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The map label for this gene is qor [C]

Identifier: 226223242

GI number: 226223242

Start: 656682

End: 657623

Strand: Direct

Name: qor [C]

Synonym: Lm4b_00639

Alternate gene names: 226223242

Gene position: 656682-657623 (Clockwise)

Preceding gene: 226223241

Following gene: 226223243

Centisome position: 22.55

GC content: 40.13

Gene sequence:

>942_bases
ATGAAAGCAGTTGTAATTGAAAATTATGGTGGCAAAGAAGAATTAAAAGAAAAAGAAGTAGCTATGCCAAAAGCGGGTAA
AAATCAAGTGATTGTCAAAGAAGCAGCGACATCAATTAACCCAATTGATTGGAAACTTCGTGAAGGATACTTAAAACAAA
TGATGGACTGGGAATTCCCGATTATTTTAGGATGGGATGTAGCGGGTGTTATTTCCGAAGTTGGAGAAGGCGTAACAGAT
TGGAAAGTCGGCGATGAAGTATTCGCACGCCCTGAGACAACGCGTTTTGGTACGTATGCTGAATATACAGCGGTAGATGA
TCACTTGCTTGCACCACTTCCAGAAGGAATTAGTTTTGACGAAGCAGCATCGATTCCACTTGCAGGCTTAACTGCTTGGC
AAGCATTGTTCGATCACGCGAAATTACAAAAAGGTGAAAAAGTCTTGATTCATGCTGGTGCTGGTGGTGTTGGGACGTTG
GCGATTCAGCTTGCCAAACATGCAGGAGCAGAAGTAATCACAACAGCTAGTGCGAAAAACCATGAATTACTAAAATCTCT
TGGCGCAGACCAAGTCATTGATTATAAAGAAGTTAATTTTAAAGATGTTCTTTCTGATATTGATGTGGTGTTCGATACAA
TGGGTGGTCAAATCGAAACAGATAGTTATGACGTATTAAAAGAAGGCACAGGGCGTTTAGTTAGTATCGTCGGTATTTCA
AATGAAGAACGTGCGAAAGAAAAAAATGTAACAGCAACTGGAATTTGGTTGCAACCAAACGGCGAACAGCTGAAAGAACT
TGGCAAATTGCTTGCTAATAAAACGGTTAAACCAATCGTCGGCGCAACTTTCCCATTCTCTGAAAAAGGTGTTTTTGATG
CACATGCATTAAGCGAAACGCACCACGCGGTTGGAAAAATAGTCATTTCATTTAATAAATAA

Upstream 100 bases:

>100_bases
ACGGGGCGCTAGAAGCCTTTTCAGAAGAAGAAGTTAGTGCGTTACTTAAATCAGTCACAAAAATGATGAATAATGTAGAG
AAAATGGAGGAGAAGTAAAA

Downstream 100 bases:

>100_bases
GATTTTCGGAAAGCTAGATGCTATCATCTAGCTTTTTTTGTTGTTTGCTGGAATAAGTTAGATGGGCTATAATAGCAAAA
AAGGTGGTGTCTAGATGGAA

Product: oxidoreductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFPIILGWDVAGVISEVGEGVTD
WKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFDEAASIPLAGLTAWQALFDHAKLQKGEKVLIHAGAGGVGTL
AIQLAKHAGAEVITTASAKNHELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGQIETDSYDVLKEGTGRLVSIVGIS
NEERAKEKNVTATGIWLQPNGEQLKELGKLLANKTVKPIVGATFPFSEKGVFDAHALSETHHAVGKIVISFNK

Sequences:

>Translated_313_residues
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFPIILGWDVAGVISEVGEGVTD
WKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFDEAASIPLAGLTAWQALFDHAKLQKGEKVLIHAGAGGVGTL
AIQLAKHAGAEVITTASAKNHELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGQIETDSYDVLKEGTGRLVSIVGIS
NEERAKEKNVTATGIWLQPNGEQLKELGKLLANKTVKPIVGATFPFSEKGVFDAHALSETHHAVGKIVISFNK
>Mature_313_residues
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFPIILGWDVAGVISEVGEGVTD
WKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFDEAASIPLAGLTAWQALFDHAKLQKGEKVLIHAGAGGVGTL
AIQLAKHAGAEVITTASAKNHELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGQIETDSYDVLKEGTGRLVSIVGIS
NEERAKEKNVTATGIWLQPNGEQLKELGKLLANKTVKPIVGATFPFSEKGVFDAHALSETHHAVGKIVISFNK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=354, Percent_Identity=33.0508474576271, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI194239674, Length=314, Percent_Identity=30.5732484076433, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI13236495, Length=314, Percent_Identity=30.5732484076433, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI194239676, Length=301, Percent_Identity=32.890365448505, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI22538446, Length=350, Percent_Identity=30, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI22538444, Length=350, Percent_Identity=30, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI18379349, Length=260, Percent_Identity=33.4615384615385, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI24308257, Length=235, Percent_Identity=28.936170212766, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI28557745, Length=235, Percent_Identity=30.2127659574468, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI90669511, Length=183, Percent_Identity=33.879781420765, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI67078404, Length=276, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI67078406, Length=254, Percent_Identity=27.5590551181102, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790485, Length=236, Percent_Identity=34.3220338983051, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17507255, Length=201, Percent_Identity=37.3134328358209, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71987554, Length=349, Percent_Identity=29.512893982808, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17562876, Length=310, Percent_Identity=28.0645161290323, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536829, Length=270, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988145, Length=222, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556000, Length=254, Percent_Identity=27.9527559055118, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=336, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324195, Length=301, Percent_Identity=28.9036544850498, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319945, Length=256, Percent_Identity=30.46875, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=245, Percent_Identity=29.3877551020408, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=236, Percent_Identity=30.0847457627119, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323493, Length=256, Percent_Identity=29.296875, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319401, Length=159, Percent_Identity=33.9622641509434, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24581339, Length=362, Percent_Identity=29.2817679558011, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24581341, Length=362, Percent_Identity=28.7292817679558, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550423, Length=241, Percent_Identity=26.5560165975104, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920632, Length=181, Percent_Identity=29.2817679558011, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI221330659, Length=181, Percent_Identity=29.2817679558011, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 33940; Mature: 33940

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFP
CCEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCC
IILGWDVAGVISEVGEGVTDWKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFD
EEECCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCC
EAASIPLAGLTAWQALFDHAKLQKGEKVLIHAGAGGVGTLAIQLAKHAGAEVITTASAKN
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCC
HELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGQIETDSYDVLKEGTGRLVSIVGIS
HHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCCEEEEEECC
NEERAKEKNVTATGIWLQPNGEQLKELGKLLANKTVKPIVGATFPFSEKGVFDAHALSET
CHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHH
HHAVGKIVISFNK
HHCEEEEEEEECC
>Mature Secondary Structure
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFP
CCEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCC
IILGWDVAGVISEVGEGVTDWKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFD
EEECCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCC
EAASIPLAGLTAWQALFDHAKLQKGEKVLIHAGAGGVGTLAIQLAKHAGAEVITTASAKN
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCC
HELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGQIETDSYDVLKEGTGRLVSIVGIS
HHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCCEEEEEECC
NEERAKEKNVTATGIWLQPNGEQLKELGKLLANKTVKPIVGATFPFSEKGVFDAHALSET
CHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHH
HHAVGKIVISFNK
HHCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]