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The map label for this gene is radA [H]

Identifier: 226222862

GI number: 226222862

Start: 249806

End: 251179

Strand: Direct

Name: radA [H]

Synonym: Lm4b_00253

Alternate gene names: 226222862

Gene position: 249806-251179 (Clockwise)

Preceding gene: 226222861

Following gene: 226222863

Centisome position: 8.58

GC content: 41.92

Gene sequence:

>1374_bases
ATGGCTAAAGCAAAAAGGACAACCAAATTTGTGTGTCAGGCATGTGGATATGAATCGGCAAAATGGATGGGAAAATGTCC
GAATTGCAACGAGTGGAATCAGATGGTAGAAGCTTTGGAGCCGTCAAAAAAATCACGCTCAGCTTTTAATCATACGGGAG
AACCTTCGAAAGCGACTCCAATTACTCAAATAGCAAGTGAAACAGAAAAACGTGTCGAAACCAATATGCCGGAGTTAAAT
AGAGTTCTTGGCGGAGGTGTGGTTCCAGGATCTATGGTACTTGTCGGTGGGGATCCTGGTATCGGGAAGTCGACTTTATT
ACTGCAAGTTTCAGCTCAACTGACGCTCACAAATAAAAAAGTATTGTATATATCAGGAGAAGAATCTATCAAACAAACGA
AGCTGCGGGCGGAACGCTTGCAAGTTTCGGGAGATAATTTATATGTGTATGCAGAAACAAATTTAGAAGCAGTTCAAGAA
ACGATTGATTTTGTGAAGCCTGATTTTGTAGTCATTGACTCCATTCAGACTGTTTATCATCCTGATGTTACAAGTGCAGC
GGGGAGTGTTTCGCAAGTTAGGGAATGTACAGCGACATTGATGCGAATTGCTAAAATGCAAAACATTGCTATCTTTATTG
TTGGACATGTAACGAAGGAAGGTGCTATTGCGGGGCCGCGCCTACTTGAACATATGGTTGATACTGTGCTTTATTTTGAA
GGGGAGCGTCATCATGCTTACCGGATTTTGCGTGCAGTGAAAAACCGTTTTGGTTCCACTAATGAAATGGGTATATTTGA
AATGCGAGATGTTGGACTTGTAGAAGTTGCTAATCCTTCCGAAGTGTTCTTGGAAGAACGTCTTGAAGGCGCTTCAGGTT
CAACGGTTGTAGCTTCTATGGAAGGAACTCGCCCCGTCCTTGTGGAAATACAAGCGCTTGTTTCGCCTACGATGTTTGGT
AATGCTAAGCGAATGGCGACAGGAATAGACTATAATAAAGTTTCGCTAATTATGGCTGTTTTAGAAAAGCGAGTAGGTTT
AATGTTGCAAAACCAAGATGCTTATTTGAAAGCAGCTGGTGGGGTTAAATTGGATGAACCTGCAGTGGATTTGGCGGTTG
CAGTCAGTGTTGCATCTAGCTATCGTGATAAACCAACAAGAAGTACGGATTGCTTTATTGGAGAACTTGGACTTACAGGT
GAGATTCGTCGTGTTGCAAGAATTGAACAACGCGTACAAGAAGCCGCAAAACTTGGCTTTAAGCGAATTTTTATTCCTAA
AAATAACGAAGGTAATTGGAAAATACCGAAAGACGTGCAAGTGGTTGGGGTGGAAACGATTGGAGAAGCTTTGAAGAAAG
CTTTACCAGATTAA

Upstream 100 bases:

>100_bases
AAAATCAGGATTCGTGTCAAAAAAACGAATTTGATGAAAAGGATTAACGCGAACAGATGATCTGTGGTAAAATGGAGCAT
ATGTATGGAGGGATGTAGAA

Downstream 100 bases:

>100_bases
AACTAAATACTTCGTTGATTCCGTGAGTACTATTTGCGGTATAGTCTTTTAATAGTGTAAAATAGTGCTATATGTATATT
TGGTAATAGCCCAATTATAT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD

Sequences:

>Translated_457_residues
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD
>Mature_456_residues
AKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELNR
VLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQET
IDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFEG
ERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFGN
AKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTGE
IRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=457, Percent_Identity=43.7636761487965, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49992; Mature: 49861

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATP
CCCCHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCH
ITQIASETEKRVETNMPELNRVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKK
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEEEECCE
VLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQETIDFVKPDFVVIDSIQTVYH
EEEEECCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEECHHHHCC
PDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
CCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHEEC
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASM
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCCCCEEEEEC
EGTRPVLVEIQALVSPTMFGNAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAG
CCCCCHHEEHHHHHCCHHCCCCHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCEEEECC
GVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTGEIRRVARIEQRVQEAAKLGF
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
KRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD
EEEEEECCCCCCEECCCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATP
CCCHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCH
ITQIASETEKRVETNMPELNRVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKK
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEEEECCE
VLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQETIDFVKPDFVVIDSIQTVYH
EEEEECCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEECHHHHCC
PDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
CCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHEEC
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASM
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCCCCEEEEEC
EGTRPVLVEIQALVSPTMFGNAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAG
CCCCCHHEEHHHHHCCHHCCCCHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCEEEECC
GVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTGEIRRVARIEQRVQEAAKLGF
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
KRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD
EEEEEECCCCCCEECCCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]