Click here to switch to the map view.

The map label for this gene is lip2 [H]

Identifier: 226222749

GI number: 226222749

Start: 120564

End: 121679

Strand: Direct

Name: lip2 [H]

Synonym: Lm4b_00114

Alternate gene names: 226222749

Gene position: 120564-121679 (Clockwise)

Preceding gene: 226222748

Following gene: 226222750

Centisome position: 4.14

GC content: 42.29

Gene sequence:

>1116_bases
ATGGAATATAAAAAAGCTTTAGCGTTTATAAAATCAAATAGCATTCTAGAACAAGAAGAAGGAACAGAAGTTATTTTTAA
ACAGGCACAAGAAACAGTTCGAGGGAATTTGGATCCTTTTTTATTAAAAGACCTGAAACAACATCTTGGCGGTACGAATG
ATGCGATAAAAGAAGCGCCGCAAGAAATCCAAATGCCAGATTTTTCTAATTTGGAAATAGCAACGGCAGCTGCACTACAA
ATGCGAGCAACGATGGGAAGTCCGAATATTGATTTATCAAACGGTGTAACTACGGAATATCGGGTTGTTGAAGGGGACTA
TGGCGATATTCCGGTACGAATTTATCGTCACGAAGAAGCGACCAAACCAGTTCCCGCATTCATTTTTTATCATGGTGGTG
GCTTTGTTGGTGGAACGCCGGCTGTTGTGGAGAACTTTTGTAAAGGTATTGCGGAAAAGTTACCGGCAGTTGTTATTAAT
GTGGACTATCATTTAGCTCCCGAATTCCCGGCACCAGCAGCTCCAAAAGATTGTTACAGGGCGCTTGAATGGGTAGTGGA
ACAAAGCGACGAACTCGGTATCGACGCTTCGAAAATAGGTGTTTCTGGAGATAGCGCGGGTGGAACTTTAGCAGCAGCGG
TGAGCTATATGGATTATGAAGCAGAGACGAATTATGTAGGATTTCAAGCTTTACTTTACCCAGCGCTAACGTTAGTAGAT
GAAGATAATGATAAGTACCAGTGGGATATTTCCAAGTTTGGGGCATCAGAAGAGACACTTCCGCTCGTTGCACCAGGTAT
CATTGGAATGAACAGCTCTGGCGAATTACTGCGGACGGCCTATGTTAGAGACGAAAATCCCGCATCGCCAATTTATTCTC
CACTATCGGCCGTAGATAAAAGTATTTATCCGCCTACACTTATAGCCAGTGCGGAGTTTGACGCGTTACGAGCTTTTGCA
GATATTTTTGCTAAAGAACTGCGAGCAAGTGGTGTGCAAACAAAAGCAATCGTTTACCAAGGAATGTGTCATGCTTTCAT
TGATAAATACGGGATTTTCCCTCAAGCGGAAGACGTGGCAGATGAAATTGTTCAAATGATGAAAGAAATATTTTAA

Upstream 100 bases:

>100_bases
TTTAGCAAGCTTTTCAAGAAATATTACGGGTTTCCACCATCAGAAAACTGGCGCAAATCGAGTTAGAATTCCAAAATAAG
ACAGAGAGAAGGAAGACTGG

Downstream 100 bases:

>100_bases
AGAATAATTTGCAGGCATTTTCCGGAATGCCTGTTTGTTTTTGGACTAAATTTTGCGATTAGTACTAAAAATGTATTAGA
ATAGAGAAGAGAAATGAGAA

Product: lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MEYKKALAFIKSNSILEQEEGTEVIFKQAQETVRGNLDPFLLKDLKQHLGGTNDAIKEAPQEIQMPDFSNLEIATAAALQ
MRATMGSPNIDLSNGVTTEYRVVEGDYGDIPVRIYRHEEATKPVPAFIFYHGGGFVGGTPAVVENFCKGIAEKLPAVVIN
VDYHLAPEFPAPAAPKDCYRALEWVVEQSDELGIDASKIGVSGDSAGGTLAAAVSYMDYEAETNYVGFQALLYPALTLVD
EDNDKYQWDISKFGASEETLPLVAPGIIGMNSSGELLRTAYVRDENPASPIYSPLSAVDKSIYPPTLIASAEFDALRAFA
DIFAKELRASGVQTKAIVYQGMCHAFIDKYGIFPQAEDVADEIVQMMKEIF

Sequences:

>Translated_371_residues
MEYKKALAFIKSNSILEQEEGTEVIFKQAQETVRGNLDPFLLKDLKQHLGGTNDAIKEAPQEIQMPDFSNLEIATAAALQ
MRATMGSPNIDLSNGVTTEYRVVEGDYGDIPVRIYRHEEATKPVPAFIFYHGGGFVGGTPAVVENFCKGIAEKLPAVVIN
VDYHLAPEFPAPAAPKDCYRALEWVVEQSDELGIDASKIGVSGDSAGGTLAAAVSYMDYEAETNYVGFQALLYPALTLVD
EDNDKYQWDISKFGASEETLPLVAPGIIGMNSSGELLRTAYVRDENPASPIYSPLSAVDKSIYPPTLIASAEFDALRAFA
DIFAKELRASGVQTKAIVYQGMCHAFIDKYGIFPQAEDVADEIVQMMKEIF
>Mature_371_residues
MEYKKALAFIKSNSILEQEEGTEVIFKQAQETVRGNLDPFLLKDLKQHLGGTNDAIKEAPQEIQMPDFSNLEIATAAALQ
MRATMGSPNIDLSNGVTTEYRVVEGDYGDIPVRIYRHEEATKPVPAFIFYHGGGFVGGTPAVVENFCKGIAEKLPAVVIN
VDYHLAPEFPAPAAPKDCYRALEWVVEQSDELGIDASKIGVSGDSAGGTLAAAVSYMDYEAETNYVGFQALLYPALTLVD
EDNDKYQWDISKFGASEETLPLVAPGIIGMNSSGELLRTAYVRDENPASPIYSPLSAVDKSIYPPTLIASAEFDALRAFA
DIFAKELRASGVQTKAIVYQGMCHAFIDKYGIFPQAEDVADEIVQMMKEIF

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=296, Percent_Identity=27.7027027027027, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI206597554, Length=296, Percent_Identity=28.3783783783784, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI68051721, Length=165, Percent_Identity=29.0909090909091, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21328446, Length=100, Percent_Identity=38, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157041239, Length=331, Percent_Identity=23.8670694864048, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI61966717, Length=140, Percent_Identity=29.2857142857143, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI226423947, Length=173, Percent_Identity=27.7456647398844, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=283, Percent_Identity=26.5017667844523, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=316, Percent_Identity=25.9493670886076, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI72001146, Length=152, Percent_Identity=34.8684210526316, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567059, Length=278, Percent_Identity=24.8201438848921, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=151, Percent_Identity=29.1390728476821, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656084, Length=94, Percent_Identity=37.2340425531915, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656076, Length=94, Percent_Identity=37.2340425531915, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130169, Length=94, Percent_Identity=37.2340425531915, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 40506; Mature: 40506

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKKALAFIKSNSILEQEEGTEVIFKQAQETVRGNLDPFLLKDLKQHLGGTNDAIKEAP
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCH
QEIQMPDFSNLEIATAAALQMRATMGSPNIDLSNGVTTEYRVVEGDYGDIPVRIYRHEEA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCC
TKPVPAFIFYHGGGFVGGTPAVVENFCKGIAEKLPAVVINVDYHLAPEFPAPAAPKDCYR
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECEEECCCCCCCCCCHHHHH
ALEWVVEQSDELGIDASKIGVSGDSAGGTLAAAVSYMDYEAETNYVGFQALLYPALTLVD
HHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEE
EDNDKYQWDISKFGASEETLPLVAPGIIGMNSSGELLRTAYVRDENPASPIYSPLSAVDK
CCCCCEEEEHHHCCCCCCCCCEECCCEEECCCCCCEEHEEEECCCCCCCHHHHHHHHHHH
SIYPPTLIASAEFDALRAFADIFAKELRASGVQTKAIVYQGMCHAFIDKYGIFPQAEDVA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
DEIVQMMKEIF
HHHHHHHHHHC
>Mature Secondary Structure
MEYKKALAFIKSNSILEQEEGTEVIFKQAQETVRGNLDPFLLKDLKQHLGGTNDAIKEAP
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCH
QEIQMPDFSNLEIATAAALQMRATMGSPNIDLSNGVTTEYRVVEGDYGDIPVRIYRHEEA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCC
TKPVPAFIFYHGGGFVGGTPAVVENFCKGIAEKLPAVVINVDYHLAPEFPAPAAPKDCYR
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECEEECCCCCCCCCCHHHHH
ALEWVVEQSDELGIDASKIGVSGDSAGGTLAAAVSYMDYEAETNYVGFQALLYPALTLVD
HHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEE
EDNDKYQWDISKFGASEETLPLVAPGIIGMNSSGELLRTAYVRDENPASPIYSPLSAVDK
CCCCCEEEEHHHCCCCCCCCCEECCCEEECCCCCCEEHEEEECCCCCCCHHHHHHHHHHH
SIYPPTLIASAEFDALRAFADIFAKELRASGVQTKAIVYQGMCHAFIDKYGIFPQAEDVA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
DEIVQMMKEIF
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]