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The map label for this gene is hsdS [H]

Identifier: 225860524

GI number: 225860524

Start: 510795

End: 512360

Strand: Reverse

Name: hsdS [H]

Synonym: SPT_0542

Alternate gene names: 225860524

Gene position: 512360-510795 (Counterclockwise)

Preceding gene: 225860525

Following gene: 225860521

Centisome position: 24.26

GC content: 31.61

Gene sequence:

>1566_bases
ATGACACCAGAACAACTTAAAGCAAGTATTCTCCAAAGAGCGATGGAAGGGAAATTAGTGCCGCAAAATCCCAATGACGA
ACCTGCAAGTGAATTATTAAAGAGAATTAAAGCTGAAAAAGAAAAACTTATCAGTGAAGGAAAAATCAAACGAGATAAAA
AGGAAACTGAGATATTTCGTGGTGATGATGGGAAACATTATGGGAAGTTTGCTGATGGAAGCACTCAAGAAATTGATGTT
CCTTATGATATTCCTGATACTTGGGAGTGGGTGAGGTTTTCTACATTGGTTGAAATTGTCAGAGGTGGCTCTCCACGACC
AATCAAAGATTATCTTACTTCTGAAGTAGATGGAATAAATTGGATAAAAATAGGTGATACTGAAAAGGGTGAAAAGTATA
TAAATAATGTTAAAGAAAAAATCAAAAAATCAGGGCTTAACAAAACTAGATTTGTAAAAAAAGGTACATTTTTGTTAACT
AATTCTATGAGTTTTGGTAGACCTTATATTTTGAATGTTGATGGTGCAATACACGATGGATGGTTGGCTATTTCGAACTA
TGAAAACTCATTAAATAAAGATTACCTATTCTATATTCTTTCATCAAATGTAGTTTATTCTCAATTTCTATCTCTAATTA
GTGGAGCTGTTGTGAAAAACTTGAATAGTGATAAAGTTGCTTCTATTCTTATCCCTCTCCCTCCGCTAGCTGAACAACAA
CGCATAATAGAAGCAATCGAATCAGCTTTAGAAAAAGTAGATGAATATGCTGAAAGTTATAATAGACTAGAACAGCTAGA
TAAAGAATTTCCAGATAAACTAAAAAAATCTATTCTTCAATATGCTATGCAAGGAAAATTAGTTGAACAAGACCCAAATG
ATGAATCAGTCGAAGTTTTACTTGAAAAAATACGAGCAGAAAAACAAAAACTCTTTGAAGAAGGCAAGATTAAAAAGAAA
GATTTGGACATTTCTATTGTTTCCCAAGGAGATGATAACTCTTATTATGGGAATATACCTATGAATTGGGTTGTTATAAA
AATAAAAGATATTTTTTCAATGAATACAGGTCTTTCTTACAAGAAGGGCGATTTAAGCATTAATAATAAAGGTGTTAGAA
TTATACGTGGTGGTAATATTAAGCCTTTAGAATTTTCTCTGTTGGATAATGATTACTACATTGATACACAATTCATCTCC
TCTGAGCAAGTTTATTTAAAACATAATCAGCTAATAACACCTGTAGCAACCTCTTTAGAACATATTGGAAAGTTTGCAAG
AATCGATAAAGACTATGATGGTGTTGTGGCTGGTGGATTTATTTTCCAATTAACACCATTCGAAAGTTCAGAGATTATTT
CAAAATTTCTATTATTTAACTTGTCCTCTCCGTTATTTTATAAACAATTGAAAGCAATAACTAAACTATCAGGTCAAGCT
TTATATAATATTCCTAAAACTACACTAAGCGAGCTATTAATTCCGTTAGCTCCTTTTGAGGAACAGGAACTTATTACTCA
AAAAGTTGAGAAACTTTTTGAAAAAGTAAATCAACTTTGGAAATGA

Upstream 100 bases:

>100_bases
CCTTTGAGTTGATTCAGAATTATCAAGCGGAAAGAGCAACTTTAAATCATAAGATTGATAATGTATTAGCTGATATTTTG
CAGTTGTTGGAGGACAAATA

Downstream 100 bases:

>100_bases
TTCTTTTCATCTCTTCATGATTAGAAATAGGGATTAATAATTCGGAGATACTGGTACTATTTAATGTTTTCCCTTTGATA
GCATCTTTTGAATCACCTAA

Product: type I site-specific deoxyribonuclease chain S

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQ
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKK
DLDISIVSQGDDNSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFIS
SEQVYLKHNQLITPVATSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK

Sequences:

>Translated_521_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQ
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKK
DLDISIVSQGDDNSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFIS
SEQVYLKHNQLITPVATSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK
>Mature_520_residues
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVP
YDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTN
SMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQR
IIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKD
LDISIVSQGDDNSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISS
EQVYLKHNQLITPVATSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQAL
YNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 59380; Mature: 59249

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEE
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGIN
CCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCE
WIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDG
EEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCEEEECCCCCCCCEEEECCCCCCCC
WLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQ
EEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHH
LEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYGNIPMNWVVIKIKDIFSMNTGLSY
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEECCCCCEEEEEEEEHHHHCCCCCCC
KKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLE
CCCCEEECCCCEEEEECCCCCCEEEEEECCCEEEEEEEECCCEEEEECCCCHHHHHHHHH
HIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
HHHHHHHCCCCCCCEEECCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK
EECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEE
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGIN
CCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCE
WIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDG
EEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCEEEECCCCCCCCEEEECCCCCCCC
WLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQ
EEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHH
LEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYGNIPMNWVVIKIKDIFSMNTGLSY
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEECCCCCEEEEEEEEHHHHCCCCCCC
KKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLE
CCCCEEECCCCEEEEECCCCCCEEEEEECCCEEEEEEEECCCEEEEECCCCHHHHHHHHH
HIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
HHHHHHHCCCCCCCEEECCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK
EECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]