Click here to switch to the map view.

The map label for this gene is hsdR [H]

Identifier: 225860526

GI number: 225860526

Start: 513836

End: 516169

Strand: Reverse

Name: hsdR [H]

Synonym: SPT_0545

Alternate gene names: 225860526

Gene position: 516169-513836 (Counterclockwise)

Preceding gene: 225860527

Following gene: 225860525

Centisome position: 24.44

GC content: 36.8

Gene sequence:

>2334_bases
ATGGTTGAGTTTATAAAGTCTAAGAAAGAAATGAGTGAGGAGGATATTAAAGCAAATTTCATCACTCCTGCTATTGTATC
CAAAGGATGGAAAAATGGTGAGCATATCGCTTACGAAGAATACTTCACTGATGGTCGAATTGAGGTTAGAGGAGATAAGG
CTCGTCGTAAAGAAGGAAAAAAATCAGACTATTCACTGTATTACCAATTTGGAACTCGAATTGCAATTGTTGAGGCAAAG
GATAATAAACACAGCGTTCGAGCAGGATTACAACAAGCTATTGAATATGGAGAGATTTTAGATGTTCCATTTGTTTATTC
TTCAAATGGTGATGGCTTTATTGAACACGACCGTATCACGAGAGAAGAACGTGAGCTGGAGTTAGACGAATTCCCTACTC
GTGAAGAATTATTTTCTCGTATGACGAAGGAAAAAGGATTGACGTACGAAATTACAGAAGCTATCTCAACTCCATACTAT
ACAGACGCCTTCTCAATGAAAACGCCACGCTATTATCAGCAAATAGCTATCAACCGTACTATTGAAACAGTTGCCAGAGG
ACAAAAACGAGTAATGTTTGTGATGGCAACAGGAACGGGGAAAACGTTCATGGCTTTTCAAATTATTCATCGCCTTCGAA
AAGCTGGTTTGGCTAAACGAGTTTTATTCTTAGCGGATAGAAACATCTTAGTAGACCAAACGATGGCTGAAGACTTTAAG
CCATTCGAAAAGGTAATGACGAAAATTACACCAAAACTTTTGACTGCTCCTGAAAAATTAAATTCTTTTGAAATTTATCT
AGGGCTTTATCAGCAACTAACTGGTGAAGATGGAACTGAAACACATTATCAAAAATTTGACAAAGACTTCTTTGATTTAA
TCGTAATTGATGAAGCGCACCGTGGTTCAGCTAAGGAAAACAGTAACTGGCGTAAGATAATTGATTATTTCAGTTCTGCG
ACACAGATTGGGATGACCGCTACTCCTAAAGAAACCAAGAATGCTTCCAATACGGAATACTTTGGTGAGCCAATCTATAC
TTATAGTTTAAAACAGGGAATCGAGGATGGTTTTTTGGCTCCATATCGTGTTATGAGGGTTAATTTAGATGTGGATGTGG
ATGGTTATCGTCCAGAAACTGGAAAAGTTGATACTAACGGACAATTAATAGAAGATAGGTACTACGGCAGGAAAGATTTT
GATAAAACCATTGTCATTGATGATAGAACGCAAAGAGTTGCCAAGTTTGTTTCTGATTATATGAAGCAAAGCAATGCACG
ATTTGATAAAACAATTGTTTTTTGTGTTGATATTGACCATGCCGAGCGAATGCGTGCTGCACTTGTAAAAGAGAATCTAG
ACTTAGTCCAAGAAGACTATCGTTATGTCATGCAAGTAACTGGTGACAACGCTGAAGGAAAAGCTCAACTGGATAACTTT
ATGGATGTCAATTCTAATTTTCCCGCTATTGTAACAACGTCTAAATTATTAACGACAGGAGTTAATGCTAAAACATGTCG
TTTGATTGTTTTAGACTCTAATATCCAATCCATGACTGAATTTAAACAAATTATTGGTCGTGGCACACGTCTTTATCCTC
AAAAGGGGAAAGAATTTTTTACGATTATTGATTTTCGAAATGTTACCAATTTGTTTGCTGACCCTGATTTTGATGGTGAT
CCTGTGAAGGTGCTGGAAACAGGTGCGAAAACAGTCAGTGGTTCTACGCCCGGTTTCGTAGATGAGGAAGGTGACCCAGT
AGAAAAATATATCGTTACAGACAAGCAGGTTACCATTCTTAATTCTACTGTTCAAGTATTAGATGAAAACGGGAAACTGA
TTACCGAAAGCCTGACCGACTACACTCGGAAGAATATCTTAGGTAGCTACGCCACTTTGAACGATTTTATCACAGTTTGG
CATACGGCGGATAAAAAGAAGCTTATCTTAGACGAACTTTATAAAAAAGGAGTTTATCTAGATGCTATTCGAGAGTCGGA
GGGAATATCAGAACAAGAAATCGATGATTTTGATTTACTCCTAAAACTTGCCTATGGTCAAAAAGAATTAACCAAAACGG
AACGTATCAATAAACTCAAACAAAGCGGATATTTATATAAATATAGTGAGGAAGCGCGTGCTGTTTTGGAAATTTTACTG
AACAAATACATGGATAAAGGTATTGGAGAACTCGAAAGCATTGAAACATTAAAACTTCCAGAATTTCAGATATATGGTGG
AACCTTCAAAATCATCAATACTTATTTTGGAGATAAAAAACGATATTTACAAGCAATTAAAGAATTGGAGCAAGAGCTAT
TTACAGTAGCTTAA

Upstream 100 bases:

>100_bases
TTAGCAGAGTATTTTTATAAGTGTGATATAATAGAAGTATAATTTGTTCTGATAGTTTATTTTATGGAGAAGTAGATTTT
TAGAATGCGGAGGGTTCAAT

Downstream 100 bases:

>100_bases
TGAAAGGAAAGTATGTCAATTACATCATTTGTAAAAAGAATTCAAGATATCACTCGAAACGATGCTGGTGTTAATGGTGA
TGCTCAACGTATTGAGCAAA

Product: type I restriction-modification system, R subunit

Products: NA

Alternate protein names: R.EcoAI [H]

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTDGRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAK
DNKHSVRAGLQQAIEYGEILDVPFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLAKRVLFLADRNILVDQTMAEDFK
PFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSA
TQIGMTATPKETKNASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIEDRYYGRKDF
DKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVKENLDLVQEDYRYVMQVTGDNAEGKAQLDNF
MDVNSNFPAIVTTSKLLTTGVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGD
PVKVLETGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRKNILGSYATLNDFITVW
HTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLKLAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILL
NKYMDKGIGELESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA

Sequences:

>Translated_777_residues
MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTDGRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAK
DNKHSVRAGLQQAIEYGEILDVPFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLAKRVLFLADRNILVDQTMAEDFK
PFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSA
TQIGMTATPKETKNASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIEDRYYGRKDF
DKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVKENLDLVQEDYRYVMQVTGDNAEGKAQLDNF
MDVNSNFPAIVTTSKLLTTGVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGD
PVKVLETGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRKNILGSYATLNDFITVW
HTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLKLAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILL
NKYMDKGIGELESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA
>Mature_777_residues
MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTDGRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAK
DNKHSVRAGLQQAIEYGEILDVPFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLAKRVLFLADRNILVDQTMAEDFK
PFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSA
TQIGMTATPKETKNASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIEDRYYGRKDF
DKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVKENLDLVQEDYRYVMQVTGDNAEGKAQLDNF
MDVNSNFPAIVTTSKLLTTGVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGD
PVKVLETGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRKNILGSYATLNDFITVW
HTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLKLAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILL
NKYMDKGIGELESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA

Specific function: The EcoAI enzyme recognizes 5'-GAGN(7)GTCA-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=575, Percent_Identity=28, Blast_Score=153, Evalue=5e-38,
Organism=Escherichia coli, GI1788511, Length=407, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 89295; Mature: 89295

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTDGRIEVRGDKARRKEGK
CCHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCEEEEHHHCCCCEEEECCCHHHHCCCC
KSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDVPFVYSSNGDGFIEHDRIT
CCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHCCEECCCEEEECCCCCCEECCHHC
REERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYYTDAFSMKTPRYYQQIAINRT
CHHHHCCHHCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
IETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLAKRVLFLADRNILVDQTMAEDFK
HHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEHHHHHHHH
PFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAH
HHHHHHHHHCHHHHCCHHHCCCEEEHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCC
RGSAKENSNWRKIIDYFSSATQIGMTATPKETKNASNTEYFGEPIYTYSLKQGIEDGFLA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEHHHHCCCCCCCC
PYRVMRVNLDVDVDGYRPETGKVDTNGQLIEDRYYGRKDFDKTIVIDDRTQRVAKFVSDY
CEEEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCHHHHHHHHHHHH
MKQSNARFDKTIVFCVDIDHAERMRAALVKENLDLVQEDYRYVMQVTGDNAEGKAQLDNF
HHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
MDVNSNFPAIVTTSKLLTTGVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFF
HCCCCCCCEEEEEHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEECCCCCCEEE
TIIDFRNVTNLFADPDFDGDPVKVLETGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTIL
EEEEHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCEEEEE
NSTVQVLDENGKLITESLTDYTRKNILGSYATLNDFITVWHTADKKKLILDELYKKGVYL
HHHHHHHCCCCCEEHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCHHHHHHHHHHCCCEE
DAIRESEGISEQEIDDFDLLLKLAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILL
EEHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHH
NKYMDKGIGELESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA
HHHHHCCCCCHHCCCEECCCCEEEECCEEEEEHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTDGRIEVRGDKARRKEGK
CCHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCEEEEHHHCCCCEEEECCCHHHHCCCC
KSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDVPFVYSSNGDGFIEHDRIT
CCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHCCEECCCEEEECCCCCCEECCHHC
REERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYYTDAFSMKTPRYYQQIAINRT
CHHHHCCHHCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
IETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLAKRVLFLADRNILVDQTMAEDFK
HHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEHHHHHHHH
PFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAH
HHHHHHHHHCHHHHCCHHHCCCEEEHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCC
RGSAKENSNWRKIIDYFSSATQIGMTATPKETKNASNTEYFGEPIYTYSLKQGIEDGFLA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEHHHHCCCCCCCC
PYRVMRVNLDVDVDGYRPETGKVDTNGQLIEDRYYGRKDFDKTIVIDDRTQRVAKFVSDY
CEEEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCHHHHHHHHHHHH
MKQSNARFDKTIVFCVDIDHAERMRAALVKENLDLVQEDYRYVMQVTGDNAEGKAQLDNF
HHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
MDVNSNFPAIVTTSKLLTTGVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFF
HCCCCCCCEEEEEHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEECCCCCCEEE
TIIDFRNVTNLFADPDFDGDPVKVLETGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTIL
EEEEHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCEEEEE
NSTVQVLDENGKLITESLTDYTRKNILGSYATLNDFITVWHTADKKKLILDELYKKGVYL
HHHHHHHCCCCCEEHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCHHHHHHHHHHCCCEE
DAIRESEGISEQEIDDFDLLLKLAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILL
EEHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHH
NKYMDKGIGELESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA
HHHHHCCCCCHHCCCEECCCCEEEECCEEEEEHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]