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The map label for this gene is rhaS [H]

Identifier: 226222748

GI number: 226222748

Start: 119696

End: 120529

Strand: Direct

Name: rhaS [H]

Synonym: Lm4b_00113

Alternate gene names: 226222748

Gene position: 119696-120529 (Clockwise)

Preceding gene: 226222745

Following gene: 226222749

Centisome position: 4.11

GC content: 37.41

Gene sequence:

>834_bases
ATGCTGAAAATAAATACGACCACATTTAATCCGCAAATTTTGTATATCGCGAATTGTTATACGAATGAGGTGCGCGTAGG
TGAAAATCACCATCATGATTTTTTGGAGATCTCGATTATTTGTGAGGGAAACGTTATTTATGATATTGAAGGCGAGCGAG
TCAAGCTAGGAAAAGGCGATATGCTGATTTTTAATCCTGGTGTGAATCATTACGATATAACCGAACCAGGTATGACGAAT
GCGCAACTCCATATAGGTTTTCGGAACTTTGCACTGGAAGGATACACGCGAAATACGTTCCCGTTTAAAAAGGCCTTTTT
ACGAAAAAAAGAAGAAGAATCTGCCATTTTATCTATTTCTAAACAAATTATAGAAGAAAAAGACGCAGAAAAACCAGGAT
ATGACTTGATTTTAAAGGCTTTCGTCATGCAATTAATTATACATGTTTTACGCGAAGCCACCCCAGAACAACTTGAAAAT
AACGGCGTCAAATTATCTACCGACGAGCAGCAAAAACAGATACTTGTAAACGAAATTATTCATTATATGGAAAAACACCA
CACAGAAGACGTCTCGCTCTCAACGCTTTCGCAAACGATGTACATTAGCCCCGCCTATATCTCCAAAGTATTTAAAGAAG
AAACCGGGGAATCACCAATTAACTACTTAATCAAAATTCGTCTTACTCGTGCAGAAGAATTACTCAAGAATAAAGACGTT
ACCGTAAAACAGGCCGCAAATATGGTCGGATATAATGATGCTTATTATTTTAGCAAGCTTTTCAAGAAATATTACGGGTT
TCCACCATCAGAAAACTGGCGCAAATCGAGTTAG

Upstream 100 bases:

>100_bases
CTCCTAATTTCGAAAAAGAATGGTTTATATTCGCTGTAAGCATGGACAATGTTATATAATTAAAAGATAAATGTAAGCGT
TATAACGAGGAGGTGAAGTG

Downstream 100 bases:

>100_bases
AATTCCAAAATAAGACAGAGAGAAGGAAGACTGGATGGAATATAAAAAAGCTTTAGCGTTTATAAAATCAAATAGCATTC
TAGAACAAGAAGAAGGAACA

Product: transcriptional regulatory protein, AraC family

Products: NA

Alternate protein names: L-rhamnose operon regulatory protein rhaS [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLKINTTTFNPQILYIANCYTNEVRVGENHHHDFLEISIICEGNVIYDIEGERVKLGKGDMLIFNPGVNHYDITEPGMTN
AQLHIGFRNFALEGYTRNTFPFKKAFLRKKEEESAILSISKQIIEEKDAEKPGYDLILKAFVMQLIIHVLREATPEQLEN
NGVKLSTDEQQKQILVNEIIHYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDV
TVKQAANMVGYNDAYYFSKLFKKYYGFPPSENWRKSS

Sequences:

>Translated_277_residues
MLKINTTTFNPQILYIANCYTNEVRVGENHHHDFLEISIICEGNVIYDIEGERVKLGKGDMLIFNPGVNHYDITEPGMTN
AQLHIGFRNFALEGYTRNTFPFKKAFLRKKEEESAILSISKQIIEEKDAEKPGYDLILKAFVMQLIIHVLREATPEQLEN
NGVKLSTDEQQKQILVNEIIHYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDV
TVKQAANMVGYNDAYYFSKLFKKYYGFPPSENWRKSS
>Mature_277_residues
MLKINTTTFNPQILYIANCYTNEVRVGENHHHDFLEISIICEGNVIYDIEGERVKLGKGDMLIFNPGVNHYDITEPGMTN
AQLHIGFRNFALEGYTRNTFPFKKAFLRKKEEESAILSISKQIIEEKDAEKPGYDLILKAFVMQLIIHVLREATPEQLEN
NGVKLSTDEQQKQILVNEIIHYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDV
TVKQAANMVGYNDAYYFSKLFKKYYGFPPSENWRKSS

Specific function: Activates expression of the rhaBAD and rhaT operons [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HTH araC/xylS-type DNA-binding domains [H]

Homologues:

Organism=Escherichia coli, GI1790339, Length=249, Percent_Identity=22.4899598393574, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787987, Length=105, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790391, Length=106, Percent_Identity=35.8490566037736, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788030, Length=254, Percent_Identity=22.8346456692913, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32043; Mature: 32043

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKINTTTFNPQILYIANCYTNEVRVGENHHHDFLEISIICEGNVIYDIEGERVKLGKGD
CEEEEEEECCCEEEEEEECCCCCEECCCCCCCCEEEEEEEECCCEEEEECCCEEEECCCC
MLIFNPGVNHYDITEPGMTNAQLHIGFRNFALEGYTRNTFPFKKAFLRKKEEESAILSIS
EEEECCCCCEEECCCCCCCCCEEEEEEEHEEEECCCCCCCHHHHHHHHCCHHHHHHHHHH
KQIIEEKDAEKPGYDLILKAFVMQLIIHVLREATPEQLENNGVKLSTDEQQKQILVNEII
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHH
HYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDV
HHHHHHCCCCCCHHHHHHHEEECHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHCCCC
TVKQAANMVGYNDAYYFSKLFKKYYGFPPSENWRKSS
CHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLKINTTTFNPQILYIANCYTNEVRVGENHHHDFLEISIICEGNVIYDIEGERVKLGKGD
CEEEEEEECCCEEEEEEECCCCCEECCCCCCCCEEEEEEEECCCEEEEECCCEEEECCCC
MLIFNPGVNHYDITEPGMTNAQLHIGFRNFALEGYTRNTFPFKKAFLRKKEEESAILSIS
EEEECCCCCEEECCCCCCCCCEEEEEEEHEEEECCCCCCCHHHHHHHHCCHHHHHHHHHH
KQIIEEKDAEKPGYDLILKAFVMQLIIHVLREATPEQLENNGVKLSTDEQQKQILVNEII
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHH
HYMEKHHTEDVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDV
HHHHHHCCCCCCHHHHHHHEEECHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHCCCC
TVKQAANMVGYNDAYYFSKLFKKYYGFPPSENWRKSS
CHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA