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The map label for this gene is thiN [H]

Identifier: 225861799

GI number: 225861799

Start: 1837381

End: 1838043

Strand: Reverse

Name: thiN [H]

Synonym: SPT_1961

Alternate gene names: 225861799

Gene position: 1838043-1837381 (Counterclockwise)

Preceding gene: 225861800

Following gene: 225861798

Centisome position: 87.02

GC content: 45.25

Gene sequence:

>663_bases
ATGAGCGAGTACAAACTCTCAGAAAACAACTGGACTAGGGTTGCAGTTTTTGCAGGCGGAAACCGCGGTCATTATCGGAC
AGATTTTGATGCTTTTGTTGGGGTGGATCGAGGCTCGCTCTGGGTCTTGGAAGAAGACTTACCTCTTGCTCTAGCAGTCG
GAGATTTTGATTCTGTGACTGCAGAAGAGCGACAGGTGATTCAAAAACGTGCCCAGTATTTTGTCCAAGCCCGACCAGAA
AAGGATGATACAGATCTGGAATTGGCTCTCTTAACCATCTTTGAACAAAATCCTCAGGCTCAGGTCACTATTTTCGGTGC
CTTGGGTGGCCGTATTGACCATATGTTGGCCAATGTCTTTCTACCTAGCAATCCTAAGTTGGCACCCTATATGCGTCAAA
TAGAAATTGAGGATGGGCAAAATTTGATTGCCTATTGTTCAGAAGGGACCAGTCAGCTAGAACCTCGTTCAGACTACGAC
TATCTAGCCTTTATGCCAGTTCGGGATAGCCAGCTGACTATTCTTGGAGCTAAGTATGAGTTGACAGAGGAGAATTTTTT
CTTTAAAAAAGTGTACGCTTCTAACGAATATATAGATAGGGAAGTGTCGGTGACTTGCCCAGATGGTTATGTGGTCGTAC
TGCATAGCAAGGACAGGAGGTAG

Upstream 100 bases:

>100_bases
GAAGTGGATGGTGGGATTGATGACCAAACTATTGCTCAAGCCAAAGAAGCTGGTGCGACTGTTTTTGTAGCAGGTTCCTA
TGTCTTTAAGGGAGAAGTCA

Downstream 100 bases:

>100_bases
GATGGAAAGTTTACTTATTCTATTATTAATTGCCAACCTAGCTGGTCTCTTTCTGATTTGGCAAAGGCAGGATAGGCAGG
AGAAACACTTAAGTAAGAGC

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTAEERQVIQKRAQYFVQARPE
KDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR

Sequences:

>Translated_220_residues
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTAEERQVIQKRAQYFVQARPE
KDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
>Mature_219_residues
SEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTAEERQVIQKRAQYFVQARPEK
DDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYDY
LAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 25082; Mature: 24951

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVT
CCCEEECCCCCEEEEEEECCCCCCEECCCHHEEECCCCEEEEEECCCCEEEEECCCCCCC
AEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVF
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHEE
LPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYDYLAFMPVRDSQLTILGAKYE
CCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEE
LTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
ECCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure 
SEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVT
CCEEECCCCCEEEEEEECCCCCCEECCCHHEEECCCCEEEEEECCCCEEEEECCCCCCC
AEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVF
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHEE
LPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYDYLAFMPVRDSQLTILGAKYE
CCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEE
LTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
ECCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]