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The map label for this gene is nrdE [H]

Identifier: 225860978

GI number: 225860978

Start: 983959

End: 986118

Strand: Reverse

Name: nrdE [H]

Synonym: SPT_1044

Alternate gene names: 225860978

Gene position: 986118-983959 (Counterclockwise)

Preceding gene: 225860979

Following gene: 225860977

Centisome position: 46.69

GC content: 41.53

Gene sequence:

>2160_bases
ATGGGATTAAAACATCTTGAAGACGTGACTTACTTCCGTCTTAATAACGAAATTAACCGTCCTGTTAATGGACAAATCAT
GCTTCATAAAGACAAGGAAGCCTTGGATGCTTTCTTTAAAGAAAATGTAGTTCCAAACACTATGGTTTTTGATTCAATCA
AAGACAAAATCAATTACCTCATTGAACACAACTACATCGAAACAGCCTTTATCAAGAAATACCGTCCAGAGTTCTTGGAA
GAATTGGCTCAATTTATCAAAGACCAAAACTTCCAATTCAAGTCATTCATGGCAGCTTATAAATTTTACAATCAATATGC
CTTAAAGACTAATGACGGTGAATACTATCTTGAAAATATGGAAGACCGTGTCTTCTTCAACGCCCTTTATTTCGCTGATG
GGAATGAAGCTGTTGCAATCGATATTGCCAATGAAATCATCCACCAACGCTACCAACCTGCTACTCCTTCCTTCTTGAAT
GCTGGACGTGCTCGTCGTGGGGAGTTGGTATCTTGTTTCCTAATCCAAGTGACGGATGATATGAACTCTATCGGACGTTC
TATCAACTCAGCTCTTCAACTTTCACGTATTGGTGGTGGTGTGGGAATTACCCTCAGCAACCTTCGTGAAGCTGGTGCAC
CTATCAAAGGCTATGAAGGAGCTGCTTCAGGTGTCGTTCCTGTTATGAAGCTTTTTGAAGACAGCTTCTCTTACTCCAAC
CAATTGGGGCAACGTCAAGGTGCCGGTGTTGTCTACCTCAACGTCTTTCACCCAGATATCATCGCTTTCCTTTCAACTAA
GAAAGAAAACGCTGATGAAAAAGTACGTGTCAAGACTCTATCACTTGGTGTTGTAGTACCTGATAAATTCTACGAATTGG
CTCGTAAAAATGAAGAAATGTACCTCTTCAGCCCTTACTCTGTAGAAAAAGAATACGGTGTGCCATTCAATTACATCGAC
ATTACTGAAAAATACGATGAATTGGTCGCAAATCCAAATATTCGCAAGACAAAAATCAAGGCGCGGGATTTGGAAACTGA
AATCTCTAAATTGCAACAAGAGTCTGGCTATCCTTATGTAGTCAACATCGATACAGCTAACCGTGCAAATCCTGTTGATG
GAAAGATTATCATGAGTAACTTGTGTTCTGAGATTCTTCAAGTTCAAGAACCAAGCTTGATCAACGATGCTCAAGAATTC
CTTCAAATGGGAACAGATGTTTCATGTAATCTGGGTTCAACCAACGTGGTTAACATGATGACTTCACCTGATTTTGGTCG
TTCTATCCGTGCTATGGTTCGTGCCCTTACTTTCGTTACAGATAGTTCACATATCGTAGCTGTACCAACGATTGACCATG
GAAATAGCCAAGCCCACACCTTTGGTCTCGGTGCCATGGGACTGCACAGCTACCTTGCCCAACAACTCATTGAATATGGT
TCACCTGAGTCTGTTGAATTTACAAGCATCTACTTTATGCTTATGAACTACTGGACCTTGGTAGAGTCAAACAATATTGC
GCGTGAACGTGGTATTACCTTCCACAACTTTGAAAAATCAGACTATGCTAACGGAAGTTACTTTGACAAGTATGTAACTG
GTGAATTTGTTCCAACATCAGACCGTGTTAAAGAACTCTTCAAAAATGTTTTTATCCCTGGTGTTGCTGATTGGGCTGAA
CTTCGCGACAAGGTTCAAGAAGATGGTCTTTACCACCAAAATCGCCTTGCTGTAGCGCCAAATGGTTCTATCAGCTATAT
CAACGACGTTTCTGCTTCTATCCACCCGATTACGCAACGTATCGAAGAACGCCAAGAGAAGAAAATTGGTAAAATCTACT
ACCCTGCTGCTGGCTTGTCTACAGAAACCATTCCTTACTACACTTCTGCCTACGATATGGATATGCGTAAAGTCATCGAT
GTTTACGCTGCTGCGACTGAACACGTGGATCAAGGACTTTCACTCACCCTCTTCATGCGCAGTGACATTCCAAAAGGCCT
TTACGAATGGAAGAGGGAAAATAAACAAACGACACGTGACTTGTCCATCCTTCGTAACTATGCCTTTAACAAGGGAATCA
AGTCTATCTACTACGTCCGTACCTTTACAGATGACGGTGGAGAAGTCGGCGCCAACCAATGTGAAAGCTGTGTGATTTAG

Upstream 100 bases:

>100_bases
AACTGAAACAATTAGCATAATCTTAGTACATCATCCAGAAGAAACTGCTTTTAGGGCTAACTTAGAAGCCTTTCTTTTGT
AATTAGATAAGGGAAATTTT

Downstream 100 bases:

>100_bases
TTTCTAGCATAGAAACACTATTTTTCCCTAAGCAGCACTGATAAAATCAAATCTACTATGTCATAACATAATTTATGTAA
CATTTTAAAAGTCGGGCTTC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLE
ELAQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYID
ITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEF
LQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAE
LRDKVQEDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI

Sequences:

>Translated_719_residues
MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLE
ELAQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYID
ITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEF
LQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAE
LRDKVQEDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI
>Mature_718_residues
GLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEE
LAQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNA
GRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSNQ
LGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDI
TEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFL
QMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYGS
PESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAEL
RDKVQEDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDV
YAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=707, Percent_Identity=24.1867043847242, Blast_Score=154, Evalue=4e-37,
Organism=Escherichia coli, GI1789030, Length=718, Percent_Identity=48.8857938718663, Blast_Score=721, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=693, Percent_Identity=24.2424242424242, Blast_Score=156, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17555242, Length=630, Percent_Identity=25.0793650793651, Blast_Score=163, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322123, Length=661, Percent_Identity=24.0544629349471, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320914, Length=669, Percent_Identity=23.168908819133, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17352481, Length=519, Percent_Identity=25.8188824662813, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81593; Mature: 81462

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYL
CCCCHHCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLENM
HHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCEECCC
EDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNAGRARRGELVSCFLIQVTDD
CCEEEEEEEEEECCCCEEEEEEHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECCH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYSVEKEYGVPFNYIDITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYV
EEECCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEE
VNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFLQMGTDVSCNLGSTNVVNMM
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEC
TSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
CCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCC
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYANGSYFDKYVTGEFVPTS
CCCCCHHHHHHHHHHHHHHHEECCCCHHHCCCEEECCCCCCCCCCCEEHHHCCCCCCCCH
DRVKELFKNVFIPGVADWAELRDKVQEDGLYHQNRLAVAPNGSISYINDVSASIHPITQR
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCHHHHHHHH
IEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHCCC
>Mature Secondary Structure 
GLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYL
CCCHHCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLENM
HHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCEECCC
EDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNAGRARRGELVSCFLIQVTDD
CCEEEEEEEEEECCCCEEEEEEHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECCH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYSVEKEYGVPFNYIDITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYV
EEECCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEE
VNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFLQMGTDVSCNLGSTNVVNMM
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEC
TSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
CCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCC
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYANGSYFDKYVTGEFVPTS
CCCCCHHHHHHHHHHHHHHHEECCCCHHHCCCEEECCCCCCCCCCCEEHHHCCCCCCCCH
DRVKELFKNVFIPGVADWAELRDKVQEDGLYHQNRLAVAPNGSISYINDVSASIHPITQR
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCHHHHHHHH
IEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]