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The map label for this gene is trpF [H]

Identifier: 225861615

GI number: 225861615

Start: 1650036

End: 1650635

Strand: Reverse

Name: trpF [H]

Synonym: SPT_1736

Alternate gene names: 225861615

Gene position: 1650635-1650036 (Counterclockwise)

Preceding gene: 225861616

Following gene: 225861614

Centisome position: 78.15

GC content: 43.83

Gene sequence:

>600_bases
TTGACAAAGGTTAAAATTTGTGGACTATCGACCAAAGAAGCGGTGGAAACAGCCGTTTCAGCAGGAGCCGACTATATCGG
TTTTGTCTTTGCACCTAGTAAAAGACAGGTGACTTTAGAAGAGGCAGCTGAGTTGGCAAAGCTTATTCCTGCAGATGTGA
AAAAGGTTGGAGTATTTGTTTCACCAAGTCGGGTAGAACTGCTGGAAGCGATTGACAAAGTTGGCTTGGACTTGGTTCAA
GTTCACGGTCAGGTGGCAGCTGATTTATTTGAGAATTTGCCTTGTGCCAGTATTCAGGCTGTGCAGGTAGATGGAAATGG
GCATGTCCCCAATTCTCAGGCAGATTATCTACTCTTTGATGCCCCTGTGGCAGGAAGTGGTCAGTCCTTTGATTGGGGTC
AACTGGATACGACTGGACTAGCACAGCCCTTCTTTATCGCAGGTGGCCTTAATGAAGATAATGTAGTAAAAGCAATTCAA
CATTTTACTCCCTATGCAGTAGATGTATCGAGCGGAGTAGAGACAGATGGACAAAAAGATCATGAAAAGATTAGAAGATT
TATAGAGAGGGTAAAGCATGGCATATCAGGAACCAAATAA

Upstream 100 bases:

>100_bases
CAGGATGCGGAACGACTAGCCCCATACTTTAACGGAATTTTGGTAGGGACAGCTCTTATGCAGGCAGAAAATGTGGCCCA
GAGAATCAAGGAGTTGCAGA

Downstream 100 bases:

>100_bases
AGATGGATTTTACGGAAAATTCGGCGGACGTTTTGTCCCAGAAACATTGATGACAGCAGTTTTGGAGTTGGAGAAGGCCT
ACCGTGAAAGTCAGGCAGAC

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQ
VHGQVAADLFENLPCASIQAVQVDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK

Sequences:

>Translated_199_residues
MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQ
VHGQVAADLFENLPCASIQAVQVDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK
>Mature_198_residues
TKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQV
HGQVAADLFENLPCASIQAVQVDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQH
FTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=185, Percent_Identity=35.1351351351351, Blast_Score=68, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320210, Length=212, Percent_Identity=31.6037735849057, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00697 PRAI [H]

EC number: =5.3.1.24 [H]

Molecular weight: Translated: 21236; Mature: 21105

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFV
CCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCEEE
SPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQVDGNGHVPNSQADYLLFD
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEE
APVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQHFTPYAVDVSSGVETDGQKD
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHH
HEKIRRFIERVKHGISGTK
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFV
CEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCEEE
SPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQVDGNGHVPNSQADYLLFD
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEE
APVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQHFTPYAVDVSSGVETDGQKD
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHH
HEKIRRFIERVKHGISGTK
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA