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The map label for this gene is folD [H]

Identifier: 225861277

GI number: 225861277

Start: 1312808

End: 1313686

Strand: Reverse

Name: folD [H]

Synonym: SPT_1376

Alternate gene names: 225861277

Gene position: 1313686-1312808 (Counterclockwise)

Preceding gene: 225861278

Following gene: 225861276

Centisome position: 62.2

GC content: 45.51

Gene sequence:

>879_bases
TTGAAAGAAAAGAGAAGTGATATGACACAGATTATTGATGGGAAAGCTTTAGCGGCCAAATTGCAGGGGCAGTTGGCTGA
AAAGACTGCAAAATTAAAGGAAGAAACAGCTCTAGTGCCTGGTTTGGTAGTGATTTTGGTTGGGGATAATCCAGCCAGCC
AAGTCTACGTTCGCAACAAGGAGAGGTCAGCTCTTGCGGCTGGTTCCCGTAGCGAAGTAGTGCGAGTTCCAGAGACCATT
ACTCAAGAGGAATTGTTAGACCTGATTGCTAAATATAATCAGGATCCAGCTTGGCATGGGATTTTGGTCCAGTTGCCATT
ACCAAAACATATCGATGAAGAGGCGGTTTTATTAGCCATTGACCCAGAAAAGGATGTGGATGGTTTCCATCCCCTAAACA
TGGGACGTCTTTGGTCTGGACATCCAGTCATGATTCCCTCGACACCTGCAGGAATTATGGAAATGTTTCATGAATATGGG
ATTGACTTGGAAGGTAAAAATGCGGTCGTAATCGGTCGTTCAAATATCGTTGGAAAACCTATGGCTCAGCTTCTTTTGGC
TAAAAATGCGACAGTAACCTTGGCCCACTCACGAACTCATAATCTTGCCAAGGTGGCTGCTAAAGCAGATATTCTTGTAG
TCGCAATCGGCCGCGCCAAGTTTGTGACTGCTGACTTTGTCAAACCAGGTGCGGTTGTCATTGACGTTGGGATGAATCGA
GATGAAAATGGGAAGCTCTGTGGAGATGTTGATTATGAGGCAGTTGCACCACTTGCTAGCCATATCACTCCAGTCCCTGG
AGGTGTCGGTCCTATGACCATTACCATGCTGATGGAGCAAACTTATCAAGCAGCACTTCGGACATTGGATAGAAAATAA

Upstream 100 bases:

>100_bases
AAAGTTCAACCAGAGCTTTTTCTTATAGTTTGAAACTATAGGATAACCTAGGAAAGAAGTGTTAGAGGGCACTAGCAGTT
TTTGGTATAATGGAAGATAT

Downstream 100 bases:

>100_bases
GAGAAACATTCGATTAATAGAGTGTATTTTCTATAGCTATATCTAAAATGGTAGAAATGAATATTAAATTTTAGATATAA
GATTACAAAAGGAGGTCTGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETI
TQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYG
IDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK

Sequences:

>Translated_292_residues
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETI
TQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYG
IDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
>Mature_292_residues
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETI
TQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYG
IDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=46.5986394557823, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=43.0034129692833, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=45.9183673469388, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI36796743, Length=215, Percent_Identity=26.5116279069767, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786741, Length=281, Percent_Identity=49.8220640569395, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=42.6116838487972, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.2818791946309, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=43.9446366782007, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=47.682119205298, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=47.6666666666667, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=46.5753424657534, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=46.5753424657534, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=46.5753424657534, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=46.5753424657534, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31525; Mature: 31525

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
ERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAI
CCHHHHCCCCCCEEECCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEE
DPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKNAVVIGRSNIVGKP
CCCCCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCH
MAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
HHHHHHHCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEEEECCCCCCEEEEEECCCC
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
CCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
ERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAI
CCHHHHCCCCCCEEECCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEE
DPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKNAVVIGRSNIVGKP
CCCCCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCH
MAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
HHHHHHHCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEEEECCCCCCEEEEEECCCC
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
CCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA