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The map label for this gene is alsE [H]

Identifier: 225861447

GI number: 225861447

Start: 1478369

End: 1479064

Strand: Reverse

Name: alsE [H]

Synonym: SPT_1556

Alternate gene names: 225861447

Gene position: 1479064-1478369 (Counterclockwise)

Preceding gene: 225861448

Following gene: 225861446

Centisome position: 70.03

GC content: 36.35

Gene sequence:

>696_bases
ATGTCAAGAATTGAATTTTCACCATCTTTGATGACCATGGATTTGGACAAATTCAAAGAGCAGATTACTTTTTTGAATGA
TAAAGTAGCATCTTATCATATCGATATTATGGATGGCCATTTTGTTCCCAATATTACCTTGTCTCCTTGGTTCATTCAAG
AAGTTCAAAAAATTAGTGACACACCTTTATCAGTTCATCTGATGGTCACAGACCCAACCTTTTGGGTAGATCAAGTTCTC
GATTTACAATGTGAGTATATTTGTATTCATGCTGAAGTTCTGAATGGTCTTGCTTTTCGTTTGATTGATAAAATTCATGA
TGCAGGTCTAAAGGCTGGTGTTGTCCTTAATCCTGAAACACCTGTTTCTACAATCTTTCCCTACATTGATTTACTTGACA
AAGTAACTATTATGACTGTAGATCCAGGTTTTGCAGGACAACGCTTTTTGGAGTCTACCTTGTATAAAATCCAAGAACTC
CGTCAGCTTAGAGTTCAGAATGGTTATCACTACATCATTGAGATGGATGGTTCTTCGAGTCGTAAGACTTTCAAACAAAT
TGATGTGGCAGGACCAGATATTTATGTTATAGGTCGTAGTGGATTATTTGGTTTGGATGACGATATTGCCAAAGCCTGGG
ATATCTGTTCTAGAGATTACGAAGAAATGACCGGAAAAACAATGCCAATCAAATAA

Upstream 100 bases:

>100_bases
TACGCGATTTTGAAAAAACCAATAAAACATACAGAACCAGTTATGACTGTTGAAGAAGAGATTGATTTGTCAGATATTGA
AATTTTGTAAGAGGGTAACG

Downstream 100 bases:

>100_bases
TGGTTTGAGAAGAAATTTATTAGTTAGGAGGAATATATGTCACTACAATCAGTTAACGCCATTCGTTTTCTTGGCGTAGA
TGCTATTAACAAATCTAATT

Product: allulose-6-phosphate 3-epimerase

Products: fructose-6-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPNITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVL
DLQCEYICIHAEVLNGLAFRLIDKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSRDYEEMTGKTMPIK

Sequences:

>Translated_231_residues
MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPNITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVL
DLQCEYICIHAEVLNGLAFRLIDKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSRDYEEMTGKTMPIK
>Mature_230_residues
SRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPNITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLD
LQCEYICIHAEVLNGLAFRLIDKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQELR
QLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSRDYEEMTGKTMPIK

Specific function: D-ALLOSE METABOLISM. ESSENTIAL FOR THIS PATHWAY. [C]

COG id: COG0036

COG function: function code G; Pentose-5-phosphate-3-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribulose-phosphate 3-epimerase family [H]

Homologues:

Organism=Homo sapiens, GI40385883, Length=202, Percent_Identity=31.1881188118812, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI219879828, Length=202, Percent_Identity=30.6930693069307, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790523, Length=214, Percent_Identity=49.0654205607477, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1789788, Length=202, Percent_Identity=32.1782178217822, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790754, Length=155, Percent_Identity=34.1935483870968, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17552948, Length=208, Percent_Identity=31.7307692307692, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322341, Length=208, Percent_Identity=30.2884615384615, Blast_Score=101, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24586301, Length=207, Percent_Identity=30.4347826086957, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000056
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00834 Ribul_P_3_epim [H]

EC number: 5.1.3.- [C]

Molecular weight: Translated: 26331; Mature: 26199

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01086 RIBUL_P_3_EPIMER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPNITLSPWFIQEVQKISD
CCCCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCC
TPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLIDKIHDAGLKAGVVLNPET
CCEEEEEEEECCCHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
PVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQELRQLRVQNGYHYIIEMDGSSS
CHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
RKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSRDYEEMTGKTMPIK
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPNITLSPWFIQEVQKISD
CCCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCC
TPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLIDKIHDAGLKAGVVLNPET
CCEEEEEEEECCCHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
PVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQELRQLRVQNGYHYIIEMDGSSS
CHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
RKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSRDYEEMTGKTMPIK
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-allulose-6-phosphate [C]

Specific reaction: D-allulose-6-phosphate = fructose-6-phosphate [C]

General reaction: Isomerases; Racemases and Epimerases; Acting on Carbohydrates and Derivatives [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 9401019 [H]