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The map label for this gene is priA [H]

Identifier: 225861558

GI number: 225861558

Start: 1598700

End: 1601096

Strand: Reverse

Name: priA [H]

Synonym: SPT_1674

Alternate gene names: 225861558

Gene position: 1601096-1598700 (Counterclockwise)

Preceding gene: 225861559

Following gene: 225861557

Centisome position: 75.8

GC content: 46.56

Gene sequence:

>2397_bases
ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGTTACAGGATTCCGGAGGAATT
TGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCTTTTGGTAAGGGTAATCGCCTGATTCAAGGGATTGTTCTTG
GTTTGGAGTCCCAATCAGATGGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAAATTTCTATCCTCAAGGCCAT
GCTTCCAGGATTTCTGAATTCTAGCTATGACAAGATTCTCTATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTGCGCC
TGTTTGGTTCAGAAGATTCGCTAGCCTTTTCTTCACTAGACCTTGCCAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
GGCCTGCTTGTTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGGTATGAGGTTGACCATGCTCA
ATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAGTTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCG
CTTCCTTGGCTAGCTTGTTAGAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
CAAAAGGAAGTTCAACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTGGAGTTAAATCCAGAACAAAG
ACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCTCAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGA
AGACCGAGGTTTACTTGCAGATTATCCAAGGTGCCCTGGATAAGGGCAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTTCACTCAGGATTATCCAATGG
TGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGATGCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTC
CTCTGAAAAATCTGGGTGTCATGATTATTGATGAAGAGCATGAAGCGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
GCCAGAGAGGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCTGCAACGCCGAGCTTAGAGAG
CCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGTCTAACCCAACGTGCCAATCCTTTGGCTACAATCCCTGAGG
TTCAAGTGATTGACTTTCGAGACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGCTTTGTCATGTGTCGGGAGTG
TGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATT
GTGGTTTCTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTTTGAGGATGGATGTGGATACGACTCGAAAGAAAGG
CAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTT
TGGATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTA
CAATTCGCAGCACTATGCTATTCGATTTGCCAAGGATCAAGATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGAC
GACAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGC
CCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAATACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGG
TCTTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA

Upstream 100 bases:

>100_bases
TTGCTAAAGAAAAAGAAGATGGTGAAAAAATTTAAGGTTGGGGGGACTCAATCTTATTTTTTCTATTGCAAGAATGTACT
GACAAGGAGGAGGTGAGAAG

Downstream 100 bases:

>100_bases
GAAGGAGAAGATATGACAAAACTAATCTTTATGGGGACCCCCGACTTTTCAGCAACAGTCTTAAAAGGACTTTTGACAGA
TGACCGTTACGAAATTCTAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 798; Mature: 797

Protein sequence:

>798_residues
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
GLLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYH
AREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Sequences:

>Translated_798_residues
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
GLLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYH
AREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
>Mature_797_residues
ALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVL
TPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRKG
LLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIVQ
KEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISL
TPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYHA
REVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQD
RLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQK
AYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSER
TFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRA
YEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=801, Percent_Identity=34.8314606741573, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90057; Mature: 89926

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSD
CCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEEHEEECCCCCC
GEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKIL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEE
YPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRKGLLVLEYQAVDQKKVKTQSW
ECCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHCCCHH
YEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
HCCCCHHCCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHEEEEECCCCHHHH
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQ
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
IIQGALDKGKTAILLVPEISLTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGD
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCC
AQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYHAREVAILRAQYNQATLVLGS
CEEEEECHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCHHHHHEEEEEEECCCEEEEEEC
ATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
CCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCCCCCCCCHHHHHHHH
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDI
HHHCCCCEEEEEEECCCCCCEEEEHHCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCH
PQVCPNCKSRSIRYYGTGTQKAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGE
HHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHCCCC
ADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAG
EEEEECHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
QVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
HHHHHHCCCCEEEEEEECCCCCCCEEEEEHHHHHHCCCCCEEEEEEEEECCCHHHHHHHH
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQE
HHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHC
RENSELRLSIDHEPQQFL
CCCCEEEEEECCCHHHCC
>Mature Secondary Structure 
ALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSD
CHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEEHEEECCCCCC
GEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKIL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEE
YPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRKGLLVLEYQAVDQKKVKTQSW
ECCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHCCCHH
YEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
HCCCCHHCCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHEEEEECCCCHHHH
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQ
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
IIQGALDKGKTAILLVPEISLTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGD
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCC
AQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYHAREVAILRAQYNQATLVLGS
CEEEEECHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCHHHHHEEEEEEECCCEEEEEEC
ATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
CCCCHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCCCCCCCCHHHHHHHH
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDI
HHHCCCCEEEEEEECCCCCCEEEEHHCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCH
PQVCPNCKSRSIRYYGTGTQKAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGE
HHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHCCCC
ADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAG
EEEEECHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
QVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
HHHHHHCCCCEEEEEEECCCCCCCEEEEEHHHHHHCCCCCEEEEEEEEECCCHHHHHHHH
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQE
HHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHC
RENSELRLSIDHEPQQFL
CCCCEEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]