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The map label for this gene is ipk

Identifier: 226222819

GI number: 226222819

Start: 190677

End: 191558

Strand: Direct

Name: ipk

Synonym: Lm4b_00187

Alternate gene names: 226222819

Gene position: 190677-191558 (Clockwise)

Preceding gene: 226222818

Following gene: 226222820

Centisome position: 6.55

GC content: 41.27

Gene sequence:

>882_bases
ATGAAAATAAGCATTACAGCACCAGCTAAAATTAATCTTTCACTTGATGCGCTGTACAAACGTGAGGACGGCTATCATGA
AGTGGAAATGGTGATGACAACAATCGACCTTGCTGATAGATTATACTTAGAACGCTTAGACGAAGACAAAATTGTGCTGG
ATGTAAAAGCACACTTTATTCCAGAAGATCGTCGCAATTTAATTTATCAAGCAGCCCTCCTTTTAAAGAAGCGTTTTGAC
GTAAAGATGGGTGTGCGCATTACAATTGATAAGCACATTCCTGTTTCTGCGGGTCTTGCTGGTGGTAGTTCAGATGCAGC
TGCAGCGCTCAAAGGATTAAATGTCATTTGGGAACTTGGTCTTTCAATAGAAGAATTAGCCGAAATTAGTTCGGAAATTG
GTTCTGATATCGCTTTTTGCGTATACGGCGGAACAGCGCTAGCAACTGGGCGCGGGGAAAAGATTTCTGCGTTACCTAAT
ATTCCTGGTTGTTGGATTGTGCTTGCTAAACCGAGCATTAGCGTGTCTACCCCGACTATCTATAAAGAACTTCAAGTAGA
TAACGTAGAACACCCAGACACACAAAAAATGATTGAATCCATTAAAAATGGCGATTTAGACGGGATATTTGCGTCTACAG
GAAATGTATTAGAGTCTGTGACATTAGAAAAAAATCCGCAAGTTAAACGAATAAAAGATCGGATGTTAGCGTTTGGTGCT
GAGGCGGCCCTAATGAGTGGTAGCGGTCCGACAGTGTTTGCGCTTATCAAACAGTACTCCAGAGCAAAACGTGTCTATAA
CGGCTTGCGTGGCTTTTGTGAGGAAGTTTATATGGTTAGACCATGGAGTGAAGGCGAGAACGATACAAATATAAATAATT
AA

Upstream 100 bases:

>100_bases
TTTTGCTATACATAGAAGTTGTTTTGGGCCGCTAGCTACATATTTTTTCTTTCATATGATAGAATAGAAAAAGAGATTTT
GAACGCGGAGGCCAAGAAAA

Downstream 100 bases:

>100_bases
TGAATGAGAGGGGTCAAAGAAATGAAAATTATTTTTAATGCAGATGATTTTGGAATTAGCCCGGGAGCAGTTTATGGTAT
TTTAGAGTCTTATAAGCGAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKISITAPAKINLSLDALYKREDGYHEVEMVMTTIDLADRLYLERLDEDKIVLDVKAHFIPEDRRNLIYQAALLLKKRFD
VKMGVRITIDKHIPVSAGLAGGSSDAAAALKGLNVIWELGLSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPN
IPGCWIVLAKPSISVSTPTIYKELQVDNVEHPDTQKMIESIKNGDLDGIFASTGNVLESVTLEKNPQVKRIKDRMLAFGA
EAALMSGSGPTVFALIKQYSRAKRVYNGLRGFCEEVYMVRPWSEGENDTNINN

Sequences:

>Translated_293_residues
MKISITAPAKINLSLDALYKREDGYHEVEMVMTTIDLADRLYLERLDEDKIVLDVKAHFIPEDRRNLIYQAALLLKKRFD
VKMGVRITIDKHIPVSAGLAGGSSDAAAALKGLNVIWELGLSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPN
IPGCWIVLAKPSISVSTPTIYKELQVDNVEHPDTQKMIESIKNGDLDGIFASTGNVLESVTLEKNPQVKRIKDRMLAFGA
EAALMSGSGPTVFALIKQYSRAKRVYNGLRGFCEEVYMVRPWSEGENDTNINN
>Mature_293_residues
MKISITAPAKINLSLDALYKREDGYHEVEMVMTTIDLADRLYLERLDEDKIVLDVKAHFIPEDRRNLIYQAALLLKKRFD
VKMGVRITIDKHIPVSAGLAGGSSDAAAALKGLNVIWELGLSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPN
IPGCWIVLAKPSISVSTPTIYKELQVDNVEHPDTQKMIESIKNGDLDGIFASTGNVLESVTLEKNPQVKRIKDRMLAFGA
EAALMSGSGPTVFALIKQYSRAKRVYNGLRGFCEEVYMVRPWSEGENDTNINN

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=271, Percent_Identity=31.3653136531365, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_LISMC (C1KYC3)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002756926.1
- ProteinModelPortal:   C1KYC3
- SMR:   C1KYC3
- GeneID:   7703633
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_00187
- OMA:   RIYNGLR
- ProtClustDB:   PRK00128
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32203; Mature: 32203

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISITAPAKINLSLDALYKREDGYHEVEMVMTTIDLADRLYLERLDEDKIVLDVKAHFI
CEEEEECCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
PEDRRNLIYQAALLLKKRFDVKMGVRITIDKHIPVSAGLAGGSSDAAAALKGLNVIWELG
CCHHHHHHHHHHHHHHHHCCEEECEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
LSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPNIPGCWIVLAKPSISVSTPTI
CCHHHHHHHHHHHCCCEEEEEECCEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCHH
YKELQVDNVEHPDTQKMIESIKNGDLDGIFASTGNVLESVTLEKNPQVKRIKDRMLAFGA
HHHEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHEECCCCCCHHHHHHHHHHHCC
EAALMSGSGPTVFALIKQYSRAKRVYNGLRGFCEEVYMVRPWSEGENDTNINN
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKISITAPAKINLSLDALYKREDGYHEVEMVMTTIDLADRLYLERLDEDKIVLDVKAHFI
CEEEEECCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
PEDRRNLIYQAALLLKKRFDVKMGVRITIDKHIPVSAGLAGGSSDAAAALKGLNVIWELG
CCHHHHHHHHHHHHHHHHCCEEECEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
LSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPNIPGCWIVLAKPSISVSTPTI
CCHHHHHHHHHHHCCCEEEEEECCEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCHH
YKELQVDNVEHPDTQKMIESIKNGDLDGIFASTGNVLESVTLEKNPQVKRIKDRMLAFGA
HHHEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHEECCCCCCHHHHHHHHHHHCC
EAALMSGSGPTVFALIKQYSRAKRVYNGLRGFCEEVYMVRPWSEGENDTNINN
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA