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The map label for this gene is nrdF [H]

Identifier: 225860977

GI number: 225860977

Start: 982809

End: 983771

Strand: Reverse

Name: nrdF [H]

Synonym: SPT_1043

Alternate gene names: 225860977

Gene position: 983771-982809 (Counterclockwise)

Preceding gene: 225860978

Following gene: 225860976

Centisome position: 46.58

GC content: 41.02

Gene sequence:

>963_bases
ATGGAAACTTACTACAAAGCCATTAACTGGAATGCCATCGAAGATGTCATCGACAAATCAACTTGGGAAAAACTGACGGA
GCAATTTTGGCTCGATACACGTATTCCCTTATCAAACGACTTGGATGACTGGAGAAAGCTATCTAACAAAGAGAAAGACT
TGGTCGGAAAAGTTTTTGGTGGTTTAACCCTTCTTGACACTATGCAATCTGAAACTGGGGTTCAAGCCCTTCGCGCAGAC
ATCCGTACACCACATGAGGAAGCTGTTTTCAATAACATCCAATTTATGGAATCTGTCCACGCTAAATCTTACTCATCAAT
CTTTTCTACCTTGAATACTAAGGCTGAGATTGAAGAAATTTTCGAATGGACCAATACCAATCCTTACCTACAAAAGAAGG
CTGAGATTGTCAACGAAATCTACCTAAACGGCAGCCCACTTGAAAAGAAAGTTGCCAGCGTCTTCCTCGAAACCTTCCTC
TTCTACTCTGGTTTCTTCACTCCCCTCTACTATCTCGGTAACAACAAACTTGCCAACGTTGCAGAAATCATTAAATTGAT
TATTCGTGACGAGTCTGTTCACGGAACCTACATTGGTTACAAATTCCAACTTGGTTTCAATGAATTACCTGAAGAAGAGC
AAGAAAAACTCAAAGAATGGATGTACGACCTACTCTATACTCTTTATGAAAATGAAGAAGGCTACACAGAGAGTCTTTAT
GACGGTGTTGGTTGGACTGAAGAGGTTAAAACCTTCCTTCGCTACAATGCCAATAAAGCTCTCATGAACATGGGACAAGA
TCCACTCTTCCCAGATTCAGCTGATGATGTCAACCCAATCGTAATGAACGGTATTTCAACAGGAACTTCCAACCACGACT
TCTTCTCTCAAGTCGGAAATGGTTACCTTCTTGGCGAAGTTGAAGTCATGCAAGACGATGACTATAACTACGGTTTAGAC
TAA

Upstream 100 bases:

>100_bases
AAAGTCGGGCTTCCGACTTTTTGTGCGATACTCCTATAGAATTATGGTAAAGTAGAAATAATTGTGAGTTCGCAATACTA
AACACAGAAAGAGATTTTAA

Downstream 100 bases:

>100_bases
TTCTCCACCACTCTCAATCATCAAAAAGTAAACTAGAAACATCAAAGTTATAACCCAACAAAAATATCGAAAGTTTTGAA
AACAAACTTCGATATTTTTG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRAD
IRTPHEEAVFNNIQFMESVHAKSYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLKEWMYDLLYTLYENEEGYTESLY
DGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADDVNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD

Sequences:

>Translated_320_residues
METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRAD
IRTPHEEAVFNNIQFMESVHAKSYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLKEWMYDLLYTLYENEEGYTESLY
DGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADDVNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD
>Mature_320_residues
METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRAD
IRTPHEEAVFNNIQFMESVHAKSYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLKEWMYDLLYTLYENEEGYTESLY
DGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADDVNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=254, Percent_Identity=24.4094488188976, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI289177074, Length=254, Percent_Identity=24.4094488188976, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789031, Length=312, Percent_Identity=50, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1788567, Length=346, Percent_Identity=26.0115606936416, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36977; Mature: 36977

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSNKEKDLVGKVFG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
GLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAKSYSSIFSTLNTKAEIEEI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFLFYSGFFTPLYYLGNNKLANV
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLKEWMYDLLYTLYENEEGYTESLY
HHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADDVNPIVMNGISTGTSNHDFFSQVGN
HCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHCCC
GYLLGEVEVMQDDDYNYGLD
CEEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSNKEKDLVGKVFG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
GLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAKSYSSIFSTLNTKAEIEEI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFLFYSGFFTPLYYLGNNKLANV
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLKEWMYDLLYTLYENEEGYTESLY
HHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADDVNPIVMNGISTGTSNHDFFSQVGN
HCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHCCC
GYLLGEVEVMQDDDYNYGLD
CEEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]