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The map label for this gene is trmFO [H]

Identifier: 225861168

GI number: 225861168

Start: 1198269

End: 1199603

Strand: Reverse

Name: trmFO [H]

Synonym: SPT_1261

Alternate gene names: 225861168

Gene position: 1199603-1198269 (Counterclockwise)

Preceding gene: 225861169

Following gene: 225861167

Centisome position: 56.8

GC content: 45.32

Gene sequence:

>1335_bases
GTGTCTCAATCTTATATCAATGTTATCGGTGCTGGTTTGGCAGGTTCTGAAGCAGCTTACCAAATCGCAGAGCGTGGTAT
TCCAGTTAAACTTTATGAAATGCGTGGTGTCAAGTCGACTCCTCAGCATAAAACAGACAATTTTGCTGAGTTGGTTTGTT
CCAATTCTTTGCGTGGGGATGCTTTGACAAATGCAGTTGGTCTTCTCAAGGAAGAAATGCGTCGCTTGGGTTCTGTTATC
TTGGAATCTGCTGAGGCTACACGTGTTCCTGCCGGTGGAGCCCTTGCGGTGGATCGTGATGGTTTCTCCCAAATGGTAAC
CGAAAAAGTGGTCAACCACCCCTTGATTGAAGTGGTGCGTGATGAAATTACAGAATTGCCAACAGATGTTATTACAGTTG
TGGCGACCGGTCCCTTGACTAGCGATGCCCTGGCTGAAAAGATTCATGCCCTTAATGATGGCGATGGCTTCTATTTCTAC
GATGCAGCAGCGCCTATTATCGATGTCAACACTATCGATATGAGTAAGGTCTATCTCAAATCTCGTTATGATAAGGGAGA
AGCAGCCTATCTCAATGCTCCAATGACCAAGCAAGAGTTTATGGATTTCCATGAAGCCTTGGTCAATGCGGAAGAAGCAC
CGCTCAATTCTTTTGAAAAAGAAAAGTACTTTGAAGGATGTATGCCAATCGAAGTTATGGCCAAACGTGGCATTAAAACT
ATGCTTTATGGCCCTATGAAGCCAGTCGGTCTTGAGTATCCGGACGACTACACAGGACCTCGTGATGGAGAATTTAAAAC
ACCTTATGCGGTTGTGCAACTTCGTCAGGATAATGCAGCTGGTAGCCTCTACAATATCGTTGGTTTCCAGACCCACCTCA
AATGGGGAGAACAAAAGCGTGTCTTCCAAATGATTCCGAGTCTTGAAAATGCGGAGTTTGTCCGTTATGGTGTCATGCAT
CGCAATTCTTACATGGATTCACCAAATCTTCTTGAGCAGACTTACCGTTCTAAGAAACAACCAAATCTCTTCTTTGCTGG
TCAGATGACGGGGGTGGAAGGCTATGTTGAGTCGGCGGCTTCAGGCTTAGTTGCGGGAATTAACGCAGCTCGTCTCTTCA
AGGAAGAAAGCGAGGCTATTTTCCCCGATACGACAGCGATTGGAAGCTTAGCTCATTACATTACCCATGCCGACAGCAAA
CATTTCCAACCAATGAATGTCAATTTTGGGATCATCAAGGAGTTGGAAGGCGAGCGTATCCGTGATAAGAAGGCTCGTTA
TGAAAAAATTGCAGAGCGTGCCCTTGCCGACTTAGAGGAATTTTTGACTGTCTAA

Upstream 100 bases:

>100_bases
GACTTTTTCAGCCTGAGCCAAGAAACTCGAAAGCTCGTAAGTCTGATTGGCTTTTTCAATGTGAATCTTAACTTCACACT
CCCAAAGAGGTATTAGTGTC

Downstream 100 bases:

>100_bases
TTTTTTTGAAAGAATTGCTCATGATACTATAAAAATCTTAGAAATTGTGATAAAATAGGTAGGATGAAAGAAGGAGAGTG
AAAATGGCGAATCCCAAGTA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVI
LESAEATRVPAGGALAVDRDGFSQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPSLENAEFVRYGVMH
RNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPDTTAIGSLAHYITHADSK
HFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV

Sequences:

>Translated_444_residues
MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVI
LESAEATRVPAGGALAVDRDGFSQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPSLENAEFVRYGVMH
RNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPDTTAIGSLAHYITHADSK
HFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV
>Mature_443_residues
SQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVIL
ESAEATRVPAGGALAVDRDGFSQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFYD
AAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTM
LYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPSLENAEFVRYGVMHR
NSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPDTTAIGSLAHYITHADSKH
FQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=85, Percent_Identity=43.5294117647059, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49290; Mature: 49158

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGD
CCCCHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCH
ALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGFSQMVTEKVVNHPLIEVVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHH
DEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFYDAAAPIIDVNTIDMSKVYLK
HHHHHCCHHHEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHH
SRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
HCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCHH
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
VFQMIPSLENAEFVRYGVMHRNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
SGLVAGINAARLFKEESEAIFPDTTAIGSLAHYITHADSKHFQPMNVNFGIIKELEGERI
HHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHH
RDKKARYEKIAERALADLEEFLTV
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGD
CCCHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCH
ALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGFSQMVTEKVVNHPLIEVVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHH
DEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFYDAAAPIIDVNTIDMSKVYLK
HHHHHCCHHHEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHH
SRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
HCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCHH
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
VFQMIPSLENAEFVRYGVMHRNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
SGLVAGINAARLFKEESEAIFPDTTAIGSLAHYITHADSKHFQPMNVNFGIIKELEGERI
HHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHH
RDKKARYEKIAERALADLEEFLTV
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA