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The map label for this gene is gatA [H]

Identifier: 225860460

GI number: 225860460

Start: 435643

End: 437109

Strand: Reverse

Name: gatA [H]

Synonym: SPT_0474

Alternate gene names: 225860460

Gene position: 437109-435643 (Counterclockwise)

Preceding gene: 225860461

Following gene: 225860459

Centisome position: 20.69

GC content: 45.19

Gene sequence:

>1467_bases
ATGACTTTTAACAATAAAACTATTGAAGAGTTGCACAATCTCCTTGTCTCTAAGGAAATTTCTGCAACAGAATTGACCCA
AGCAACACTTGAAAATATCAAGTCTCGTGAGGAAGCCCTCAATTCATTTGTCACCATCGCTGAGGAGCAAGCTCTTGTTC
AAGCTAAAGCCATTGATGAAGCTGGAATTGATGCTGACAATGTCCTTTCAGGAATTCCACTTGCTGTTAAGGATAACATC
TCTACAGACGGTATTCTCACAACTGCTGCCTCAAAAATGCTCTACAACTATGAGCCAATCTTTGATGCGACAGCTGTTGC
CAATGCAAAAACCAAGGGCATGATTGTCGTTGGAAAGACCAACATGGACGAATTTGCTATGGGTGGTTCAGGTGAAACTT
CACACTACGGAGCAACTAAAAACGCTTGGGACCACAGCAAGGTTCCTGGTGGGTCATCAAGTGGTTCTGCCGCAGCTGTA
GCCTCAGGACAAGTTCGCTTGTCACTTGGTTCTGATACTGGTGGTTCCATCCGCCAACCTGCTGCCTTCAACGGAATCGT
TGGTCTCAAACCAACCTACGGAACAGTTTCACGTTTCGGTCTCATTGCCTTTGGTAGCTCATTAGACCAGATTGGACCTT
TTGCTCCTACTGTTAAGGAAAATGCCCTCTTGCTCAACGCTATTGCCAGCGAAGATGCTAAAGACTCTACTTCTGCTCCT
GTCCGCATCGCCGACTTTACTTCAAAAATCGGCCAAGACATCAAGGGTATGAAAATCGCTTTGCCTAAGGAATACCTCGG
TGAAGGAATTAACCCAGAGGTTAAGGAAACCATTCTAAATGCCGCTAAACACTTTGAAAAATTGGGTGCTATTGTCGAAG
AAGTCAGCCTTCCTCACTCTAAATACGGAGTTGCCGTATACTACATCATCGCTTCATCAGAAGCTTCATCAAACTTGCAA
CGCTTTGACGGTATCCGTTACGGCTATCGCGCAGAAGATGCAACCAACCTTGATGAAATCTATGTAAACAGCCGAAGCCA
AGGTTTTGGTGAAGAAGTGAAGCGCCGTATCATGCTGGGTACTTTCAGTCTTTCATCAGGTTACTACGATGCCTACTATA
AGAAGGCTGGACAGGTCCGTACACTTATCATTCAAGATTTCGAAAAAGTCTTCGCGGATTACGATTTGATTTTGGGTCCA
ACTGCTCCAAGTGTTGCCTATGACTTGGATTCTCTCAACCATGACCCAGTTGCCATGTACTTAGCCGACCTATTGACCAT
ACCTGTAAACTTGGCAGGACTGCCTGGAATTTCGATTCCTGCTGGATTCTCTCAAGGTCTACCTGTCGGTCTGCAATTGA
TTGGTCCCAAGCACTCTGAGGAAACCATTTACCAAGTTGCTGCTGCCTTTGAAGCAACAACAGGCTACCACAAACAACAA
CCCGTGATTTTTGGAGGTGACAACTAA

Upstream 100 bases:

>100_bases
AAGAAGGAATAGACCGTGATCGCTTGTTTAAAAACGTACCTGAAAAAGACAACTACTATATCAAGGTGCCAGCTATCCTA
GACAATGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
TGAACTTTGAAACAGTCATCGGACTTGAAGTCCACGTAGAGCTCAACACCAATTCAAAAATCTTCTCACCTACTTCTGCC
CACTTCGGAAATGATCAAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNI
STDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
VRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGP
TAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
PVIFGGDN

Sequences:

>Translated_488_residues
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNI
STDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
VRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGP
TAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
PVIFGGDN
>Mature_487_residues
TFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNIS
TDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVA
SGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAPV
RIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPT
APSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQP
VIFGGDN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=38.5375494071146, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.2545824847251, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=36.283185840708, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=24.8434237995825, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=182, Percent_Identity=32.4175824175824, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=321, Percent_Identity=24.9221183800623, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=188, Percent_Identity=31.9148936170213, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=266, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=266, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=458, Percent_Identity=32.9694323144105, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=30.5045871559633, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320448, Length=193, Percent_Identity=30.0518134715026, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=41.2244897959184, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24652985, Length=487, Percent_Identity=26.2833675564682, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI19922090, Length=487, Percent_Identity=26.2833675564682, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24652981, Length=487, Percent_Identity=26.2833675564682, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24652983, Length=487, Percent_Identity=26.2833675564682, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=24.7058823529412, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=23.8747553816047, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45550774, Length=522, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=522, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648437, Length=522, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=506, Percent_Identity=23.9130434782609, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648441, Length=454, Percent_Identity=24.2290748898678, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648439, Length=454, Percent_Identity=24.2290748898678, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52004; Mature: 51873

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKT
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECC
NMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
CCCCCCEECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEHHHCCCCCCCCCCC
VRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKETILNAAKHFEKLGAIVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHEEE
TFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPTAPSVAYDLDSLNHDPVAMY
EEECCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PVIFGGDN
CEEECCCC
>Mature Secondary Structure 
TFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKT
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECC
NMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
CCCCCCEECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEHHHCCCCCCCCCCC
VRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKETILNAAKHFEKLGAIVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHEEE
TFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPTAPSVAYDLDSLNHDPVAMY
EEECCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PVIFGGDN
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA