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The map label for this gene is iscS [C]

Identifier: 225861228

GI number: 225861228

Start: 1260140

End: 1261282

Strand: Reverse

Name: iscS [C]

Synonym: SPT_1321

Alternate gene names: 225861228

Gene position: 1261282-1260140 (Counterclockwise)

Preceding gene: 225861230

Following gene: 225861227

Centisome position: 59.72

GC content: 43.57

Gene sequence:

>1143_bases
ATGATTTACTTTGATAATTCAGCGACGACCAAGCCTTATCCTGAAGCCCTTGAAACCTATATGCAGGTCGCTTCAAAAAT
TTTAGGAAACCCATCTAGTCTCCATCGTTTGGGAGACCAAGCAACACGAATCTTAGATGCTTCTCGCCAACAGATTGCAG
ATTTAGTCGGTAAGAAAAGCGATGAAATCTTCTTTACCTCTGGTGGAACAGAAGGGGATAACTGGGTTATCAAGGGTGTG
GCCTTTGAAAAAGCTCAGTTTGGCAAGCACATCATTGTTTCAGCCATTGAACATCCAGCAGTCAAAGAGTCAGCCCTCTG
GTTGAAAAGTCAAGGATTTGAAGTGGATTTTGCTCCAGTTGATAAGAAAGGCTTGGTCGATGTTGAGGCGTTAGCAGGTT
TGATACGGCCTGATACAATCCTCGTTTCCATCATGGCTGTGAACAATGAAATCGGCTCTATCCAACCTATTGAGGCTATT
TCAGAATTCTTGGCAGACAAGCCGACTATTTCCTTCCACGTTGATGCGGTTCAGGCGCTTGCCAAAATTCCGACTGAAAA
GTATCTGACAGAACGGGTGGATTGCGCGACCTTCTCGAGTCATAAATTTCATGGTGTCCGTGGTGTTGGCTTTGTCTATA
TCAAATCTGGCAAGAAGATTACACCTCTTCTTACAGGTGGTGGCCAGGAGCGAGATTATCGTTCGACAACTGAAAATGTG
GCAGGGATTGCAGCGACAGCCAAGGCTCTTCGTTTATCTATGGAAAAGCTAGATATCTTTAGGAGCAAGACTGGGCAGAT
GAAGGCAGTGATTCACCAAGCTCTTCTGAACTATCCGGATATTTTTGTCTTTTCAGATGAGGAAGACTTTGCACCTCATA
TTCTGACTTTTGGAATCAAAGGTGTTCGAGGTGAAGTCATCGTTCACGCCTTTGAAGACTATGATATTTTTATCTCAACA
ACCTCAGCTTGTTCATCTAAGGCAGGAAAACCAGCTGGTACCTTGATTGCCATGGGAGTGGACAAAGATAAGGCCAAGTC
AGCTGTGCGTCTTAGCCTAGACTTGGAAAATGATATGAGTCAGGTCGAGCAGTTTTTGACCAAGTTAAAATTGATTTACA
ATCAAACTAGAAAAGTAAGATAG

Upstream 100 bases:

>100_bases
TTCTTTAAAAAAATCTTAAAAAAAGAGAAATTGCCCTTTCTTGTCGCAAGCTCCGTTTCATGATACAATAGAGGGAGTGA
TTTTAGAAAGCGTGAGAAAC

Downstream 100 bases:

>100_bases
GAGCATTCATGCAGTATTCAGAAATTATGATTCGCTACGGAGAGTTGTCAACCAAGGGTAAAAACCGTATGCGTTTCATC
AATAAACTTCGTAATAATAT

Product: cysteine desulfurase

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLVGKKSDEIFFTSGGTEGDNWVIKGV
AFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALLNYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFIST
TSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR

Sequences:

>Translated_380_residues
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLVGKKSDEIFFTSGGTEGDNWVIKGV
AFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALLNYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFIST
TSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR
>Mature_380_residues
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLVGKKSDEIFFTSGGTEGDNWVIKGV
AFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALLNYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFIST
TSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=34.2696629213483, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI156713448, Length=408, Percent_Identity=32.1078431372549, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI48994898, Length=358, Percent_Identity=39.1061452513966, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1787970, Length=364, Percent_Identity=23.9010989010989, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789175, Length=347, Percent_Identity=26.5129682997118, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=394, Percent_Identity=34.010152284264, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=28.3018867924528, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=356, Percent_Identity=36.5168539325843, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI20129463, Length=359, Percent_Identity=33.1476323119777, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 41678; Mature: 41678

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLVGKKS
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPV
CCEEEECCCCCCCCEEEEEEEECHHHCCCHHHHHHHCCCCHHHHHHEEECCCCEEEECCC
DKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAISEFLADKPTISFHVDAVQAL
CCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHH
AKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
HHCCHHHHHHHHHCHHCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHH
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALLNYPDIFVFSDEEDFAPHILTFGIK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCC
GVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMS
CCCCEEEEEEECCCEEEEEECHHHHCCCCCCCCEEEEECCCCHHCCCEEEEEEECHHHHH
QVEQFLTKLKLIYNQTRKVR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLVGKKS
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPV
CCEEEECCCCCCCCEEEEEEEECHHHCCCHHHHHHHCCCCHHHHHHEEECCCCEEEECCC
DKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAISEFLADKPTISFHVDAVQAL
CCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHH
AKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
HHCCHHHHHHHHHCHHCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHH
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALLNYPDIFVFSDEEDFAPHILTFGIK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCC
GVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMS
CCCCEEEEEEECCCEEEEEECHHHHCCCCCCCCEEEEECCCCHHCCCEEEEEEECHHHHH
QVEQFLTKLKLIYNQTRKVR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]