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The map label for this gene is trpB [H]

Identifier: 225861614

GI number: 225861614

Start: 1648835

End: 1650058

Strand: Reverse

Name: trpB [H]

Synonym: SPT_1735

Alternate gene names: 225861614

Gene position: 1650058-1648835 (Counterclockwise)

Preceding gene: 225861615

Following gene: 225861613

Centisome position: 78.12

GC content: 48.69

Gene sequence:

>1224_bases
ATGGCATATCAGGAACCAAATAAAGATGGATTTTACGGAAAATTCGGCGGACGTTTTGTCCCAGAAACATTGATGACAGC
AGTTTTGGAGTTGGAGAAGGCCTACCGTGAAAGTCAGGCAGACCCAAGTTTCCAAGAGGAATTAAACCAGCTTTTGCGCC
AGTACGTAGGACGTGAAACTCCTCTTTATTACGCAAAAAACTTGACCCAGCATATCGGCGGAGCCAAGATTTATCTCAAA
CGGGAAGACCTTAACCATACAGGAGCCCACAAGATTAACAATGCCTTGGGACAAGTTTGGCTTGCCAAACGCATGGGTAA
AAAGAAAATTATCGCAGAAACTGGTGCTGGTCAGCACGGTGTGGCAACTGCAACTGCTGCGGCCCTCTTTAACATGGAAT
GTACCATCTACATGGGTGAGGAAGATGTCAAACGCCAAGCCCTCAATGTCTTCCGTATGGAGCTTTTGGGAGCTAAGGTC
GAGGCCGTGACAGATGGTTCGCGCGTGCTCAAGGATGCGGTCAATGCAGCCCTTCGTTCATGGGTGGCTAATATAGATGA
TACCCACTATATCCTTGGTTCTGCCTTGGGGCCTCATCCATTTCCAGAAATTGTTCGTGACTTCCAAAGTGTCATTGGTC
GAGAGGCTAAACAACAGTACCGTGACTTGACAGGACAAAATCTGCCAGATGCCCTAGTAGCCTGTGTTGGTGGTGGGTCG
AATGCCATCGGTCTCTTCCATCCCTTTGTAGAAGATGAGTCAGTAGCCATGTATGGAGCTGAAGCGGCTGGATTTGGTGT
GGATACGGAGCACCACGCAGCTACCTTGACCAAGGGTCGTCCAGGTGTCCTTCACGGTTCCCTCATGGATGTGCTCCAAG
ATGCCCATGGTCAAATTCTTGAAGCCTTCTCTATCTCAGCAGGTTTGGACTATCCTGGTATCGGTCCAGAGCATTCTCAC
TACCACGATATTAAACGTGCCAGCTATGTCCCTGTGACAGACGAAGAAGCCTTGGAAGGATTCCAACTCTTGTCTCGTGT
GGAAGGGATTATCCCAGCTTTGGAATCTAGCCATGCTATCGCCTTTGCGGTGAAATTGGCCAAAGAACTTGGACCAGAAA
AGTCTATGATTGTATGCCTATCAGGTCGTGGGGACAAGGATGTGGTTCAAGTAAAAGACCGCTTGGAAGCAGATGCAGCA
AAGAAGGGAGAAGCTCATGCCTAA

Upstream 100 bases:

>100_bases
CAACATTTTACTCCCTATGCAGTAGATGTATCGAGCGGAGTAGAGACAGATGGACAAAAAGATCATGAAAAGATTAGAAG
ATTTATAGAGAGGGTAAAGC

Downstream 100 bases:

>100_bases
GACACTAACAAAAAAATTAAATGCTATTAAAGCAGCTGGAAAAGGAATTTTTGTTCCCTATATCATGGCTGGTGACCACG
AAAAAGGTTTGGATGGTCTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLK
REDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
NAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSH
YHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAA
KKGEAHA

Sequences:

>Translated_407_residues
MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLK
REDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
NAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSH
YHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAA
KKGEAHA
>Mature_406_residues
AYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKR
EDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKVE
AVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGSN
AIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHY
HDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAK
KGEAHA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=389, Percent_Identity=53.2133676092545, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321412, Length=392, Percent_Identity=57.6530612244898, Blast_Score=477, Evalue=1e-135,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 44251; Mature: 44119

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRET
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHG
CHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCC
VATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKVEAVTDGSRVLKDAVNAALRS
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHH
WVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
HHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
NAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQIL
CCEEHCCCCCCCCCEEEECCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHH
EAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAI
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAKKGEAHA
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHG
CHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCC
VATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKVEAVTDGSRVLKDAVNAALRS
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHH
WVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
HHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
NAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQIL
CCEEHCCCCCCCCCEEEECCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHH
EAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAI
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAKKGEAHA
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA