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The map label for this gene is thiI [H]

Identifier: 225861227

GI number: 225861227

Start: 1258917

End: 1260131

Strand: Reverse

Name: thiI [H]

Synonym: SPT_1320

Alternate gene names: 225861227

Gene position: 1260131-1258917 (Counterclockwise)

Preceding gene: 225861228

Following gene: 225861226

Centisome position: 59.66

GC content: 42.72

Gene sequence:

>1215_bases
ATGCAGTATTCAGAAATTATGATTCGCTACGGAGAGTTGTCAACCAAGGGTAAAAACCGTATGCGTTTCATCAATAAACT
TCGTAATAATATTTCGGACGTTTTGTCTATCTATACTCAAGTTAAGGTAACAGCAGATCGCGACCGTGCCCACGCTTACC
TCAATGGAGCTGATTACACAGCAGTTGCAGAATCTCTCAAACAAGTTTTTGGAATTCAAAACTTTTCTCCTGTTTATAAG
GTTGAAAAATCTGTAGAAGTTTTGAAGTCTTCTGTCCAAGAGATTATGCGGGACATCTACAAGGAAGGTATGACCTTTAA
GATTTCTAGCAAGCGTAGCGACCACAACTTTGAACTTGATAGTCGTGAACTCAACCAAACACTTGGAGGGGCTGTATTCG
AAGCCATTCCAAATGTGCAAGTTCAAATGAAAAGTCCTGACATCAATCTTCAGGTGGAGATTCGTGAAGAAGCAGCCTAT
CTTTCTTATGAAACCATTCGTGGGGCTGGTGGTTTGCCAGTTGGAACTTCAGGTAAAGGGATGCTCATGTTGTCAGGAGG
GATTGACTCACCTGTAGCAGGTTATCTTGCTCTTAAGCGTGGGGTGGATATCGAGGCAGTTCACTTTGCTAGTCCACCAT
ATACTAGTCCTGGTGCCCTCAAGAAAGCGCAGGACTTGACTCGTAAATTGACCAAGTTTGGCGGAAATATCCAGTTTATC
GAGGTGCCTTTCACAGAGATTCAAGAGGAAATCAAAGCCAAAGCGCCAGAAGCTTATTTGATGACTCTAACTCGTCGCTT
TATGATGCGGATTACTGACCGTATTCGTGAGGTACGAAATGGTTTGGTTATCATCAATGGGGAAAGTCTAGGTCAAGTAG
CCAGCCAAACCCTTGAAAGTATGAAGGCTATCAATGCTGTTACCAACACTCCCATCATTCGTCCTGTGGTTACCATGGAC
AAGTTGGAAATCATTGACATCGCCCAGGAAATCGATACCTTTGACATTTCAATCCAACCGTTTGAAGACTGTTGTACCAT
TTTTGCACCAGATCGTCCAAAAACAAATCCTAAAATTAAGAATGCGGAGCAGTACGAAGCGCGTATGGATGTTGAAGGCT
TGGTTGAGCGAGCAGTGGCTGGAATCATGATTACTGAAATCACACCTCAAGCCGAAAAAGATGAAGTTGATGACTTGATT
GATAATCTACTCTAA

Upstream 100 bases:

>100_bases
TTAGCCTAGACTTGGAAAATGATATGAGTCAGGTCGAGCAGTTTTTGACCAAGTTAAAATTGATTTACAATCAAACTAGA
AAAGTAAGATAGGAGCATTC

Downstream 100 bases:

>100_bases
TTCAGAAAATCCAAAAGAATAGCGAAAATCAGTAAAAAAAGTTAGTTTTTTTTCTAAAAACAGGTAAAAAACTAACTTTT
TTTTTATTTTCATGATATAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVTADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYK
VEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMD
KLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLI
DNLL

Sequences:

>Translated_404_residues
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVTADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYK
VEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMD
KLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLI
DNLL
>Mature_404_residues
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVTADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYK
VEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMD
KLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLI
DNLL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=374, Percent_Identity=27.0053475935829, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45161; Mature: 45161

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVTADRDRAHAYLNGADYT
CCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHH
AVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
SRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAYLSYETIRGAGGLPVGTSGKG
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIK
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEEEECCHHHHHEEECCCCCCCCCCCC
NAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLIDNLL
CCHHHHHHCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVTADRDRAHAYLNGADYT
CCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHH
AVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
SRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAYLSYETIRGAGGLPVGTSGKG
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIK
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEEEECCHHHHHEEECCCCCCCCCCCC
NAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLIDNLL
CCHHHHHHCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA