Click here to switch to the map view.

The map label for this gene is fcbA1 [H]

Identifier: 226222981

GI number: 226222981

Start: 382184

End: 383710

Strand: Reverse

Name: fcbA1 [H]

Synonym: Lm4b_00373

Alternate gene names: 226222981

Gene position: 383710-382184 (Counterclockwise)

Preceding gene: 226222988

Following gene: 226222977

Centisome position: 13.17

GC content: 39.16

Gene sequence:

>1527_bases
ATGACGATAAAAAAGTACGAACCACTTAACCTCTATACCAATTTCAAAAAATCTGCTGAACGCTATCCAGAAATGCCCAT
CCATTTTGATGAAGAGCTTGTTACTTTTCCTGAATTAGGCCTTCACACTACTTACAAAAAATGCGAAGAGGCTATTATCC
AAAAAGCAGCTCATCTACATAAATTCGGTGTACGCAAAGAAGAAAAAGTAATCGTATATAAATCTGCCAAATTCGATTCT
TATATTTTAGCAGTTGCGATTTCTTATATTGGTGCCGTGCCAATCATGGTTTCCCCGCATCTTCCAGCGTCTACTATTGA
TATTTTTGTTAATCGCCTTGACCAACCTTGGTTACTTTTTGATTCTGAGACTTCTGAGAAAAGCCATCAATTAAACAATT
TGCCAGACAGTAGATTGATCAATGCTGAACAATTGTTTAAAGCGCCACTAGATGGCTACACATGCGAACAAGAAGAATTA
CCAAAAGATATGATTGCTTATATGACTCACACTTCTGGAACAACTGGTGTGCCAAAATTAATCGCTCACTCTGCGAATTC
GATGGGTTGGAGAACGAAATACCAACGACGTATTCTTAATTTCATCAAACCAAGAGGGCTTGTAGCTTTCCATATCTCAC
CAGTACATTCTCGTTTTAATATTGGTGTTTCATCCTTGATGTCGCTCGGTTTCCCGCTTCTGCCAATTGCCAATCCGACT
AAAGCGAATGTCGAAAAAGTGCTACGCGAATACAAACCGTATGTGCTCGAAACGCATCCAAATCATTTTGTTCAATGGGC
GTCACTTGCTCGTGAAAAACCAGACGTTTTCCAAAGTATCAAATTTTATCATTCTACTTTTGATGCGATTAATAAAGAAA
CAATGGCGGTTTTCCTTCGTACTTCCGAATATAAAAAACCAATTTTCTTACAAATTTATGGACAAAGTGAATGTGGTCCG
ATGATTTTACGTGGTCATACGCTTCAATCTATTGAAACGCTAAATGCGCGTGATATGGGGATTGGCGTTCCTGGTTTAAC
AGAAGTTCGGATTGTTGACCAAGATGGTAATCCTGTTCCAGCTGGGGTTAGTGGTAATATTCAAATGCTTTCCAAAGGTC
GCGCGCTTACTTATTACAAAGAAGAAGCTCGTTTTGAAGAGAATGTGTACGGACCTTGGTGGGATAGCGGCGATTATGGT
ATGAAAGATGAACAAGGCCGGTTATTCTTGCAAGATCGTCAAGTCGATTTAGTAGAAACAATTGATAGTACACTCGCAAT
TGAAGATAAACTGCTTGATACGCTGACTTTCTTAGATGAAGTTGTCATTATCCGTGGTGAAAATGGCAGCCCGCAACCAA
TTATCGCAGTTCATGATGATAAAGAAATGAATTGGGATGCATGGTGGGACGCAGTTTCTGACCTACCACATATGAATGAG
CCAATTGTCATGAAATATGATGAAATCCCTCGTACTGCAACAATGAAAGTACAACGTTTGCAGATGGAACGAGAATTGAA
AAAATAA

Upstream 100 bases:

>100_bases
AGATTTTTTTACAATGATGTAAATGTTTTCTATTACTACTCCTCATTATTTCCACAAAGTGATATAATAGCAACATCAAA
TATTAAAGGAGCATCGCCAT

Downstream 100 bases:

>100_bases
GTTTCAAAAAAGCAGCTGCGTTATGCAGCTGCTTTTTCCTTTATTTTCTTCTAAGTGTAAGTAATGCTAAACTCGTAAGA
AATGTTCCAATAAAGATTGG

Product: fatty-acid--CoA ligase

Products: NA

Alternate protein names: 4-CBA:CoA ligase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTIKKYEPLNLYTNFKKSAERYPEMPIHFDEELVTFPELGLHTTYKKCEEAIIQKAAHLHKFGVRKEEKVIVYKSAKFDS
YILAVAISYIGAVPIMVSPHLPASTIDIFVNRLDQPWLLFDSETSEKSHQLNNLPDSRLINAEQLFKAPLDGYTCEQEEL
PKDMIAYMTHTSGTTGVPKLIAHSANSMGWRTKYQRRILNFIKPRGLVAFHISPVHSRFNIGVSSLMSLGFPLLPIANPT
KANVEKVLREYKPYVLETHPNHFVQWASLAREKPDVFQSIKFYHSTFDAINKETMAVFLRTSEYKKPIFLQIYGQSECGP
MILRGHTLQSIETLNARDMGIGVPGLTEVRIVDQDGNPVPAGVSGNIQMLSKGRALTYYKEEARFEENVYGPWWDSGDYG
MKDEQGRLFLQDRQVDLVETIDSTLAIEDKLLDTLTFLDEVVIIRGENGSPQPIIAVHDDKEMNWDAWWDAVSDLPHMNE
PIVMKYDEIPRTATMKVQRLQMERELKK

Sequences:

>Translated_508_residues
MTIKKYEPLNLYTNFKKSAERYPEMPIHFDEELVTFPELGLHTTYKKCEEAIIQKAAHLHKFGVRKEEKVIVYKSAKFDS
YILAVAISYIGAVPIMVSPHLPASTIDIFVNRLDQPWLLFDSETSEKSHQLNNLPDSRLINAEQLFKAPLDGYTCEQEEL
PKDMIAYMTHTSGTTGVPKLIAHSANSMGWRTKYQRRILNFIKPRGLVAFHISPVHSRFNIGVSSLMSLGFPLLPIANPT
KANVEKVLREYKPYVLETHPNHFVQWASLAREKPDVFQSIKFYHSTFDAINKETMAVFLRTSEYKKPIFLQIYGQSECGP
MILRGHTLQSIETLNARDMGIGVPGLTEVRIVDQDGNPVPAGVSGNIQMLSKGRALTYYKEEARFEENVYGPWWDSGDYG
MKDEQGRLFLQDRQVDLVETIDSTLAIEDKLLDTLTFLDEVVIIRGENGSPQPIIAVHDDKEMNWDAWWDAVSDLPHMNE
PIVMKYDEIPRTATMKVQRLQMERELKK
>Mature_507_residues
TIKKYEPLNLYTNFKKSAERYPEMPIHFDEELVTFPELGLHTTYKKCEEAIIQKAAHLHKFGVRKEEKVIVYKSAKFDSY
ILAVAISYIGAVPIMVSPHLPASTIDIFVNRLDQPWLLFDSETSEKSHQLNNLPDSRLINAEQLFKAPLDGYTCEQEELP
KDMIAYMTHTSGTTGVPKLIAHSANSMGWRTKYQRRILNFIKPRGLVAFHISPVHSRFNIGVSSLMSLGFPLLPIANPTK
ANVEKVLREYKPYVLETHPNHFVQWASLAREKPDVFQSIKFYHSTFDAINKETMAVFLRTSEYKKPIFLQIYGQSECGPM
ILRGHTLQSIETLNARDMGIGVPGLTEVRIVDQDGNPVPAGVSGNIQMLSKGRALTYYKEEARFEENVYGPWWDSGDYGM
KDEQGRLFLQDRQVDLVETIDSTLAIEDKLLDTLTFLDEVVIIRGENGSPQPIIAVHDDKEMNWDAWWDAVSDLPHMNEP
IVMKYDEIPRTATMKVQRLQMERELKK

Specific function: Catalyzes the formation of chlorobenzoyl-CoA via a 2 step reaction. First 4-chlorobenzoate is adenylated by ATP, followed by acyl transfer from the 4-chlorobenzoyl-AMP intermediate to CoA. Benzoate, 4-bromobenzoate, 4- iodobenzoate and 4-fluorobenzoate al

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=556, Percent_Identity=21.9424460431655, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.2.1.33 [H]

Molecular weight: Translated: 58170; Mature: 58039

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKKYEPLNLYTNFKKSAERYPEMPIHFDEELVTFPELGLHTTYKKCEEAIIQKAAHLH
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KFGVRKEEKVIVYKSAKFDSYILAVAISYIGAVPIMVSPHLPASTIDIFVNRLDQPWLLF
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEE
DSETSEKSHQLNNLPDSRLINAEQLFKAPLDGYTCEQEELPKDMIAYMTHTSGTTGVPKL
ECCCCHHHHHHCCCCCHHCCCHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCCCHHHH
IAHSANSMGWRTKYQRRILNFIKPRGLVAFHISPVHSRFNIGVSSLMSLGFPLLPIANPT
HHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCHHHHHHCCCCCCCCCCCC
KANVEKVLREYKPYVLETHPNHFVQWASLAREKPDVFQSIKFYHSTFDAINKETMAVFLR
HHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
TSEYKKPIFLQIYGQSECGPMILRGHTLQSIETLNARDMGIGVPGLTEVRIVDQDGNPVP
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
AGVSGNIQMLSKGRALTYYKEEARFEENVYGPWWDSGDYGMKDEQGRLFLQDRQVDLVET
CCCCCCEEEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHH
IDSTLAIEDKLLDTLTFLDEVVIIRGENGSPQPIIAVHDDKEMNWDAWWDAVSDLPHMNE
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
PIVMKYDEIPRTATMKVQRLQMERELKK
CEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIKKYEPLNLYTNFKKSAERYPEMPIHFDEELVTFPELGLHTTYKKCEEAIIQKAAHLH
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KFGVRKEEKVIVYKSAKFDSYILAVAISYIGAVPIMVSPHLPASTIDIFVNRLDQPWLLF
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEE
DSETSEKSHQLNNLPDSRLINAEQLFKAPLDGYTCEQEELPKDMIAYMTHTSGTTGVPKL
ECCCCHHHHHHCCCCCHHCCCHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCCCHHHH
IAHSANSMGWRTKYQRRILNFIKPRGLVAFHISPVHSRFNIGVSSLMSLGFPLLPIANPT
HHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCHHHHHHCCCCCCCCCCCC
KANVEKVLREYKPYVLETHPNHFVQWASLAREKPDVFQSIKFYHSTFDAINKETMAVFLR
HHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
TSEYKKPIFLQIYGQSECGPMILRGHTLQSIETLNARDMGIGVPGLTEVRIVDQDGNPVP
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
AGVSGNIQMLSKGRALTYYKEEARFEENVYGPWWDSGDYGMKDEQGRLFLQDRQVDLVET
CCCCCCEEEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHH
IDSTLAIEDKLLDTLTFLDEVVIIRGENGSPQPIIAVHDDKEMNWDAWWDAVSDLPHMNE
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
PIVMKYDEIPRTATMKVQRLQMERELKK
CEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA