Click here to switch to the map view.

The map label for this gene is tmk [H]

Identifier: 225861172

GI number: 225861172

Start: 1201915

End: 1202553

Strand: Reverse

Name: tmk [H]

Synonym: SPT_1265

Alternate gene names: 225861172

Gene position: 1202553-1201915 (Counterclockwise)

Preceding gene: 225861173

Following gene: 225861171

Centisome position: 56.94

GC content: 45.54

Gene sequence:

>639_bases
ATGTCAAAAGGATTTTTAGTCTCTCTTGAGGGACCAGAGGGAGCAGGCAAGACCAGTGTTTTAGAGGCTCTGCTACCAAT
TTTAGAGGAAAAAGGAGTAGAGGTGTTGACGACCCGTGAACCTGGCGGAGTCTTGATTGGGGAGAAGATTCGGGAAGTGA
TTTTGGATCCAAGTCATACTCAGATGGATGCTAAAACAGAGCTACTTCTCTATATTGCCAGTCGCAGACAGCATTTGGTG
GAAAAAGTTCTTCCAGCCCTTGAAGCTGGCAAGTTGGTCATCATGGATCGTTTTATCGATAGTTCTGTTGCCTATCAGGG
ATTTGGTCGTGGCTTAGATATTGAAGCCATTGACTGGCTCAATCAGTTTGCGACAGATGGCCTCAAACCCGATTTGACAC
TCTATTTTGACATCGAGGTGGAAGAAGGGCTGGCTCGTATTGCTGCTAATAGTGACCGCGAGGTTAATCGTTTGGATTTG
GAAGGGTTGGACTTGCATAAAAAAGTTCGTCAAGGCTACCTTTCTCTTCTGGATAAAGAGGGAAATCGCATTGTCAAGAT
TGATGCTAGTCTCCCTTTGGAGCAAGTTGTGGAAACTACCAAGTCTGTCTTGTTTGACGGAATGGGCTTGGCCAAATGA

Upstream 100 bases:

>100_bases
CTTTGATTTTCATTGAGTATGAAATGAGAAGACAAAAAGATTGTCACAAACCCCTATTTTTTTGATAGAATAGAAGTAGT
AAAAAAGAAATGAGTTAGAC

Downstream 100 bases:

>100_bases
AACAAGATCAACTAAAGGCTTGGCAACCAGCTCAGTTTGACCGTTTTGTCCGTATCTTAGAACAAGACCAGCTCAATCAC
GCCTATCTCTTTTCAGGTTT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLV
EKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKSVLFDGMGLAK

Sequences:

>Translated_212_residues
MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLV
EKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKSVLFDGMGLAK
>Mature_211_residues
SKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVE
KVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDLE
GLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKSVLFDGMGLAK

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=209, Percent_Identity=38.2775119617225, Blast_Score=117, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322517, Length=198, Percent_Identity=30.8080808080808, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 23369; Mature: 23238

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHT
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHCCCCCC
QMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHH
NQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDLEGLDLHKKVRQGYLSLLDKE
HHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GNRIVKIDASLPLEQVVETTKSVLFDGMGLAK
CCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHT
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHCCCCCC
QMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHH
NQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDLEGLDLHKKVRQGYLSLLDKE
HHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GNRIVKIDASLPLEQVVETTKSVLFDGMGLAK
CCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA