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The map label for this gene is ywcJ [H]

Identifier: 226223222

GI number: 226223222

Start: 638119

End: 638919

Strand: Direct

Name: ywcJ [H]

Synonym: Lm4b_00619

Alternate gene names: 226223222

Gene position: 638119-638919 (Clockwise)

Preceding gene: 226223221

Following gene: 226223225

Centisome position: 21.91

GC content: 37.08

Gene sequence:

>801_bases
TTGGATGTGGAAAGTAGTCCATTAATGCAGAAAATTGATTATAGTACGCGCAAAAAAATTGATTTAGTAAATAATAGTAT
TCTTCGTTATATCGTGCGCGCGATGTTAGCATGTTTATTCTTGACACTAGGGACTGCGGTCGCTGTAATGATTGGTGATA
AAGTTGATCATTTCGCTCCAGGACTTGGTAAAATCACGTATGCATTTATGTTTAGTTGGTCGCTCGTTATGATTATTTAT
ATGAATGCTGAGCTTGGTACTTCTAACATGATGTATATGACAACTGGTGTTTATCAAAAAATTGTTAAACCAGGAAAAGC
ATTACAAATTTTGCTTTTATGTATTGTTTGTAATTTATTGGGTGGAATTCTTGCCGGGTACTTAGTTTCCCTAACATCCG
TTTTCCATAATTTACCTGCTGACCATTTCTTATTTACTGCTGTTAGCGGTAAGCTTGAAAAAGCGCCACTACAAATTTTT
GTAGAAGGTATTTTCGCCAATATTGTTGTAAATACGGCCGTGCTTTGTACACTTCGAATGAAAGATGATGCTGGTAAAGT
CATTGCGATGATTTTCATTATTTTCATCTTTGCATTCTTAGGATTTGAGCACGTTATCGCCAACTTCTCCTCTTTCTCAT
TAGCATTTTTCGCTTCTGGTGGAACACTTGCTGCAATGACAGCGGGCAATGTGACCGTCAATTTAGTACTTGCTTTACTT
GGGAATTTTGTAGGTGGAGGCCTTGTTATCGGACTCGGCTATGCTTGGCTTAACCGAACGAAATCAATTTATAAAGACTA
A

Upstream 100 bases:

>100_bases
TTTTTATTTTCTGAAAACTATTTGCATCAAATTAAAAACATGCTACAATAAACAAGTTGTGAATTTCACAAGAAAATTTT
TTGTTTGGAGATGAAATAAT

Downstream 100 bases:

>100_bases
TTAAAGGAGACCCCGGAAATTTTCCGAGGTCTCTTTTTTATATGCTTTTAACAGGTAAATTACTTAAAAATGACCGAATG
AGCGGTTCGAATTTCGGGAC

Product: transport protein

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MDVESSPLMQKIDYSTRKKIDLVNNSILRYIVRAMLACLFLTLGTAVAVMIGDKVDHFAPGLGKITYAFMFSWSLVMIIY
MNAELGTSNMMYMTTGVYQKIVKPGKALQILLLCIVCNLLGGILAGYLVSLTSVFHNLPADHFLFTAVSGKLEKAPLQIF
VEGIFANIVVNTAVLCTLRMKDDAGKVIAMIFIIFIFAFLGFEHVIANFSSFSLAFFASGGTLAAMTAGNVTVNLVLALL
GNFVGGGLVIGLGYAWLNRTKSIYKD

Sequences:

>Translated_266_residues
MDVESSPLMQKIDYSTRKKIDLVNNSILRYIVRAMLACLFLTLGTAVAVMIGDKVDHFAPGLGKITYAFMFSWSLVMIIY
MNAELGTSNMMYMTTGVYQKIVKPGKALQILLLCIVCNLLGGILAGYLVSLTSVFHNLPADHFLFTAVSGKLEKAPLQIF
VEGIFANIVVNTAVLCTLRMKDDAGKVIAMIFIIFIFAFLGFEHVIANFSSFSLAFFASGGTLAAMTAGNVTVNLVLALL
GNFVGGGLVIGLGYAWLNRTKSIYKD
>Mature_266_residues
MDVESSPLMQKIDYSTRKKIDLVNNSILRYIVRAMLACLFLTLGTAVAVMIGDKVDHFAPGLGKITYAFMFSWSLVMIIY
MNAELGTSNMMYMTTGVYQKIVKPGKALQILLLCIVCNLLGGILAGYLVSLTSVFHNLPADHFLFTAVSGKLEKAPLQIF
VEGIFANIVVNTAVLCTLRMKDDAGKVIAMIFIIFIFAFLGFEHVIANFSSFSLAFFASGGTLAAMTAGNVTVNLVLALL
GNFVGGGLVIGLGYAWLNRTKSIYKD

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=255, Percent_Identity=27.843137254902, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 28915; Mature: 28915

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVESSPLMQKIDYSTRKKIDLVNNSILRYIVRAMLACLFLTLGTAVAVMIGDKVDHFAP
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
GLGKITYAFMFSWSLVMIIYMNAELGTSNMMYMTTGVYQKIVKPGKALQILLLCIVCNLL
CHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GGILAGYLVSLTSVFHNLPADHFLFTAVSGKLEKAPLQIFVEGIFANIVVNTAVLCTLRM
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEE
KDDAGKVIAMIFIIFIFAFLGFEHVIANFSSFSLAFFASGGTLAAMTAGNVTVNLVLALL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEECCCHHHHHHHHHH
GNFVGGGLVIGLGYAWLNRTKSIYKD
HHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVESSPLMQKIDYSTRKKIDLVNNSILRYIVRAMLACLFLTLGTAVAVMIGDKVDHFAP
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
GLGKITYAFMFSWSLVMIIYMNAELGTSNMMYMTTGVYQKIVKPGKALQILLLCIVCNLL
CHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GGILAGYLVSLTSVFHNLPADHFLFTAVSGKLEKAPLQIFVEGIFANIVVNTAVLCTLRM
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEE
KDDAGKVIAMIFIIFIFAFLGFEHVIANFSSFSLAFFASGGTLAAMTAGNVTVNLVLALL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEECCCHHHHHHHHHH
GNFVGGGLVIGLGYAWLNRTKSIYKD
HHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]