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The map label for this gene is ksgA

Identifier: 226222817

GI number: 226222817

Start: 189274

End: 190161

Strand: Direct

Name: ksgA

Synonym: Lm4b_00185

Alternate gene names: 226222817

Gene position: 189274-190161 (Clockwise)

Preceding gene: 226222816

Following gene: 226222818

Centisome position: 6.5

GC content: 39.75

Gene sequence:

>888_bases
ATGAGTAAAGATATTGCAACTCCCGGAAGAACGACAGAAATCCTAAAGAAGTATGGTTTCTTATTTAAAAAAAGTCTGGG
TCAAAACTTTTTAATCGACAGTAACATTTTGACACGGATTACAGATACAGCCGAAATAACAAAAGAAACAAATGTCATCG
AAATCGGACCAGGTATCGGCGCGCTAACAGAACAATTAGCAAAAACGGCGAACGAAGTAGTTGCTTTTGAAATTGATCAA
CGTTTACTGCCAATTCTTGACGATACGCTAAGTGCATACAATAATATCCAAGTGGTCCACGGAGACGTGCTAAAAGCAGA
TGTGGAGGAAGTTATCGCAGAGCAATTCGCGAAGCCAGATCTACCACTGAAAATCGTAGCCAACCTTCCGTATTACGTAA
CTACACCGATTATTTTAAAATTACTCCATGACAATATTCCGGCAGATTCGATGACGTTTATGCTCCAAAAGGAAGTTGCG
GACCGGATAAGCGCAGTTCCAAGCACGAAAAGCTACGGAAGCCTAACAATCGCTATCCAATTTTACATGGAAGCAGAACT
AGCCTTTATCGTGCCAAAAACTGTCTTCATGCCGCAACCGAATGTTGATTCCGCAGTAATTCATCTGAAGCGTCGCAAAG
AACCATTAGCTGAAGTAAACGACGAAGAATTTTTCTTTGAAGTAACAAGAGCTTCGTTTGCTCAGAGAAGAAAAACGCTT
TGGAATAATTTAGCTTCAAAATTCCCGGCTTTAAAACCACGCAAAGACGAGTTGGTAGAAGGTCTAAATGCTATTGGGAT
AGACTTAATCCGTCGTGGAGAAACGTTGGATATTCCGGAATTTGCTAAATTAAGCAACTTTTTAGGTGATTTTTTAAAGG
AAAAATAA

Upstream 100 bases:

>100_bases
TATACAAACGGAAAACAACTGCAAACCAGACTAGAATCATTTGCAATCAGTGAAGACCAGTTAGTAGCCGCGTGCCAAAA
GATTATGCAGGAGGAAGAAA

Downstream 100 bases:

>100_bases
TTTTTTTTAAAAAAAGCGCTTTCTTAGTTGACAGTTAGCCCTTCCAACTGTTAAAATATTTGGTTATTGATTTCGGAGCC
GAACTATGCTATAATGGATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSKDIATPGRTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAEITKETNVIEIGPGIGALTEQLAKTANEVVAFEIDQ
RLLPILDDTLSAYNNIQVVHGDVLKADVEEVIAEQFAKPDLPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVA
DRISAVPSTKSYGSLTIAIQFYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAEVNDEEFFFEVTRASFAQRRKTL
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLGDFLKEK

Sequences:

>Translated_295_residues
MSKDIATPGRTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAEITKETNVIEIGPGIGALTEQLAKTANEVVAFEIDQ
RLLPILDDTLSAYNNIQVVHGDVLKADVEEVIAEQFAKPDLPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVA
DRISAVPSTKSYGSLTIAIQFYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAEVNDEEFFFEVTRASFAQRRKTL
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLGDFLKEK
>Mature_294_residues
SKDIATPGRTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAEITKETNVIEIGPGIGALTEQLAKTANEVVAFEIDQR
LLPILDDTLSAYNNIQVVHGDVLKADVEEVIAEQFAKPDLPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVAD
RISAVPSTKSYGSLTIAIQFYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAEVNDEEFFFEVTRASFAQRRKTLW
NNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLGDFLKEK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=293, Percent_Identity=31.740614334471, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI7657198, Length=227, Percent_Identity=33.0396475770925, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1786236, Length=278, Percent_Identity=32.3741007194245, Blast_Score=148, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=296, Percent_Identity=28.7162162162162, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25146882, Length=225, Percent_Identity=32.8888888888889, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=29.5964125560538, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21358017, Length=235, Percent_Identity=33.1914893617021, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357273, Length=252, Percent_Identity=26.984126984127, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 33097; Mature: 32965

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIATPGRTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAEITKETNVIEIGPGIG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCHHHHHCCCCEEEECCCCH
ALTEQLAKTANEVVAFEIDQRLLPILDDTLSAYNNIQVVHGDVLKADVEEVIAEQFAKPD
HHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCC
LPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVADRISAVPSTKSYGSLTIAIQ
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
FYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAEVNDEEFFFEVTRASFAQRRKTL
EEEHHHEEEEECCEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHH
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLGDFLKEK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKDIATPGRTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAEITKETNVIEIGPGIG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCHHHHHCCCCEEEECCCCH
ALTEQLAKTANEVVAFEIDQRLLPILDDTLSAYNNIQVVHGDVLKADVEEVIAEQFAKPD
HHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCC
LPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVADRISAVPSTKSYGSLTIAIQ
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
FYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAEVNDEEFFFEVTRASFAQRRKTL
EEEHHHEEEEECCEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHH
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLGDFLKEK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]