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The map label for this gene is tpiA2 [H]

Identifier: 226222972

GI number: 226222972

Start: 375146

End: 375910

Strand: Reverse

Name: tpiA2 [H]

Synonym: Lm4b_00364

Alternate gene names: 226222972

Gene position: 375910-375146 (Counterclockwise)

Preceding gene: 226222973

Following gene: 226222971

Centisome position: 12.91

GC content: 41.96

Gene sequence:

>765_bases
ATGCGTAAACCCTTAGTTGGAATTAATATGAAAAACTATATTAACACTCGGGCTCAGACATCTGAATGGTTAGAAGCGAC
TATCCCTCTTCTTAAAAATTTTTCTGATGTGGATACGTTTATCTTTCCTTCTATGGGCACACTCGAAACGACCGCCAACC
TTTTAGCGGGGACATTATTTGGCTTTGGTCCACAAAATATGGCGCCGGAGAAATCCGGCCCGCTAACTGGTGAATTTTCG
GTGGAATCTATCATTGACTTAAACGCGAACTATGTCGAAATTGGTCACGCAGAACGCAAAAACCTTTTTCATGAGAAAAC
TAGTGAGATAGCGAAGAAGATTAAACTTGCATTGGACGAAAAAATCACTCCGGTTGTCTGTGTGGGTGAAGAAGTCCGTG
CGAATGATACAAATGAATTAAAAAATGCGCTAAAAAAACAAATCGAAGCGCTGTTTCAGACAATTAATTTAGCACAGTGG
GAAAATGTTGTTCTGGCTTATGAACCGGAGTGGGCAATTGGAAAGGCGTCTTCAGCTGAGACAAACTACATCGAAAGCGC
GCATCAAGCGCTGCGTGAAATTATTCGCGAACTAGGCGGCGATGAAACGCTCGTAAGAATTATTTACGGTGGCTCGGTCA
GCAAAGAAAACGCCGCAGAAATTGTCCGTCAAAAAAATGTCGATGGTTTATTCGTCGGAAGATTTGGCCATAAACCGCAA
AATTTTGCCGACATTGTTTCTATTGTTAGCAAAACGAAAGGATGA

Upstream 100 bases:

>100_bases
ACTTGGCGAACGAGCAATTGGTCACCTTGATCCTGGCTCTGAATCGTCTTGGATGTTAATGAATGTCATTTTAGAAAATT
TAAAAAAGGCGGTCTGATTT

Downstream 100 bases:

>100_bases
GTTAGTATGAAAATCGCAATTGGTTGTGACGAAATGGGTTACGAACTAAAACAAACCCTCATCACACGTTTAAAGGAGAA
AAATATTGAGTTTACTGATT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2 [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRKPLVGINMKNYINTRAQTSEWLEATIPLLKNFSDVDTFIFPSMGTLETTANLLAGTLFGFGPQNMAPEKSGPLTGEFS
VESIIDLNANYVEIGHAERKNLFHEKTSEIAKKIKLALDEKITPVVCVGEEVRANDTNELKNALKKQIEALFQTINLAQW
ENVVLAYEPEWAIGKASSAETNYIESAHQALREIIRELGGDETLVRIIYGGSVSKENAAEIVRQKNVDGLFVGRFGHKPQ
NFADIVSIVSKTKG

Sequences:

>Translated_254_residues
MRKPLVGINMKNYINTRAQTSEWLEATIPLLKNFSDVDTFIFPSMGTLETTANLLAGTLFGFGPQNMAPEKSGPLTGEFS
VESIIDLNANYVEIGHAERKNLFHEKTSEIAKKIKLALDEKITPVVCVGEEVRANDTNELKNALKKQIEALFQTINLAQW
ENVVLAYEPEWAIGKASSAETNYIESAHQALREIIRELGGDETLVRIIYGGSVSKENAAEIVRQKNVDGLFVGRFGHKPQ
NFADIVSIVSKTKG
>Mature_254_residues
MRKPLVGINMKNYINTRAQTSEWLEATIPLLKNFSDVDTFIFPSMGTLETTANLLAGTLFGFGPQNMAPEKSGPLTGEFS
VESIIDLNANYVEIGHAERKNLFHEKTSEIAKKIKLALDEKITPVVCVGEEVRANDTNELKNALKKQIEALFQTINLAQW
ENVVLAYEPEWAIGKASSAETNYIESAHQALREIIRELGGDETLVRIIYGGSVSKENAAEIVRQKNVDGLFVGRFGHKPQ
NFADIVSIVSKTKG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=31.8548387096774, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=31.9838056680162, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1790353, Length=254, Percent_Identity=33.0708661417323, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=33.4661354581673, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320255, Length=224, Percent_Identity=33.4821428571429, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28572004, Length=197, Percent_Identity=35.5329949238579, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28572008, Length=196, Percent_Identity=35.7142857142857, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28572006, Length=196, Percent_Identity=35.7142857142857, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 28109; Mature: 28109

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPLVGINMKNYINTRAQTSEWLEATIPLLKNFSDVDTFIFPSMGTLETTANLLAGTLF
CCCCEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHH
GFGPQNMAPEKSGPLTGEFSVESIIDLNANYVEIGHAERKNLFHEKTSEIAKKIKLALDE
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
KITPVVCVGEEVRANDTNELKNALKKQIEALFQTINLAQWENVVLAYEPEWAIGKASSAE
CCCCEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCH
TNYIESAHQALREIIRELGGDETLVRIIYGGSVSKENAAEIVRQKNVDGLFVGRFGHKPQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
NFADIVSIVSKTKG
CHHHHHHHHHHCCC
>Mature Secondary Structure
MRKPLVGINMKNYINTRAQTSEWLEATIPLLKNFSDVDTFIFPSMGTLETTANLLAGTLF
CCCCEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHH
GFGPQNMAPEKSGPLTGEFSVESIIDLNANYVEIGHAERKNLFHEKTSEIAKKIKLALDE
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
KITPVVCVGEEVRANDTNELKNALKKQIEALFQTINLAQWENVVLAYEPEWAIGKASSAE
CCCCEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCH
TNYIESAHQALREIIRELGGDETLVRIIYGGSVSKENAAEIVRQKNVDGLFVGRFGHKPQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
NFADIVSIVSKTKG
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]