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The map label for this gene is fruA [H]

Identifier: 225861448

GI number: 225861448

Start: 1479075

End: 1480160

Strand: Reverse

Name: fruA [H]

Synonym: SPT_1557

Alternate gene names: 225861448

Gene position: 1480160-1479075 (Counterclockwise)

Preceding gene: 225861449

Following gene: 225861447

Centisome position: 70.08

GC content: 40.33

Gene sequence:

>1086_bases
ATGTTGAAACACTTAAACTTAAAAGGTCACTTATTGACAGCCATTTCCTATATGATTCCAATTGTTTGTGGTGCAGGATT
CTTAGTTGCCATTGGTTTAGCAATGGGGGGTGGTGTTCCTGACGCTCTTGTAGCAGGAAAATTCACTATCTGGGATGCTT
TAGCAACTATGGGTGGTAAAGCCCTTGGTCTCTTGCCAGTTGTTATTGCTACAGGTTTGTCTTACTCGATTGCTGGTAAA
CCAGGGATTGCACCAGGTTTTGTTGTTGGTCTAATTGCCAATTCTGTTGGTTCAGGGTTTATCGGTGGTATCTTGGGAGG
TTATATAGCTGGTTTCTTGGTTCAAGCGATTATTAAAAAGGTCAAAGTACCAAACTGGATTAAAGGTTTAATGCCAACCT
TGATTATTCCTTTTGTAGCCTCTTTGGTAAGTAGTTTGATTATGATTTATATTATTGGAGCGCCTATCGCAGCCTTTACC
AACTGGTTGACGAGCTTATTACAAAGCTTGGGAAGTGCTTCAAATGGTTTGATGGGGGCAGTTATTGGAGTTCTCAGTGC
TGTTGACTTTGGTGGCCCACTTAATAAAACAGTCTATGCGTTTGTGTTGACTTTACAGGCTGAAGGTGTGAAAGAACCAT
TGACTGCTTTGCAATTGGTGAATACTGCTACACCAGTTGGATTTGGATTGGCCTATTTTATCGCGAAATTACTCAAAAAA
AATATCTATACTCAAGAGGAAATCGAAACATTGAAATCGGCTGTTCCTATGGGGATTGTCAATATTGTTGAAGGTGTAAT
TCCGATTGTTATGAATAACTTGGTTCCAGGTCTCATTGCAACAGGTATCGGTGGTGCTGTTGGTGGTGCTGTTTCTTTGA
CAATGGGTGCTGATTCTGCCGTGCCATTTGGTGGAGTGCTTATGTTACCAACCATGACTCGTCCAGTAGCTGGTATTTGT
GCTTTGTTAGCCAACATTGTAGTCACAGGACTTGTCTACGCGATTTTGAAAAAACCAATAAAACATACAGAACCAGTTAT
GACTGTTGAAGAAGAGATTGATTTGTCAGATATTGAAATTTTGTAA

Upstream 100 bases:

>100_bases
AAGGTTCCAACAGAAGTGGCAGTCAAATCTCCCAATAAACTGATTGCTAAAGCTGTTGAGATTGTTACGAAATAACTGAA
AATATTTAAGGAGAAAATAT

Downstream 100 bases:

>100_bases
GAGGGTAACGATGTCAAGAATTGAATTTTCACCATCTTTGATGACCATGGATTTGGACAAATTCAAAGAGCAGATTACTT
TTTTGAATGATAAAGTAGCA

Product: PTS system, IIC component

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALVAGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGK
PGIAPGFVVGLIANSVGSGFIGGILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQLVNTATPVGFGLAYFIAKLLKK
NIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVPGLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGIC
ALLANIVVTGLVYAILKKPIKHTEPVMTVEEEIDLSDIEIL

Sequences:

>Translated_361_residues
MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALVAGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGK
PGIAPGFVVGLIANSVGSGFIGGILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQLVNTATPVGFGLAYFIAKLLKK
NIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVPGLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGIC
ALLANIVVTGLVYAILKKPIKHTEPVMTVEEEIDLSDIEIL
>Mature_361_residues
MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALVAGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGK
PGIAPGFVVGLIANSVGSGFIGGILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQLVNTATPVGFGLAYFIAKLLKK
NIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVPGLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGIC
ALLANIVVTGLVYAILKKPIKHTEPVMTVEEEIDLSDIEIL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=336, Percent_Identity=38.0952380952381, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1790386, Length=310, Percent_Identity=34.5161290322581, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1786951, Length=309, Percent_Identity=30.42071197411, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87082348, Length=304, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788729, Length=373, Percent_Identity=24.1286863270777, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 36983; Mature: 36983

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALVAGKFTIWDALATMGGK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHCCC
ALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIGGILGGYIAGFLVQAIIKK
HHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFTNWLTSLLQSLGSASNGLMGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHH
VIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQLVNTATPVGFGLAYFIAKLLKK
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVPGLIATGIGGAVGGAVSLTMGADSA
HCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCC
VPFGGVLMLPTMTRPVAGICALLANIVVTGLVYAILKKPIKHTEPVMTVEEEIDLSDIEI
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCC
L
C
>Mature Secondary Structure
MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALVAGKFTIWDALATMGGK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHCCC
ALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIGGILGGYIAGFLVQAIIKK
HHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFTNWLTSLLQSLGSASNGLMGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHH
VIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQLVNTATPVGFGLAYFIAKLLKK
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVPGLIATGIGGAVGGAVSLTMGADSA
HCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCC
VPFGGVLMLPTMTRPVAGICALLANIVVTGLVYAILKKPIKHTEPVMTVEEEIDLSDIEI
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]