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The map label for this gene is fmt [H]

Identifier: 225861557

GI number: 225861557

Start: 1597752

End: 1598687

Strand: Reverse

Name: fmt [H]

Synonym: SPT_1673

Alternate gene names: 225861557

Gene position: 1598687-1597752 (Counterclockwise)

Preceding gene: 225861558

Following gene: 225861556

Centisome position: 75.69

GC content: 46.26

Gene sequence:

>936_bases
ATGACAAAACTAATCTTTATGGGGACCCCCGACTTTTCAGCAACAGTCTTAAAAGGACTTTTGACAGATGACCGTTACGA
AATTCTAGCCGTTGTGACCCAGCCAGACCGTGCTGTTGGTCGTAAAAAAGTTATCCAAGAAACCCCAGTCAAGCAGGCTG
CCAAGGAAGCAGGACTATCTATCTACCAACCTGAAAAATTATCTGGAAGTCCAGAGATGGAAGATCTTATGAAGCTAGGA
GCAGATGGAATTGTGACTGCTGCTTTTGGGCAGTTTCTCCCAAGCAAACTCCTTGATAGCATGGACTTTGCTGTCAACGT
TCATGCCTCCCTCCTTCCTAGACACCGTGGTGGTGCGCCTATCCATTATGCCTTGATTCAAGGGGATGAGGAAGCTGGTG
TGACCATCATGGAAATGGTTAAGGAAATGGATGCAGGAGATATGATTTCTCGTCGCAGCATTCCGATCACAGATGAGGAC
AATGTTGGCACCTTGTTTGAAAAATTGGCGCTAGTTGGTCGTGATTTGCTTTTGGACACTCTGCCTGCCTATATTGCTGG
TGATATCAAACCTGAACCGCAGGATACGAGTCAGGTTACCTTCTCTCCAAATATAAAGCCAGAGGAAGAAAAACTGGACT
GGAACAAAACCAATCGTCAACTCTTTAACCAAATTCGTGGAATGAACCCCTGGCCTGTTGCCCATACTTTCCTTAAGGGC
GACCGCTTTAAGATTTATGAAGCCCTACCAGTAGAAGGTCAGGGAAATCCAGGTGAGATTCTCTCTATCGGCAAGAAAGA
ATTGATTGTCGCAACGGCTGAAGGGGCTCTATCCCTCAAACAAGTGCAGCCAGCTGGTAAGCCTAAGATGGACATTGCTT
CCTTCCTCAACGGAGTTGGACGTACATTGACTGTAGGAGAACGATTTGGTGACTAA

Upstream 100 bases:

>100_bases
CCTCAACCAGGTCTTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAAT
TTTTATAAGAAGGAGAAGAT

Downstream 100 bases:

>100_bases
AGTAGAAACGGCTAGAAGTTTAGCTCTAGCAGTGCTAGAGGATGTTTTTGTGAACCAAGCATACTCAAATATCGCCTTAA
ATAAGCATCTAAAGGGGAGT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLG
ADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTNRQLFNQIRGMNPWPVAHTFLKG
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD

Sequences:

>Translated_311_residues
MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLG
ADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTNRQLFNQIRGMNPWPVAHTFLKG
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD
>Mature_310_residues
TKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGA
DGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDEDN
VGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTNRQLFNQIRGMNPWPVAHTFLKGD
RFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=268, Percent_Identity=26.1194029850746, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=26.8085106382979, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI21614513, Length=303, Percent_Identity=22.4422442244224, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=40.9677419354839, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=30.2491103202847, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=29.7297297297297, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319458, Length=285, Percent_Identity=25.2631578947368, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550868, Length=321, Percent_Identity=27.1028037383178, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571984, Length=237, Percent_Identity=29.1139240506329, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585660, Length=247, Percent_Identity=24.6963562753036, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33988; Mature: 33856

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLS
CCEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCHHHHHHHCCCE
IYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAP
EECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDEDNVGTLFEKLALVGRDLLLDT
EEEEEEECCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTNRQLFNQIRGMNPWPVAHTFLKG
HHHHHCCCCCCCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCC
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVG
CCEEEEEEECCCCCCCCCHHEECCCCEEEEEECCCCEEHHHCCCCCCCCHHHHHHHHHCC
RTLTVGERFGD
CEEECCHHCCC
>Mature Secondary Structure 
TKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLS
CEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCHHHHHHHCCCE
IYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAP
EECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDEDNVGTLFEKLALVGRDLLLDT
EEEEEEECCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTNRQLFNQIRGMNPWPVAHTFLKG
HHHHHCCCCCCCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCC
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVG
CCEEEEEEECCCCCCCCCHHEECCCCEEEEEECCCCEEHHHCCCCCCCCHHHHHHHHHCC
RTLTVGERFGD
CEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA