGCGTGGCCA
CACTGATGGGCGACAAAGTAGAACCAAGACGACAATGGATTGAAAAGCATGTCGAGTTTTCTATGGAAGATAGCCAAAAT
ATTTTAGAAAATGAAAACATGATGGTTGAGGAGGCGAAAGACATTTGA

Upstream 100 bases:

>100_bases
ACTTTATTAATCAGCTAGTTAATGCTATTCTGATAAGGTGGTTTTAAGCGAATAACAACGTGAAAGTTGTAGATTGACGG
AATGAAGGGAGTTTTGAAAT

Downstream 100 bases:

>100_bases
GTAATCCAGAACAACATATCCAAGACTTAGCGCTAGAAGAAGTTATGGGCGACCGTTTTGGTAGATATAGTAAATATATT
ATTCAAGAACGTGCGCTTCC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MNRNEYNDDSIQVLEGLEAVRKRPAMYIGSTDVRGLHHLVYEIVDNSVDETLAGFGKKIVVTLHVDGSVSVSDEGRGMPV
GMHKTGKSTVEVILTVLHAGGKFGQEGGYKTSGGLHGVGSSVVNALSEWLVVTINRDGATYQQKFKDGGKPDGTLKKIGK
SKATGTTIRFKPDPAIFPTTSFNYETLSERLRESAFLLKGMLIELIDERVGMAEAFHFEEGVKNFVEYINEGKDVLHPVA
SFEGENATIEVEMAFQFNDGYSENILSFVNNVRTRGAGSHESGMKAAMTRIFNDYARRVNLLKEKDKNLEGSDIREGLSA
VLSIRVPEKILQFEGQTKEKLGTQEARQAVDAVVAEHLAYFLAENPETSSLLVKKAVKAREAREAARKAREETRNGKKKK
RSETLLSGKLTPAQSRNPNKNELYLVEGDSAGGSAKQGRDRRFQAILPLRGKVINTEKAKLQDILKNEEISTIIHTVGAG
VGTEFDVEDCNYDKVVIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKVFIALPPLYKVSRGSGKKEVIEYAWTDEELDS
AIQKIGKGYMIQRYKGLGEMNADQLWETTMNPDTRTLIRVRVDDSARAERRVATLMGDKVEPRRQWIEKHVEFSMEDSQN
ILENENMMVEEAKDI

Sequences:

>Translated_655_residues
MNRNEYNDDSIQVLEGLEAVRKRPAMYIGSTDVRGLHHLVYEIVDNSVDETLAGFGKKIVVTLHVDGSVSVSDEGRGMPV
GMHKTGKSTVEVILTVLHAGGKFGQEGGYKTSGGLHGVGSSVVNALSEWLVVTINRDGATYQQKFKDGGKPDGTLKKIGK
SKATGTTIRFKPDPAIFPTTSFNYETLSERLRESAFLLKGMLIELIDERVGMAEAFHFEEGVKNFVEYINEGKDVLHPVA
SFEGENATIEVEMAFQFNDGYSENILSFVNNVRTRGAGSHESGMKAAMTRIFNDYARRVNLLKEKDKNLEGSDIREGLSA
VLSIRVPEKILQFEGQTKEKLGTQEARQAVDAVVAEHLAYFLAENPETSSLLVKKAVKAREAREAARKAREETRNGKKKK
RSETLLSGKLTPAQSRNPNKNELYLVEGDSAGGSAKQGRDRRFQAILPLRGKVINTEKAKLQDILKNEEISTIIHTVGAG
VGTEFDVEDCNYDKVVIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKVFIALPPLYKVSRGSGKKEVIEYAWTDEELDS
AIQKIGKGYMIQRYKGLGEMNADQLWETTMNPDTRTLIRVRVDDSARAERRVATLMGDKVEPRRQWIEKHVEFSMEDSQN
ILENENMMVEEAKDI
>Mature_655_residues
MNRNEYNDDSIQVLEGLEAVRKRPAMYIGSTDVRGLHHLVYEIVDNSVDETLAGFGKKIVVTLHVDGSVSVSDEGRGMPV
GMHKTGKSTVEVILTVLHAGGKFGQEGGYKTSGGLHGVGSSVVNALSEWLVVTINRDGATYQQKFKDGGKPDGTLKKIGK
SKATGTTIRFKPDPAIFPTTSFNYETLSERLRESAFLLKGMLIELIDERVGMAEAFHFEEGVKNFVEYINEGKDVLHPVA
SFEGENATIEVEMAFQFNDGYSENILSFVNNVRTRGAGSHESGMKAAMTRIFNDYARRVNLLKEKDKNLEGSDIREGLSA
VLSIRVPEKILQFEGQTKEKLGTQEARQAVDAVVAEHLAYFLAENPETSSLLVKKAVKAREAREAARKAREETRNGKKKK
RSETLLSGKLTPAQSRNPNKNELYLVEGDSAGGSAKQGRDRRFQAILPLRGKVINTEKAKLQDILKNEEISTIIHTVGAG
VGTEFDVEDCNYDKVVIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKVFIALPPLYKVSRGSGKKEVIEYAWTDEELDS
AIQKIGKGYMIQRYKGLGEMNADQLWETTMNPDTRTLIRVRVDDSARAERRVATLMGDKVEPRRQWIEKHVEFSMEDSQN
ILENENMMVEEAKDI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=616, Percent_Identity=24.512987012987, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI19913408, Length=683, Percent_Identity=23.1332357247438, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=50.445632798574, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1789408, Length=640, Percent_Identity=37.8125, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535065, Length=670, Percent_Identity=24.7761194029851, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=615, Percent_Identity=25.2032520325203, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=204, Percent_Identity=29.9019607843137, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=683, Percent_Identity=25.0366032210835, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136538, Length=678, Percent_Identity=25.6637168141593, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 72986; Mature: 72986

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNEYNDDSIQVLEGLEAVRKRPAMYIGSTDVRGLHHLVYEIVDNSVDETLAGFGKKIV
CCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHCCCCEEE
VTLHVDGSVSVSDEGRGMPVGMHKTGKSTVEVILTVLHAGGKFGQEGGYKTSGGLHGVGS
EEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
SVVNALSEWLVVTINRDGATYQQKFKDGGKPDGTLKKIGKSKATGTTIRFKPDPAIFPTT
HHHHHHHCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCC
SFNYETLSERLRESAFLLKGMLIELIDERVGMAEAFHFEEGVKNFVEYINEGKDVLHPVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SFEGENATIEVEMAFQFNDGYSENILSFVNNVRTRGAGSHESGMKAAMTRIFNDYARRVN
HCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLKEKDKNLEGSDIREGLSAVLSIRVPEKILQFEGQTKEKLGTQEARQAVDAVVAEHLAY
HHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHH
FLAENPETSSLLVKKAVKAREAREAARKAREETRNGKKKKRSETLLSGKLTPAQSRNPNK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
NELYLVEGDSAGGSAKQGRDRRFQAILPLRGKVINTEKAKLQDILKNEEISTIIHTVGAG
CEEEEEECCCCCCCCCCCCCCHHHEECCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCC
VGTEFDVEDCNYDKVVIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKVFIALPPLYKVS
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
RGSGKKEVIEYAWTDEELDSAIQKIGKGYMIQRYKGLGEMNADQLWETTMNPDTRTLIRV
CCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHCCCCCCHHHHHHHCCCCCCEEEEEE
RVDDSARAERRVATLMGDKVEPRRQWIEKHVEFSMEDSQNILENENMMVEEAKDI
EECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCEEHHHHCCC
>Mature Secondary Structure
MNRNEYNDDSIQVLEGLEAVRKRPAMYIGSTDVRGLHHLVYEIVDNSVDETLAGFGKKIV
CCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHCCCCEEE
VTLHVDGSVSVSDEGRGMPVGMHKTGKSTVEVILTVLHAGGKFGQEGGYKTSGGLHGVGS
EEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
SVVNALSEWLVVTINRDGATYQQKFKDGGKPDGTLKKIGKSKATGTTIRFKPDPAIFPTT
HHHHHHHCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCC
SFNYETLSERLRESAFLLKGMLIELIDERVGMAEAFHFEEGVKNFVEYINEGKDVLHPVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SFEGENATIEVEMAFQFNDGYSENILSFVNNVRTRGAGSHESGMKAAMTRIFNDYARRVN
HCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LLKEKDKNLEGSDIREGLSAVLSIRVPEKILQFEGQTKEKLGTQEARQAVDAVVAEHLAY
HHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHH
FLAENPETSSLLVKKAVKAREAREAARKAREETRNGKKKKRSETLLSGKLTPAQSRNPNK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
NELYLVEGDSAGGSAKQGRDRRFQAILPLRGKVINTEKAKLQDILKNEEISTIIHTVGAG
CEEEEEECCCCCCCCCCCCCCHHHEECCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCC
VGTEFDVEDCNYDKVVIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKVFIALPPLYKVS
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
RGSGKKEVIEYAWTDEELDSAIQKIGKGYMIQRYKGLGEMNADQLWETTMNPDTRTLIRV
CCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHCCCCCCHHHHHHHCCCCCCEEEEEE
RVDDSARAERRVATLMGDKVEPRRQWIEKHVEFSMEDSQNILENENMMVEEAKDI
EECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]