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The map label for this gene is thiD [H]

Identifier: 225860704

GI number: 225860704

Start: 706245

End: 707036

Strand: Reverse

Name: thiD [H]

Synonym: SPT_0742

Alternate gene names: 225860704

Gene position: 707036-706245 (Counterclockwise)

Preceding gene: 225860710

Following gene: 225860689

Centisome position: 33.47

GC content: 43.06

Gene sequence:

>792_bases
ATGACTTATTTACCCGTTGCTTTGACCATTGCAGGGACTGACCCTAGTGGTGGTGCTGGCATTATGGCAGATTTAAAGTC
ATTCCAAGCGAGAGATGTCTATGGAATGGCTGTTGTAACCAGTCTTGTCGCTCAAAATACCAGAGGTGTTCAGCTAATCG
AGCACGTTTCTCCTCAAATGTTGAAAGCCCAATTGGAGAGTGTCTTTTCTGATATTCCACCTCAGGCTGTAAAAACTGGA
ATGTTGGCTACTACTGAAATCATGGAAATCATCCAACCCTATCTTAAAAAACTGGATTGTCCCTATGTCCTTGATCCTGT
TATGGTTGCTACAAGTGGAGATGCCTTGATTGACTCAAATGCTAGAGACTATCTCAAAACAAACTTACTACCTCTAGCAA
CTATTATTACGCCAAATCTTCCTGAAGCAGAAGAGATTGTTGGTTTTTCAATCCATGACCCCGAAGACATGCAGCGTGCT
GGTCGCCTGATTTTAAAAGAATTTGGTCCTCAGTCTGTGGTTATCAAAGGCGGACATCTCAAAGGTGGTGCTAAAGATTT
CCTCTTTACCAAGAATGAACAATTTGTCTGGGAAAGCCCACGAATTCAAACCTGTCACACCCATGGTACTGGATGTACCT
TTGCTGCAGTGATTACTGCTGAACTAGCCAAGGGCAAGAGTCTTTACCAGGCAGTTGATAAGGCCAAGGCCTTTATCACA
AAAGCTATTCAAGATGCCCCTCAACTCGGTCATGGTTCTGGTCCAGTCAACCATACAACTTTTAAAGATTAA

Upstream 100 bases:

>100_bases
AGTGGAAATGATAATGGGGACTAGCAGTCTTCTATTGCCTTTCTAAAACAGACTAGCTTGTTCTTAAGAATACAAACTTC
AGTTGGTTGGGAGGTTTTAG

Downstream 100 bases:

>100_bases
GAAAAAAACTCTCTAGTTCCCACTTTAAGGGAATTAGAGAGTTTTTATACTCTTCGAAAATCTCTTCAAACTACGCCAGC
TTCCATCTGCAACTTCAAAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTG
MLATTEIMEIIQPYLKKLDCPYVLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFIT
KAIQDAPQLGHGSGPVNHTTFKD

Sequences:

>Translated_263_residues
MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTG
MLATTEIMEIIQPYLKKLDCPYVLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFIT
KAIQDAPQLGHGSGPVNHTTFKD
>Mature_262_residues
TYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGM
LATTEIMEIIQPYLKKLDCPYVLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRAG
RLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFITK
AIQDAPQLGHGSGPVNHTTFKD

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=45.136186770428, Blast_Score=206, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=30.6273062730627, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=34.5588235294118, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=33.8235294117647, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28336; Mature: 28205

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSPQM
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCHHH
LKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPYVLDPVMVATSGDALIDSN
HHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCEECCC
ARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRAGRLILKEFGPQSVVIKGGHL
CHHHHHHCCHHHHHHHCCCCCCHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
KGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFIT
CCCCCCCEEECCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KAIQDAPQLGHGSGPVNHTTFKD
HHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSPQM
CEEEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCHHH
LKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPYVLDPVMVATSGDALIDSN
HHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCEECCC
ARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRAGRLILKEFGPQSVVIKGGHL
CHHHHHHCCHHHHHHHCCCCCCHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
KGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFIT
CCCCCCCEEECCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KAIQDAPQLGHGSGPVNHTTFKD
HHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]