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The map label for this gene is hsdM [H]

Identifier: 225860525

GI number: 225860525

Start: 512360

End: 513823

Strand: Reverse

Name: hsdM [H]

Synonym: SPT_0544

Alternate gene names: 225860525

Gene position: 513823-512360 (Counterclockwise)

Preceding gene: 225860526

Following gene: 225860524

Centisome position: 24.33

GC content: 34.77

Gene sequence:

>1464_bases
ATGTCAATTACATCATTTGTAAAAAGAATTCAAGATATCACTCGAAACGATGCTGGTGTTAATGGTGATGCTCAACGTAT
TGAGCAAATGTCTTGGTTATTATTCTTAAAAATTTATGATAGCCGTGAAATGGTTTGGGAATTAGAAGAAGACGAGTATG
AGTCAATTATCCCAGAGGAATTAAAATGGCGAAATTGGGCTCATGCTCAAAATGGGGAACGGGTATTGACAGGCGATGAA
TTACTTGATTTTGTCAATAACAAGTTATTCAAAGAGTTGAAAGAGCTTGAAATAACTTCAAATATGCCTATTCGAAAAAC
GATTGTTAAATCAGCTTTTGAAGATGCGAACAACTATATGAAAAATGGCGTCTTGTTACGCCAAGTCATCAATGTTATTG
ATGAAGTTGATTTCAATAGCCCTGAAGATCGTCATTCGTTTAATGATATTTACGAAAAAATTCTTAAAGATATTCAAAAT
GCTGGGAACTCAGGAGAATTTTATACGCCACGTGCAGCGACTGATTTTATTGCCGAAGTTCTTAACCCAAAACTTGGAGA
ATCAATGGCAGACCTTGCTTGCGGAACAGGAGGCTTCTTGACTTCGACTCTGAACCGTTTAAGTAGTCAACGTAAAACTA
GTGAAGATACCAAAAAATATAATACAGCTGTTTTTGGTATTGAAAAGAAAGCATTTCCTCATCTTTTAGCAGTTACAAAT
CTGTTTCTTCACGAAATTGATGACCCTAAAATTGTTCATGGAAATACTTTGGAGAAAAATGTTCGTGAATATACGGATGA
TGAAAAATTTGACATTATTATGATGAATCCACCTTTTGGAGGGTCAGAATTAGAAACAATAAAAAATAACTTTCCAGCAG
AATTACGGAGTTCTGAAACAGCTGATTTATTTATGGCTGTCATTATGTATCGTTTGAAAGAAAATGGTCGTGTTGGAGTT
ATTTTACCTGATGGTTTTCTATTTGGTGAAGGTGTAAAAACTCGCTTGAAACAAAAACTGGTAGATGAGTTCAACTTGCA
TACGATTATTAGGTTGCCTCATAGTGTCTTTGCACCGTATACAGGAATCCATACGAACATTCTTTTCTTTGATAAAACAA
AGAAAACAGAAGAAACTTGGTTTTATCGTTTAGATATGCCAGATGGTTATAAAAATTTCTCGAAAACTAAGCCGATGAAG
TCAGAACACTTCAATCCTGTTCGTGACTGGTGGGAAAATCGTGAAGAGATTCTGGAAGGTAAGTTCTACAAATCTAAATC
ATTTACACCTAGTGAATTGGCTGAGTTGAATTATAATTTAGACCAGTGTGGCTTTCCAAAAGAGGAAGAGGAAATCTTAG
ATCCCTTTGAGTTGATTCAGAATTATCAAGCGGAAAGAGCAACTTTAAATCATAAGATTGATAATGTATTAGCTGATATT
TTGCAGTTGTTGGAGGACAAATAA

Upstream 100 bases:

>100_bases
CAAAATCATCAATACTTATTTTGGAGATAAAAAACGATATTTACAAGCAATTAAAGAATTGGAGCAAGAGCTATTTACAG
TAGCTTAATGAAAGGAAAGT

Downstream 100 bases:

>100_bases
TGACACCAGAACAACTTAAAGCAAGTATTCTCCAAAGAGCGATGGAAGGGAAATTAGTGCCGCAAAATCCCAATGACGAA
CCTGCAAGTGAATTATTAAA

Product: type I restriction enzyme

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREMVWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDE
LLDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTKKYNTAVFGIEKKAFPHLLAVTN
LFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGV
ILPDGFLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKNFSKTKPMK
SEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEEEILDPFELIQNYQAERATLNHKIDNVLADI
LQLLEDK

Sequences:

>Translated_487_residues
MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREMVWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDE
LLDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTKKYNTAVFGIEKKAFPHLLAVTN
LFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGV
ILPDGFLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKNFSKTKPMK
SEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEEEILDPFELIQNYQAERATLNHKIDNVLADI
LQLLEDK
>Mature_486_residues
SITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREMVWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDEL
LDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQNA
GNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTKKYNTAVFGIEKKAFPHLLAVTNL
FLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVI
LPDGFLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKNFSKTKPMKS
EHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEEEILDPFELIQNYQAERATLNHKIDNVLADIL
QLLEDK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=445, Percent_Identity=27.6404494382022, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56482; Mature: 56350

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREMVWELEEDEYESIIPEE
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCHHEEECCCHHHHHCCCHH
LKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYM
HHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQNAGNSGEFYTPRAATDFIAEV
HCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHH
LNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTKKYNTAVFGIEKKAFPHLLAVTN
HCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCHHHHHHHHHHHH
LFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGSELETIKNNFPAELRSSET
HHHHHCCCCCEECCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCHHHCCCCH
ADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPY
HHHHHHHHHHHHHCCCCEEEEECCCCEECCHHHHHHHHHHHHHHCCHHHHHCCHHHHCCC
TGIHTNILFFDKTKKTEETWFYRLDMPDGYKNFSKTKPMKSEHFNPVRDWWENREEILEG
CCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHCCCCCHHCCCHHHHHHHCHHHHHCC
KFYKSKSFTPSELAELNYNLDQCGFPKEEEEILDPFELIQNYQAERATLNHKIDNVLADI
CHHCCCCCCHHHHHHHCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLLEDK
HHHHCCC
>Mature Secondary Structure 
SITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREMVWELEEDEYESIIPEE
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCHHEEECCCHHHHHCCCHH
LKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYM
HHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQNAGNSGEFYTPRAATDFIAEV
HCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHH
LNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTKKYNTAVFGIEKKAFPHLLAVTN
HCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCHHHHHHHHHHHH
LFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGSELETIKNNFPAELRSSET
HHHHHCCCCCEECCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCHHHCCCCH
ADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPY
HHHHHHHHHHHHHCCCCEEEEECCCCEECCHHHHHHHHHHHHHHCCHHHHHCCHHHHCCC
TGIHTNILFFDKTKKTEETWFYRLDMPDGYKNFSKTKPMKSEHFNPVRDWWENREEILEG
CCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHCCCCCHHCCCHHHHHHHCHHHHHCC
KFYKSKSFTPSELAELNYNLDQCGFPKEEEEILDPFELIQNYQAERATLNHKIDNVLADI
CHHCCCCCCHHHHHHHCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLLEDK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]