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The map label for this gene is lacR [H]

Identifier: 225860976

GI number: 225860976

Start: 981866

End: 982627

Strand: Reverse

Name: lacR [H]

Synonym: SPT_1042

Alternate gene names: 225860976

Gene position: 982627-981866 (Counterclockwise)

Preceding gene: 225860977

Following gene: 225860962

Centisome position: 46.52

GC content: 34.78

Gene sequence:

>762_bases
ATGCTCAAACAAGAAAAATTAACCAAAATTTTAGAGATAGTAAATAGCAAAGGAACCATAACGGTTAAACAGATTATGGA
CGAAATAGCCGTTTCAGATATGACTGCAAGGCGCTATTTACAGGAATTAGCTGATAAAGATTTGCTGATTCGTGTGCATG
GTGGAGCTGAAAAACTTCGAACCAACTCCCTTTTGACTAATGAGCGATCAAATATTGAAAAACAAGCCCTCCAAACGGCA
GAAAAACAAGAAATAGCCCATTTTGCAGGCAGTCTAGTAGAAGAAAGAGAAACTATTTTCATTGGACCAGGAACAACATT
AGAGTTTTTTGCGCGTGAGTTGCCTATTGACAATATCCGCGTCGTAACCAACAGTCTACCTGTTTTTCTGATTTTAAGCG
AACGAAAATTAACAGATTTGATTTTAATAGGTGGAAATTATCGCGATATTACAGGTGCTTTTGTTGGCACATTGACCCTA
CAAAATCTCTCTAATCTCCAATTTTCTAAAGCTTTCGTTAGCTGTAATGGTATTCAAAACGGAGCTCTAGCTACTTTTAG
CGAGGAAGAGGGAGAGGCTCAACGCATCGCTTTAAATAATTCTAATAAAAAATATTTACTCGCAGATCATAGTAAGTTCA
ATAAGTTTGATTTTTATACTTTTTATAATATATCAAATCTTGATACTATTGTTTCAGATTCTAAACTAAGTGATTCAATC
CTTTTTAAGCTATCTAAACACATTAAAGTCATCAAGCCTTAA

Upstream 100 bases:

>100_bases
ACAAACTTCGATATTTTTGTTTTTGTTTCATTTTGTTGTTTTTGTATTTGAATGATGAGTTTGTTTATGGTAAGATAGTA
GTAGGAAACGAGGTGATAAT

Downstream 100 bases:

>100_bases
TAAACTGAAAGAGCCTGGGTAACAACTCAATTTCAGAAGAAAAAAAGTACATCTTCCCACAATAAAACGCATAGTATCAG
GTGTTGAAACACCTGATACT

Product: lactose phosphotransferase system repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELADKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTA
EKQEIAHFAGSLVEERETIFIGPGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDFYTFYNISNLDTIVSDSKLSDSI
LFKLSKHIKVIKP

Sequences:

>Translated_253_residues
MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELADKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTA
EKQEIAHFAGSLVEERETIFIGPGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDFYTFYNISNLDTIVSDSKLSDSI
LFKLSKHIKVIKP
>Mature_253_residues
MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELADKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTA
EKQEIAHFAGSLVEERETIFIGPGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDFYTFYNISNLDTIVSDSKLSDSI
LFKLSKHIKVIKP

Specific function: Repressor of the lactose catabolism operon. Galactose-6- phosphate is the inducer [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789829, Length=249, Percent_Identity=31.7269076305221, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI226510968, Length=248, Percent_Identity=27.4193548387097, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787540, Length=238, Percent_Identity=30.2521008403361, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87082344, Length=241, Percent_Identity=26.5560165975104, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789170, Length=235, Percent_Identity=27.2340425531915, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787063, Length=258, Percent_Identity=27.906976744186, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1790753, Length=247, Percent_Identity=26.7206477732794, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789519, Length=245, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789059, Length=242, Percent_Identity=26.4462809917355, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790635, Length=250, Percent_Identity=25.2, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788069, Length=255, Percent_Identity=24.3137254901961, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 28432; Mature: 28432

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELADKDLLIRVHGGAEKLR
CCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
TNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIGPGTTLEFFARELPIDNIR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEE
VVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTLQNLSNLQFSKAFVSCNGIQN
EEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCC
GALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDFYTFYNISNLDTIVSDSKLSDSI
CCEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEEEECCCCHHHHCCCCCHHHH
LFKLSKHIKVIKP
HHHHHHCEEEECC
>Mature Secondary Structure
MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELADKDLLIRVHGGAEKLR
CCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
TNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIGPGTTLEFFARELPIDNIR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEE
VVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTLQNLSNLQFSKAFVSCNGIQN
EEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCC
GALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDFYTFYNISNLDTIVSDSKLSDSI
CCEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEEEECCCCHHHHCCCCCHHHH
LFKLSKHIKVIKP
HHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120234; 1901863 [H]