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The map label for this gene is hisS

Identifier: 225859879

GI number: 225859879

Start: 2037813

End: 2039102

Strand: Reverse

Name: hisS

Synonym: SP70585_2230

Alternate gene names: 225859879

Gene position: 2039102-2037813 (Counterclockwise)

Preceding gene: 225859880

Following gene: 225859878

Centisome position: 93.34

GC content: 46.43

Gene sequence:

>1290_bases
ATGAAATTACAAAAACCAAAAGGAACGCAGGATATTTTACCTGCTGAATCTGCCAAATGGCAGTACGTTGAGGGCTTTGC
CCGTGAAATTTTCAAGCGCTACAACTACGCAGAAGTGCGTACGCCTATTTTCGAGCATTACGAGGTTATCAGCCGCTCTG
TCGGAGATACAACAGATATCGTAACCAAGGAAATGTACGATTTTTATGACAAGGGTGACCGTCATATTACCCTCCGTCCA
GAAGGAACTGCGCCCGTTGTCCGTTCCTATGTGGAAAATAAACTCTTCGCCCCAGAAGTGCAAAAGCCAAGCAAGTTCTA
CTACATGGGACCTATGTTCCGTTATGAGCGTCCACAGGCAGGGCGCTTGCGCCAATTCCACCAGATTGGTGTTGAGTGTT
TTGGCTCTAGCAATCCAGCTACCGATGTGGAAACAATCGTTATGGCAGCCCATTTTTTGAAGGAAATCGGTATTCAAGGT
GTCAAATTGCACCTCAACACTCTTGGAAATCCTGAGAGCCGTGCAGCCTACCGCCAAGCCTTGATTGACTATTTGACACC
GCTCAAGGAGACCTTGTCTAAGGATAGCCAACGTCGCTTGGAGGAAAATCCTCTTCGTGTCTTGGACTCTAAGGAAAAAG
AAGACAAGGTGGCAGTAGAGAATGCGCCGTCTATCTTGGACTTTCTTGATGAAGAAAGCCAAACTCATTTTGATGCTGTG
CGTCAGATGTTGGAAAATCTTGGAGTAGATTACATCATCGATACCAATATGGTGCGTGGTCTGGACTACTACAACCACAC
CATTTTCGAGTTTATCACAGAGATTGAGGGCAATGACTTGACAGTCTGTGCGGGTGGTCGCTACGATGGTTTGGTTGCTT
ACTTTGGAGGCCCTGAAACTGCTGGATTTGGTTTTGGGCTTGGTGTAGAGCGCCTGCTTCTCATCCTTGAAAAACAAGGC
GTGGCCCTCCCTATCGAAAATGCCCTAGATGTCTATATCGCAGTCTTGGGTGATGGAGCAAATGTCAAAGCCCTAGAACT
AGTCCAAGCTCTTCGCCAACAAGGTTTCAAAGCAGAGCGTGATTACCTCAACCGTAAGCTCAAAGCTCAGTTCAAGTCAG
CCGATGTCTTTGCGGCTAAGACCCTCATCACCCTAGGAGAGAGCGAAGTCGAAAGCAGACAAGTGACAGTCAAGAACAAC
CAAACCCGAGAAGAAGTGCAAGTGTCACTTGAGACAATCAGCCAAAACTTCTCAGAAATCTTTGAAAAACTAGGATTTTA
TACTCAATAA

Upstream 100 bases:

>100_bases
GAGGTATGAATGATTTGGGTTAAATCCTAATTTTTTTCTCACTGTTTTAATCCCTTTATTTGTGGTAAAATAAGACTATT
AAGTTTAAAGAGGATTCCCT

Downstream 100 bases:

>100_bases
AAATCAAAGAGCAAACTAGGAAGCTAGCCGCAGGTTGCTCAAAACACTGTTTTGAGGTTGTGGATAGAACTGACGAAGTC
AGCTCAAAACATGGTTTTGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESRQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ

Sequences:

>Translated_429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESRQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ
>Mature_429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESRQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=478, Percent_Identity=25.5230125523013, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI6996014, Length=473, Percent_Identity=26.6384778012685, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=40.9952606635071, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=26.7391304347826, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=460, Percent_Identity=26.7391304347826, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=472, Percent_Identity=21.6101694915254, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24643061, Length=465, Percent_Identity=25.1612903225806, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643059, Length=484, Percent_Identity=24.3801652892562, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48754; Mature: 48754

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCH
GRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
LIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
HHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHH
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCC
AGFGFGLGVERLLLILEKQGVALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAER
CCCCCCHHHHHHHHHHHCCCCEECHHHHCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
DYLNRKLKAQFKSADVFAAKTLITLGESEVESRQVTVKNNQTREEVQVSLETISQNFSEI
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
FEKLGFYTQ
HHHHCCCCC
>Mature Secondary Structure
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCH
GRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
LIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
HHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHH
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCC
AGFGFGLGVERLLLILEKQGVALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAER
CCCCCCHHHHHHHHHHHCCCCEECHHHHCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
DYLNRKLKAQFKSADVFAAKTLITLGESEVESRQVTVKNNQTREEVQVSLETISQNFSEI
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
FEKLGFYTQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA