Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 226222865

GI number: 226222865

Start: 253096

End: 253569

Strand: Direct

Name: ispF

Synonym: Lm4b_00256

Alternate gene names: 226222865

Gene position: 253096-253569 (Clockwise)

Preceding gene: 226222864

Following gene: 226222866

Centisome position: 8.69

GC content: 41.98

Gene sequence:

>474_bases
ATGATTAGAATTGGCCAAGGTTATGATGTACATAAACTTGCTTATGACCGAGAACTGATTGTTGGCGGGATTAAAATTCC
TTATGAGAAAGGGTTACTAGGTCACAGTGATGCGGATGTACTTCTTCATGCTATTACTGATGCTATCATAGGCGCAATTG
GCGCTGGTGATATTGGTCATTTTTTTCCGGACACAGATATGGCTTTTAAAGATGCGGATTCAGCTGAGCTACTTGAGGAA
ATTTGGCAGAAGGTAGAGGCGGATGGTTTTCGTCTTGGAAATCTAGATGCTACTATTATTGCTGAAAAGCCAAAAATGGC
GCCATATGTGGAGCAAATGAAGCTTCGAATTGCGGAGTTGCTACATGCGGATTCAGCCCAAGTTAATGTAAAGGCGACTA
CGACAGAGAAATTAGGGTTTACTGGTCGGAAAGAAGGCATCGCAAGTCTAGCCGTTGTCTTACTTGAAAAATAA

Upstream 100 bases:

>100_bases
GTTGCTATTGTCCAAGGAAGCTATTATAATATTAAGCTGACAACTCCTGAAGATATGCCGCTCGCTAAGGCGATTTTAGG
GGAACTTGGAGGGATAGCAA

Downstream 100 bases:

>100_bases
TTGAAGGAGTGAATAATTGTGAGTGAAACAAAACGAGTACGTGTACGTTATGCACCAAGTCCAACTGGATTTTTGCATAT
TGGGAATGCGCGTACTGCCC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MIRIGQGYDVHKLAYDRELIVGGIKIPYEKGLLGHSDADVLLHAITDAIIGAIGAGDIGHFFPDTDMAFKDADSAELLEE
IWQKVEADGFRLGNLDATIIAEKPKMAPYVEQMKLRIAELLHADSAQVNVKATTTEKLGFTGRKEGIASLAVVLLEK

Sequences:

>Translated_157_residues
MIRIGQGYDVHKLAYDRELIVGGIKIPYEKGLLGHSDADVLLHAITDAIIGAIGAGDIGHFFPDTDMAFKDADSAELLEE
IWQKVEADGFRLGNLDATIIAEKPKMAPYVEQMKLRIAELLHADSAQVNVKATTTEKLGFTGRKEGIASLAVVLLEK
>Mature_157_residues
MIRIGQGYDVHKLAYDRELIVGGIKIPYEKGLLGHSDADVLLHAITDAIIGAIGAGDIGHFFPDTDMAFKDADSAELLEE
IWQKVEADGFRLGNLDATIIAEKPKMAPYVEQMKLRIAELLHADSAQVNVKATTTEKLGFTGRKEGIASLAVVLLEK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=61.5384615384615, Blast_Score=186, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17073; Mature: 17073

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGQGYDVHKLAYDRELIVGGIKIPYEKGLLGHSDADVLLHAITDAIIGAIGAGDIGH
CEEECCCCCHHHHHHCCEEEECCEECCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
FFPDTDMAFKDADSAELLEEIWQKVEADGFRLGNLDATIIAEKPKMAPYVEQMKLRIAEL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
LHADSAQVNVKATTTEKLGFTGRKEGIASLAVVLLEK
HCCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRIGQGYDVHKLAYDRELIVGGIKIPYEKGLLGHSDADVLLHAITDAIIGAIGAGDIGH
CEEECCCCCHHHHHHCCEEEECCEECCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
FFPDTDMAFKDADSAELLEEIWQKVEADGFRLGNLDATIIAEKPKMAPYVEQMKLRIAEL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
LHADSAQVNVKATTTEKLGFTGRKEGIASLAVVLLEK
HCCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]