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The map label for this gene is ispD1

Identifier: 226222864

GI number: 226222864

Start: 252405

End: 253103

Strand: Direct

Name: ispD1

Synonym: Lm4b_00255

Alternate gene names: 226222864

Gene position: 252405-253103 (Clockwise)

Preceding gene: 226222863

Following gene: 226222865

Centisome position: 8.67

GC content: 41.63

Gene sequence:

>699_bases
ATGAATTATGAGTTGGTTTTTTTAGCGGCAGGTCAAGGGAAACGTATGAATGCCCAGAAAAATAAAATGTGGCTAGAGTT
AGTTGGTGAACCAATTTTCATTCATGCCTTGCGTCCTTTTTTAGCAGATAATCGTTGTTCCAAAGTGATTGTTGTGAGTC
AAGAAGAGGAAAGAAAGCATGTAAAAGAATTAATGAGTCAGCTTAACGTTGCTGAAAGCCGAATTGAGATTGTGAAGGGC
GGAAGTGAACGCCAATATAGTGTGGCAGCAGGTCTTGAACGTTGTGGGACGGAGCGGGTTGTCTTAGTACATGATGGCGC
AAGACCGTTTATCACGTTAGATATTATTGATCGATTATTAATTGGTGTAGAGCAAAGTAAAGCCGCGATTTGTGCAGTAA
AAGTAAAAGATACGGTGAAACGAGTGGTGAAAGGTGTCGTTCAAGAAACTGTTGACCGTGAAAATCTTTGGCAAGTTCAA
ACGCCGCAAGCTTTTGAACTGCCTATTTTACGAAAAGCGCATCAGCTTGCTCGAAAAGAACAGTTTTTAGGAACGGATGA
AGCTAGTCTGGTTGAGCGACTTCCTTGTCCTGTTGCTATTGTCCAAGGAAGCTATTATAATATTAAGCTGACAACTCCTG
AAGATATGCCGCTCGCTAAGGCGATTTTAGGGGAACTTGGAGGGATAGCAAATGATTAG

Upstream 100 bases:

>100_bases
ATTAATGAAACAATCCAAGTGGAAGTAACGAGTGTACTTCAAACATCAGCAGGAAGAATGATTTTTGCTAAACCATCCTG
ATTAAAAGGAGGAAATAAGC

Downstream 100 bases:

>100_bases
AATTGGCCAAGGTTATGATGTACATAAACTTGCTTATGACCGAGAACTGATTGTTGGCGGGATTAAAATTCCTTATGAGA
AAGGGTTACTAGGTCACAGT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1; MEP cytidylyltransferase 1; MCT 1

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNYELVFLAAGQGKRMNAQKNKMWLELVGEPIFIHALRPFLADNRCSKVIVVSQEEERKHVKELMSQLNVAESRIEIVKG
GSERQYSVAAGLERCGTERVVLVHDGARPFITLDIIDRLLIGVEQSKAAICAVKVKDTVKRVVKGVVQETVDRENLWQVQ
TPQAFELPILRKAHQLARKEQFLGTDEASLVERLPCPVAIVQGSYYNIKLTTPEDMPLAKAILGELGGIAND

Sequences:

>Translated_232_residues
MNYELVFLAAGQGKRMNAQKNKMWLELVGEPIFIHALRPFLADNRCSKVIVVSQEEERKHVKELMSQLNVAESRIEIVKG
GSERQYSVAAGLERCGTERVVLVHDGARPFITLDIIDRLLIGVEQSKAAICAVKVKDTVKRVVKGVVQETVDRENLWQVQ
TPQAFELPILRKAHQLARKEQFLGTDEASLVERLPCPVAIVQGSYYNIKLTTPEDMPLAKAILGELGGIAND
>Mature_232_residues
MNYELVFLAAGQGKRMNAQKNKMWLELVGEPIFIHALRPFLADNRCSKVIVVSQEEERKHVKELMSQLNVAESRIEIVKG
GSERQYSVAAGLERCGTERVVLVHDGARPFITLDIIDRLLIGVEQSKAAICAVKVKDTVKRVVKGVVQETVDRENLWQVQ
TPQAFELPILRKAHQLARKEQFLGTDEASLVERLPCPVAIVQGSYYNIKLTTPEDMPLAKAILGELGGIAND

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=38.1165919282511, Blast_Score=120, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD1_LISMF (Q724H7)

Other databases:

- EMBL:   AE017262
- RefSeq:   YP_012857.1
- ProteinModelPortal:   Q724H7
- SMR:   Q724H7
- STRING:   Q724H7
- GeneID:   2797930
- GenomeReviews:   AE017262_GR
- KEGG:   lmf:LMOf2365_0247
- TIGR:   LMOf2365_0247
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- ProtClustDB:   PRK13385
- BioCyc:   LMON265669:LMOF2365_0247-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25899; Mature: 25899

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYELVFLAAGQGKRMNAQKNKMWLELVGEPIFIHALRPFLADNRCSKVIVVSQEEERKH
CCEEEEEEECCCCCCCCCHHHHEEEEHHCCCCEEEHHHHHHCCCCCCEEEEECCHHHHHH
VKELMSQLNVAESRIEIVKGGSERQYSVAAGLERCGTERVVLVHDGARPFITLDIIDRLL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHH
IGVEQSKAAICAVKVKDTVKRVVKGVVQETVDRENLWQVQTPQAFELPILRKAHQLARKE
HCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
QFLGTDEASLVERLPCPVAIVQGSYYNIKLTTPEDMPLAKAILGELGGIAND
HHCCCCHHHHHHHCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNYELVFLAAGQGKRMNAQKNKMWLELVGEPIFIHALRPFLADNRCSKVIVVSQEEERKH
CCEEEEEEECCCCCCCCCHHHHEEEEHHCCCCEEEHHHHHHCCCCCCEEEEECCHHHHHH
VKELMSQLNVAESRIEIVKGGSERQYSVAAGLERCGTERVVLVHDGARPFITLDIIDRLL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHH
IGVEQSKAAICAVKVKDTVKRVVKGVVQETVDRENLWQVQTPQAFELPILRKAHQLARKE
HCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
QFLGTDEASLVERLPCPVAIVQGSYYNIKLTTPEDMPLAKAILGELGGIAND
HHCCCCHHHHHHHCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA