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The map label for this gene is gatB [H]

Identifier: 225860459

GI number: 225860459

Start: 434201

End: 435643

Strand: Reverse

Name: gatB [H]

Synonym: SPT_0473

Alternate gene names: 225860459

Gene position: 435643-434201 (Counterclockwise)

Preceding gene: 225860460

Following gene: 225860458

Centisome position: 20.63

GC content: 45.81

Gene sequence:

>1443_bases
ATGAACTTTGAAACAGTCATCGGACTTGAAGTCCACGTAGAGCTCAACACCAATTCAAAAATCTTCTCACCTACTTCTGC
CCACTTCGGAAATGATCAAAATGCCAACACTAACGTGATTGACTGGTCTTTCCCAGGAGTTCTGCCAGTTCTCAATAAAG
GGGTTGTCGATGCCGGAATCAAGGCTGCTCTTGCCCTCAACATGGACATCCACAAAAAGATGCACTTTGACCGCAAGAAC
TACTTCTACCCTGATAATCCCAAAGCCTACCAAATTTCTCAGTTTGATGAGCCAATCGGTTATAACGGCTGGATTGAAGT
CGAACTAGAAGACGGTACGACCAAGAAAATCGGTATCGAACGTGCCCACCTAGAGGAAGACGCTGGTAAAAACACCCATG
GTACAGATGGCTACTCTTATGTTGACCTCAACCGCCAAGGGGTTCCCTTGATTGAGATTGTATCTGAGGCAGATATGCGT
TCTCCTGAAGAAGCCTATGCTTATCTGACAGCCCTCAAGGAAGTTATCCAGTACGCTGGCATTTCTGACGTTAAGATGGA
GGAAGGTTCGATGCGTGTGGATGCCAACATCTCCCTTCGTCCTTATGGTCAAGAGAAATTCGGTACCAAGACTGAATTGA
AGAACCTCAACTCCTTCTCAAACGTTCGTAAAGGTCTTGAATACGAAGTCCAACGCCAGGCTGAAATTCTTCGCTCAGGT
GGTCAAATCCGCCAAGAAACACGCCGTTACGATGAAGCGAATAAAGCAACCATCCTCATGCGTGTCAAGGAAGGGGCTGC
TGACTACCGCTACTTCCCAGAACCAGACCTACCCCTCTTTGAAATTTCTGACGAGTGGATTGAGGAAATGCGGACTGAGT
TGCCAGAGTTTCCAAAAGAACGTCGTGCGCGTTATGTATCTGACCTTGGTTTATCAGACTACGATGCTAGTCAGTTGACT
GCTAATAAAGTCACTTCTGACTTCTTTGAAAAAGCTGTTGCCCTAGGTGGTGATGCCAAACAAGTCTCTAACTGGCTCCA
AGGGGAAGTCGCTCAGTTCTTGAATGCTGAAGGTAAAACACTGGAACAAATCGAATTGACACCAGAAAACTTGGTTGAAA
TGATTGCCATCATCGAAGACGGTACTATTTCATCTAAGATTGCCAAGAAAGTCTTTGTCCATCTAGCTAAAAATGGCGGT
GGCGCGCGTGAATACGTGGAAAAAGCAGGTATGGTTCAAATTTCAGATCCAGCTGTTTTGATTCCAATTATCCACCAAGT
CTTTGCGGATAACGAAGCTGCTGTTGCCGACTTCAAGTCAGGCAAACGTAATGCAGACAAGGCCTTCACAGGATTCCTTA
TGAAGGCAACCAAAGGCCAAGCCAACCCACAAGTTGCCCTTAAACTACTTGCACAGGAATTGGCGAAGTTGAAAGAAAAC
TAG

Upstream 100 bases:

>100_bases
CCAAGCACTCTGAGGAAACCATTTACCAAGTTGCTGCTGCCTTTGAAGCAACAACAGGCTACCACAAACAACAACCCGTG
ATTTTTGGAGGTGACAACTA

Downstream 100 bases:

>100_bases
ACAGAACAAAACCAGCCCTAAGGTTGGTTTTTTCTTGACAAATAGAAAGAAAACGTTTGCAATATTGCGTGTAAAGTTTC
CAAACAAGAAAACTAAAAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
GQIRQETRRYDEANKATILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLT
ANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVFVHLAKNGG
GAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN

Sequences:

>Translated_480_residues
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
GQIRQETRRYDEANKATILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLT
ANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVFVHLAKNGG
GAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN
>Mature_480_residues
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
GQIRQETRRYDEANKATILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLT
ANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVFVHLAKNGG
GAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=34.5454545454545, Blast_Score=274, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=304, Percent_Identity=38.8157894736842, Blast_Score=208, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=29.2828685258964, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=32.258064516129, Blast_Score=250, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53645; Mature: 53645

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHCC
KAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIEVELEDGTTKKIGIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCH
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEVIQYAG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
CCCEEECCCCEEEECCEEECCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GQIRQETRRYDEANKATILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKE
CHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRARYVSDLGLSDYDASQLTANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
LEQIELTPENLVEMIAIIEDGTISSKIAKKVFVHLAKNGGGAREYVEKAGMVQISDPAVL
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCEEH
IPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHCC
KAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIEVELEDGTTKKIGIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCH
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEVIQYAG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
CCCEEECCCCEEEECCEEECCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GQIRQETRRYDEANKATILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKE
CHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRARYVSDLGLSDYDASQLTANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
LEQIELTPENLVEMIAIIEDGTISSKIAKKVFVHLAKNGGGAREYVEKAGMVQISDPAVL
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCEEH
IPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA